De novo transcriptome sequencing of Monodopsis subterranea CCALA 830 and identification of genes involved in the biosynthesis of eicosapentanoic acid and triacylglycerol
- 17 Downloads
Monodopsis subterranea, a unicellular yellow-green freshwater microalga, is widely known for its ability to produce high amount of therapeutically beneficial ω-3 polyunsaturated fatty acid eicosapentanoic acid (EPA; C20:5Δ5,8,11,14,17). Currently, no genomic information is available on M. subterranea despite its nutraceutical and commercial applications. Analysis on fatty acid methyl esters from M. subterranea strain CCALA 830 demonstrated accumulation of 28% EPA. In order to obtain better understanding of EPA biosynthesis and to identify genes involved in the process of lipid metabolism and accumulation in this alga, de novo transcriptome sequencing and assembly was performed using Illumina Hiseq 2000 sequencing. A total of 35,954 transcripts were obtained through final transcriptome assembly with an average transcript length of 769.36 bp. BLAST similarity searches for assembled transcripts were performed followed by annotation using Gene ontology and Kyoto Encyclopedia of Genes and Genomes orthology identifiers. Transcripts involved in lipid biosynthesis including various fatty acid desaturases and elongases involved in PUFAs biosynthesis were identified during the study. In addition, sequences for several transcription factors and a number of simple sequence repeats were also ascertained which can be used as powerful genetic markers for further genetic analysis. This study would provide a useful resource for future research on M. subterranea genome.
KeywordsMonodopsis subterranea Transcriptome Illumina HiSeq 2000 Fatty acid biosynthesis TAG EPA
Authors acknowledge financial support from the Department of Biotechnology, Ministry of Science and Technology, India (BT/PR6027/AGII/106/859/2012). SS is thankful for INSPIRE fellowship (IF140077) from the Department of Science and Technology, India.
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest.
- Fukuda S, Hirasawa E, Takemura T et al (2018) Accelerated triacylglycerol production without growth inhibition by overexpression of a glycerol-3-phosphate acyltransferase in the unicellular red alga Cyanidioschyzon merolae. Sci Rep. https://doi.org/10.1038/s41598-018-30809-8 CrossRefPubMedPubMedCentralGoogle Scholar
- Hibberd DJ (1981) Notes on the taxonomy and nomenclature of the algal classes Eustigmatophyceae and Tribophyceae (synonym Xanthophyceae). Bot J Linn Soc 82:93–119. https://doi.org/10.1111/j.1095-8339.1981.tb00954.x CrossRefGoogle Scholar
- Kaye Y, Grundman O, Leu S et al (2015) Metabolic engineering toward enhanced LC-PUFA biosynthesis in Nannochloropsis oceanica: overexpression of endogenous δ12 desaturase driven by stress-inducible promoter leads to enhanced deposition of polyunsaturated fatty acids in TAG. Algal Res 11:387–398. https://doi.org/10.1016/j.algal.2015.05.003 CrossRefGoogle Scholar
- Rismani-yazdi H, Haznedaroglu BZ, Bibby K, Peccia J (2011) Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: pathway description and gene discovery for production of next-generation biofuels. BMC Genomics 12:148. https://doi.org/10.1186/1471-2164-12-148 CrossRefPubMedPubMedCentralGoogle Scholar
- Rismani-Yazdi H, Haznedaroglu BZ, Hsin C, Peccia J (2012) Transcriptomic analysis of the oleaginous microalga Neochloris oleoabundans reveals metabolic insights into triacylglyceride accumulation. Biotechnol Biofuels 5:74. https://doi.org/10.1186/1754-6834-5-74 CrossRefPubMedPubMedCentralGoogle Scholar
- Santos LM, Leedale GF (1995) Some notes on the ultrastructure of small azoosporic members of the algal class Eustigmatophyceae. Nov Hedwigia 60:219–225Google Scholar
- Sato A, Matsumura R, Hoshino N et al (2014) Responsibility of regulatory gene expression and repressed protein synthesis for triacylglycerol accumulation on sulfur-starvation in Chlamydomonas reinhardtii. Front Plant Sci. https://doi.org/10.3389/fpls.2014.00444 CrossRefPubMedPubMedCentralGoogle Scholar
- Yu M, Yang S, Lin X (2016) De-novo assembly and characterization of Chlorella minutissima UTEX2341 transcriptome by paired-end sequencing and the identification of genes related to the biosynthesis of lipids for biodiesel. Mar Genomics 25:69–74. https://doi.org/10.1016/j.margen.2015.11.005 CrossRefPubMedGoogle Scholar