, Volume 32, Issue 4, pp 635–642 | Cite as

Phyto-chemical composition of the ethno-medicinal plant Priva cordifolia (L.f) Druce

  • A. P. AnandaEmail author
  • N. B. KrishnamurthyEmail author
  • B. S. Nagendra
  • K. R. Savitha
Research Articles


The aim of the study was to determine the phytochemical composition of the Indian ethno-medicinal plant Priva cordifolia (L.f) Druce. Standard methods were adopted to perform a proximate analysis of P. cardifolia to determine its phytochemical and mineral contents. Fatty acids and vitamin-C were analyzed by GC-FID and HPLC respectively. β-carotene was characterized by FTIR spectroscopy. A total phenolic content of 102 ± 1.14 mg/g and flavonoids, 350.48 ± 12.80 mg/g were found in the plant’s leaf extract. The concentration of crude fat, ash, crude protein, crude fiber, and carbohydrate were respectively 2.08 ± 0.10, 16.32 ± 0.52, 19.04 ± 0.62, and 9.1 ± 0.14 and 41.64 ± 1.20 g/100 g of dried leaf powder. The content of Ca, K and Mg were 2249.38 ± 66.00, 2151.51 ± 60.25 and 356.15 ± 2.95 mg/100 g respectively, and that of the trace elements Fe, Zn, Cu, and Mn 94.01 ± 2.10, 9.40 ± 0.12, 1.66 ± 0.06 and 6.23 ± 0.09 mg/100 g of dried leaf powder respectively. The polyunsaturated fatty acid- linoleic, linolenic, and arachidonic acids content found in extracted fat was 16.42, 46.62 and 0.72 g/100 g respectively. β-Carotene 894.00 ± 12.00 µg/g and vitamin-C 53.34 ± 1.60 µg/g of fresh leaves were found. The data seem to suggest the possibility of a correlation of the phyto-chemical composition of P. cordifolia with some of its already known therapeutic properties. The total phenolics and antioxidants of this plant, in particular, may play the key role in relief from oxidation stress and in wound healing.


Proximate analysis Minerals Total phenolics β-carotene Fatty acid profile 



The authors wish to acknowledge the financial support and facilities kindly extended by the CEO of Ganesh Consultancy and Analytical Services, Laboratory, Mysuru.

Compliance with ethical standards

Conflict of interest

We declare that we have no conflict of interest.

Human and animal statements

This article does not contain any studies with human or animal subjects.


  1. Ananda AP, Sampath Kumara KK, Nagendra BS, Savitha KR, Krishnamurthy NB (2016) A taxonomical note on Priva cordifolia (L.f) Druce an Indian ethno-medicinal plant. World J Pharm Pharm Sci. 5(8):356–363Google Scholar
  2. Ananda AP, Manukumar HM, Krishnamurthy NB, Nagendra BS, Savitha KR (2019a) Assessment of antibacterial efficacy of a biocompatible nanoparticle PC@ AgNPs against Staphylococcus aureus. Microb Pathog 126:27–39CrossRefGoogle Scholar
  3. Ananda AP, Krishnamurthy NB, Nagendra BS, Savitha KR (2019b) Biogenic synthesis of silver nanoparticles using Priva cordifolia leaf extract (PC@AgNPs) a potent antioxidant, antibacterial and catalytic activity. SN Appl Sci. CrossRefGoogle Scholar
  4. Anyasor GN, Ogunwenmo O, Oyelana OA, Akpofunure BE (2010) Phytochemical constituents and antioxidant activities of aqueous and methanol stem extracts of Costusafer Ker Gawl(Costaceae). Afr J Biotechnol 9(31):4880–4884Google Scholar
  5. Atanasov AG, Waltenberger B, Pferschy-Wenzig EM et al (2015) Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnol Adv 33(8):1582–1614. CrossRefPubMedPubMedCentralGoogle Scholar
  6. Awad AB, Roy R, Fink CS (2003) β-sitosterol, a plant sterol, induces apoptosis and activates key caspases in MDA-MB-231 human breast cancer cells. Oncol Rep 10(2):497–500PubMedGoogle Scholar
  7. Ayyanar M, Ignacimuthu S (2009) Herbal medicines for wound healing among tribal people in Southern India: ethnobotanical and Scientific evidences. Int J Appl Res Nat Prod 2(3):29–42Google Scholar
  8. Bouic PJ (2001) The role of phytosterols and phytosterolins in immune modulation: a review of the past 10 years. Curr Opin Clin Nutr Metabol Care 4(6):471–475CrossRefGoogle Scholar
  9. Chang CC, Yang MH, Wen HM, Chern JC (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10(3):178–182Google Scholar
  10. Harborne JB (1998) Photochemical methods: a guide to modern techniques of plant analysis, 2nd edn. London, UK, Chapman A. & Hall, pp 4–84Google Scholar
  11. Hong L, Guo Z, Huang K et al (2015) Ethnobotanical study on medicinal plants used by Maonan people in China. J Ethnobiol Ethnomed 11:32. CrossRefPubMedPubMedCentralGoogle Scholar
  12. Houston MC, Harper KJ (2008) Potassium, magnesium, and calcium: their role in both the cause and treatment of hypertension. J Clin Hypertens 10(7):3–11CrossRefGoogle Scholar
  13. HMSO (1994) U.K. Nutritional Aspect of Cardiovascular Disease, vol 46. Department of Health, Report on health and Social Subjects, London, pp 37–46Google Scholar
  14. Kaur C, Kapoor HC (2001) Antioxidants in fruits and vegetables—the millennium’s health. Int J Food Sci Technol 36(7):703–725CrossRefGoogle Scholar
  15. Koca N, Burdurlu HS, Karadeniz F (2007) Kinetics of colour changes in dehydrated carrots. J Food Eng 78(2):449–455CrossRefGoogle Scholar
  16. Kokate KC (1997) Practical pharmacognacy, 4th edn. Vallabh Prakashan, Delhi, p 218Google Scholar
  17. Manukumar HM, Ananda AP, Vishwanathan D (2013) Study of physicochemical parameters and antioxidant in honey collected from different locations of India. Int J Pharm Life Sci 4(12):3159–3165Google Scholar
  18. Mishra KP, Ganju L, Sairam M, Banerjee PK, Sawhney RC (2008) A review of high throughput technology for the screening of natural products. Biomed Pharmacother 62(2):94–98CrossRefGoogle Scholar
  19. Moghadasian MH, McManus BM, Pritchard PH, Frohlich JJ (1997) “Tall oil”–derived phytosterols reduce atherosclerosis in ApoE-deficient mice. Arterioscler Thromb Vasc Biol 17(1):119–126CrossRefGoogle Scholar
  20. Moghadasian MH, McManus BM, Godin DV, Rodrigues B, Frohlich JJ (1999) Proatherogenic and antiatherogenic effects of probucol and phytosterols in apolipoprotein E–deficient mice: possible mechanisms of action. Circulation 99(13):1733–1739CrossRefGoogle Scholar
  21. Mohan RK, Bhirava Murthy PV (1992) Plants used in traditional medicine by tribals of prakasam district. Andhra Pradesh. Ancient Sci Life 11(3–4):176Google Scholar
  22. Panday SB, Tiwari MR (2002) Ruminant livestock production and their role in sustainable development in Mountain Regions of Nepal. In: Wangdi K, Roder W, Gyaltsen T (eds) Sustainable mountain development-agro-pastoral systems and fodder crops in the Himalayan region. Proceedings of the fifth Meetings of the Temperate Asia Pasture and Fodder network (TAPAFON), held at Renewable Natural Resources Research Centre, Bajo, Wangdue, Bhutan, 29 Apr to 4 May 2002Google Scholar
  23. Ranganna S (2009) Handbook of analysis and quality control for fruit and vegetable products, 2nd edn. Tata McGraw-Hill publishing company, New DelhiGoogle Scholar
  24. Revathy SS, Rathinamala R, Murugesan M (2012) Authentication methods for drugs used in ayurveda, siddha and unani systems of medicine: an overview. Int J Pharm Sci Res 3(8):2352–2361Google Scholar
  25. Samuelsson B, Granstrom E, Green K, Hamberg M (1971) Metabolism of prostaglandins. Ann NY Acad Sci 180(13):8–163Google Scholar
  26. Singh M (2005) Essential fatty acids, DHA and human brain. Indian J Pediatr 72(3):239–242CrossRefGoogle Scholar
  27. Soetan KO, Olaiya CO, Oyewole OE (2010) The importance of mineral elements for humans, domestic animals and plants—a review. Afr J Food Sci 4(5):200–222Google Scholar
  28. Spanos GA, Wrolstad RE (1990) Influence of processing and storage on the phenolic composition of Thompson seedless grape juice. J Agric Food Chem 38(7):1565–1571CrossRefGoogle Scholar
  29. Swanson Danielle, Block Robert, Mousa Shaker A (2012) Omega-3 fatty acids EPA and DHA: health benefits throughout life. Adv Nutr Int Rev J 3(1):1–7CrossRefGoogle Scholar
  30. Van Rensburg SJ, Daniels WMU, Van Zyl JM, Taljaard JJF (2000) A Comparative study of the effects of cholesterol, beta-sitosterol, beta-sitosterol glucoside, dehydro-epiandrosterone sulphate and melatonin on in vitro lipid peroxidation. Metab Brain Dis 15(4):257–265CrossRefGoogle Scholar
  31. Witham FH, Blaydes DF, Devlin RM (1971) Experiments in plant physiology. Van Nostrand Reinhold, New York, pp 241–242Google Scholar
  32. Zahin M, Aqil F, Ahmad I (2009) The in vitro antioxidant activity and total phenolic content of four Indian medicinal plants. Int J Pharm Pharm Sci 1(1):88–95Google Scholar

Copyright information

© Society for Plant Research 2019

Authors and Affiliations

  1. 1.Research and Development Centre, Bharathiar UniversityCoimbatoreIndia
  2. 2.Ganesh Consultancy and Analytical ServicesHebbal Industrial AreaMysuruIndia
  3. 3.Department of BiotechnologyShridevi Institute of Engineering and TechnologyTumkurIndia
  4. 4.Department of Chemistry, University College of ScienceTumkur UniversityTumkurIndia

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