Morphology and phylogeny of a new species of Trichomerium from North-Western Himalayas, India

  • Shiwali Rana
  • Sanjay K. SinghEmail author
  • Paras N. Singh
Research Article


The present paper describes Trichomerium bhatii, a new species in the family Trichomeriaceae (Chaetothyriales, Ascomycota). This taxon was isolated as epiphyte from dead bark of Prunus cerasoides collected from North-Western Himalayan region of India. The identity of isolate was based on asexual-morphs, cultural characteristics and phylogenetic analyses of partial nuclear ribosomal 28S large subunit and complete internal transcribed spacer rDNA sequence data with high statistical support value. The new taxon has turned out to be distinct from other known species in the genus showing 95.26% (624/655 bp) identity with closely related T. gloeosporum MFLUCC10-0087 on the basis of rDNA internal transcribed spacer region. A brief description of asexual-morph of T. bhatii with morphological and molecular data is provided. To the best of our knowledge this is the first report on this genus from India. The type specimen is deposited in the Ajrekar Mycological Herbarium (as voucher culture) and ex type cultures are deposited in Indian Type Culture Collection (ITCC-WDCM 430), New Delhi, and National Fungal Culture Collection of India (NFCCI-WDCM 932).


Asexual morph ITS LSU Trichomerium Sooty molds Trichomeriaceae 



Authors thank the Director MACS’ Agharkar Research Institute, Pune for providing research facilities. Ms Shiwali Rana acknowledges University Grant Commission (UGC), New Delhi for granting Junior Research Fellowship (JRF).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.


  1. Aamir S, Sutar S, Singh SK, Baghela A (2015) A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR based molecular methods. Plant Pathol Q J 5(2):74–81Google Scholar
  2. Batista AC (1951) Alguns fungos de fumagina de Pernambuco. Mycopathol Mycol Appl 5(2–3):147–172CrossRefGoogle Scholar
  3. Batista AC, Ciferri R (1955) Novos elementos para a taxonomia dos fungos de fumagina. Anais da Sociedade de Biologia de Pernampuco 13(2):108–124Google Scholar
  4. Batista AC, Ciferri R (1963) Capnodiales. Saccardoa 2:1–296Google Scholar
  5. Chomnunti P, Schoch CL, Aguirre-Hudson B, Ko Ko TW, Hongsanan S, Jones EBG, Kodsueb R, Phookamsak R, Chukeatirote E, Bahkali AH, Hyde KD (2011) Capnodiaceae. Fungal Divers 51:103–134. CrossRefGoogle Scholar
  6. Chomnunti P, Bhat DJ, Gareth Jones EB, Chukeatirote E, Bahkali AH, Hyde KD (2012) Trichomeriaceae, a new sooty mold family of Chaetothyriales. Fungal Divers 56(1):63–76. CrossRefGoogle Scholar
  7. Chomnunti P, Hongsanan S, Hudson BA et al (2014) The sooty moulds. Fungal Divers 66:1–36CrossRefGoogle Scholar
  8. Crous PW, Wingfield MJ, Schumacher RK et al (2014) Fungal planet description sheets: 281–319. Persoonia 33:212–289. CrossRefGoogle Scholar
  9. Crous PW, Wingfield MJ, Burgess TI et al (2017) Fungal Planet description sheets: 625–715. Persoonia. 39:270–467. Google Scholar
  10. Hongsanan S, Tian Q, Hyde KD, Hu DM (2016) The asexual morph of Trichomerium gloeosporum. Mycosphere 7(9):1473–1479. CrossRefGoogle Scholar
  11. Hughes SJ (1976) Sooty molds. Mycologia 68:693–820. CrossRefGoogle Scholar
  12. Hyde KD, Hongsanan S, Jeewon R et al (2016) Fungal diversity notes 367–490: taxonomic and phylogenetic contributions to fungal taxa. Fungal Divers 80:1–270. CrossRefGoogle Scholar
  13. Isola D, Zucconi L, Onofri S, Santamaria U et al (2016) Extremotolerant rock-inhabiting black fungi from Italian monumental sites. Fungal Divers 76:75–96. CrossRefGoogle Scholar
  14. Katoh K, Asimenos G, Toh H (2009) Multiple alignment of DNA sequences with MAFFT. Methods Mol Biol 537:39–64CrossRefGoogle Scholar
  15. Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120CrossRefGoogle Scholar
  16. Kornerup A, Wanscher JH (1978) Metheun’s Handbook of colours, 3rd edn. Metheun and Co Ltd., London, p 252Google Scholar
  17. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  18. Liu JK, Hyde KD, Jones EBG et al (2015) Fungal diversity notes 1–100: taxonomic and phylogenitic contribution to fungal species. Fungal Divers 72:1–197. CrossRefGoogle Scholar
  19. Nascimento MMF, Selbmannc L, Sharifyniab S, Al-Hatmib AMS et al (2016) Arthrocladium, an unexpected human opportunist in Trichomeriaceae (Chaetothyriales). Fungal Biol 120:207–218. CrossRefGoogle Scholar
  20. Spegazzini C (1918) Notas micológicas. Phys Rev de la Soc Argent de Ciencias Nat 4(17):281–295Google Scholar
  21. Spegazzini C (1924) Algunos honguitos portoriqueños. Boletín de la Acad Nacional de Ciencias en Córdoba 26(2–4):335–368Google Scholar
  22. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246CrossRefGoogle Scholar
  23. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and application. Academic Press, San Diego, pp 315–322Google Scholar

Copyright information

© Indian Phytopathological Society 2019

Authors and Affiliations

  1. 1.National Fungal Culture Collection of India, Biodiversity and Palaeobiology GroupMACS’ Agharkar Research InstitutePuneIndia

Personalised recommendations