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Identification of drought stress-responsive proteins in common bean

  • Nancy Gupta
  • Sajad Majeed ZargarEmail author
  • R. K. Salgotra
  • Tanveer A. Dar
Original Article
  • 4 Downloads

Abstract

Proteomics has emerged as a vital tool for high throughput systematic analysis of protein expression which helps to unlock various biochemical pathways. Keeping in view the relevance of differential expression studies at the post transcriptional level, we attempted to explore the candidate proteins that are triggered under low water stress conditions at vegetative and reproductive stages in common bean. The evaluation of drought-responsive proteins was performed by resolving the quantified protein extracted from leaf samples using SDS PAGE for detection of differential bands followed by trypsin digestion and MALDI-MS analysis. We report the presence of seven significant proteins where five proteins (Glucan endo-1.3 beta-glucosidase, Auxin-responsive protein, Endochitinase, Endochitinase CH5B and L-type lectin domain containing receptor kinase IV.3) were upregulated and two proteins (Ribulose bisphosphate carboxylase/oxygenase activase, ADP-ribosylation factor-like protein 8a) were down regulated. The study conducted herein indicates the strong interaction and varied activation of proteins at both the developmental stages. The identified proteins are primarily localized in nucleus, cell membrane, cytoskeleton, vacuole and chloroplast and play significant roles in plant defense, photosynthesis, and various molecular regulatory processes.

Keywords

Common bean Differential expression Drought MALDI-MS 

Notes

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Nancy Gupta
    • 1
  • Sajad Majeed Zargar
    • 2
    Email author
  • R. K. Salgotra
    • 1
  • Tanveer A. Dar
    • 3
  1. 1.School of BiotechnologyS K University of Agricultural Sciences and Technology of JammuJammuIndia
  2. 2.Division of Plant BiotechnologyS K University of Agricultural Sciences and Technology of SrinagarSrinagarIndia
  3. 3.Department of Clinical BiochemistryUniversity of KashmirSrinagarIndia

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