Occurrence of Colletotrichum fruticola along with C. gloeosporioides in causing anthracnose disease on Citrus sinensis in Tunisia
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Information on the Colletotrichum species associated with Citrus anthracnose was lacking. Surveys were conducted from January 2012 to February 2013 on different citrus species and varieties orchards in the Cap-Bon region (Tunisia) to investigate the prevalence of Colletotrichum species. Typical anthracnose symptoms such as dieback and wither-tip of twigs were noticed. The fungi associated with anthracnose were isolated and studied. Morphological features (mycelial and sporulation characterization), molecular identification by using ITS1 and ITS2 primers were done along with pathogenicity test monosporic cultures. Eight Colletotrichum isolates were collected from infected fruits, leaves and twigs and were identified as Colletotrichum gloeosporioides (seven isolates) and Colletotrichum fructicola (one isolate). All eight Colletotrichum isolates were pathogenic on Thomson, Malti and Meski orange varieties, Lunari lemon variety and sweet lime Bergamot detached fruit and leaves. This investigation revealed the prevalence, for the first time of C. fructicola, on Citrus in Tunisia.
KeywordsCap-Bon region Morphological features ITS Colletotrichum gloeosporioides C. fructicola Pathogenicity test
This study was financed by UR13AGR03, University of Sousse, Tunisia. We are grateful to José Sánchez and Eduardo Gallego, for their help.
It was a team work and all authors shared their respective expertise in drafting and approved the final draft of the manuscript for submission.
This research was supported by UR13AGR03, University of Sousse, Tunisia. The experiments comply with the current laws of the country in which they were performed.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Informed consent: Informed consent was obtained from all individual participants included in the study.
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