Antioxidants elevates the resistance to Cercospora canescens in interspecific cross of Vigna radiata (Kopergaon) × Vigna mungo (Pant Urd 31)
Cercospora leaf spot (CLS) caused by Cercospora canescens is one of the most important foliar diseases responsible for 30%–75% yield losses in mungbean. CLS is induced by the photo-activated toxin cercosporin. The most operative measure of managing the disease is the use of resistant varieties. The objective of present study was to determine the changes in the antioxidants status of mung-bean plant to differential responses of Cercospora leaf spot under field conditions. The present study attempted to quantify the antioxidant activities against C. canescens infection in resistant RILs of inter specific cross of Vigna radiata (Kopergaon) × Vigna mungo (PU31). It was found that antioxidants such as superoxide dismutase (SOD) and catalase (CAT) enzymes were actively involved in scavenging ROS in both resistant and susceptible recombinant inbred lines (RILs) after CLS infection. Throughout the infection, the activity of both the enzymes were higher in resistant than in susceptible RILs. The malondialdehyde (MDA) accumulated at a significantly low concentration in resistant RILs and higher in susceptible ones. Statistically, area under disease progress curve (AUDPC) and MDA showed a significant positive correlation whereas, there was a negative correlation between AUDPC and SOD and CAT activity. The disease components such as lesion number and total lesion size were variable in RILs with different disease reactions. The study identified few RILs resistant to Cercospora leaf spot infection in response to utilization of the antioxidant system to confer Cercospora leaf spot resistance in mungbean by detoxifying cercosporin induced reactive oxygen species.
KeywordsCercospora leaf spot Cercosporin Reactive oxygen species Host plant resistance Mungbean
Sudhir Navathe is thankful to Department of Science and Technology (DST), Government of India for financial support in form of INSPIRE fellowship (IF 150037).
Ramesh Chand and Sudhir Navathe contributed to experimental design, compilation and data analysis. Anuj Kumar Maurya and Sudhir Navathe performed experiment. Chinmayee Mohapatra helped in data analysis, interpretation and manuscript writing.
Compliance with ethical standards
Conflict of interest
All authors have read the manuscript and they declare no conflict of interest.
- Aebi HE (1983) Catalase. In: Bergmeyer HU (ed) Methods in enzymatic analysis. Academic Press, New York, pp 276–286Google Scholar
- Bhat NA, Maheshwari SK, Ahmad S, Beig MA, Masoodi SD (2008) Field evaluation of mungbean accessions against Cercospora leaf spot. Ann Bot 24:1–9Google Scholar
- Chand R, Kumar P, Singh V, Pal C (2013) Technique for spore production in Cercospora canescens. Indian Phytopathol 66:159–163Google Scholar
- Gorinova N, Batchvarova R, Atanassov A (1998) Effect of cercosporin on antioxidant enzymes and ion leakage in toxin-sensitive and resistant cultivars of rice (Oryza sativa). Biol Br 53:727Google Scholar
- Grewal JS (1978) Diseases of Mungbean in India. In: Proceedings of the first international Mungbean symposium, Los Baños, pp 65–168Google Scholar
- Iqbal U, Iqbal SM, Zahid MA, Khan SH (2009) Screening of local Mungbean germplasm against Cercospora leaf spot disease. Pak J Phytopathol 21:123–125Google Scholar
- Kappus H (1985) Lipid peroxidation, mechanisms, analysis, enzymology and biological relevance. Oxidative Stress, Academic Press, London, pp 273–310Google Scholar
- Kumar RR, Goswami S, Singh K, Rai GK, Rai RD (2013) Modulation of redox signal transduction in plant system through induction of free radical/ROS scavenging redox-sensitive enzymes and metabolites. Aust J Crop Sci 7:1744–1751Google Scholar
- Levene H (1960) Robust tests for equality of variances. Contrib Probab Stat Essay Honor Harold Hotel 2:278–292Google Scholar
- Misra DK, Bhattacharyya P (2002) Studies on growth and sporulation of Cercospora canescens. J Mycopathol Res 40:197–199Google Scholar
- Nair A, Abraham TK, Jaya DS (2008) Studies on the changes in lipid peroxidation and antioxidants in drought stress induced Cowpea (Vigia unguiculata L.) varieties. J Environ Biol 29:689–691Google Scholar
- Poehlman JM (1978) What we have learned from the International Mungbean Nurseries. In: First international Mungbean symposium, Los Banos (Philippines), 16 Aug 1977. Office of Information Services, AVRDCGoogle Scholar
- Poehlman JM (1991) The Mungbean. Oxford and IBH Publ, New Delhi, p 19Google Scholar
- Quebral FC, Cagampang IC (1970) Influence of Cercospora leafspot control on yield of mungbean. Agr Los Banos. 9:13–14Google Scholar
- Rachie KO, Roberts LM (1974) Grain legumes of the lowland tropics. Adv Agron 26:62–77Google Scholar
- Sahoo BK, Hota AK (1991) Field screening of Greengram germplasm against insect pest and disease complex. Mad Agric J 78:84–86Google Scholar
- Sheng XC, Qin XS, Qiang HH, Chen GYJ (2008) Influence of Cercospora zeae-maydis toxin on defense enzyme activities and its function in the induced resistance of corn. J N W A F Univ Nat Sci Ed 36:175–180Google Scholar
- Silva JP, Coutinho OP (2010) Free radicals in the regulation of damage and cell death—basic mechanisms and prevention. Drug Discov Ther 4:144–167Google Scholar
- Steinkamp MP, Martin SS, Hoefert LL, Ruppel EG (1981) Ultrastructure of lesions produced in leaves of Beta vulgaris by cercosporin, a toxin from Cercospora beticola. Phytopathology 71:1272–1281Google Scholar
- Thirumaran AS, Seralathan MA (1988) Utilization of Mungbean. In: Hanmugasundram S, McLean BT (eds), Mungbean, Proceedings of the second international symposium Shanhua, Taiwan, Asian Vegetable Research and Development Center, AVRDC Publication No 88-304:470–485Google Scholar
- Upchurch RG, Walker DC, Rollins JA, Ehrenshaft M, Daub ME (1991) Mutants of Cercospora kikuchii altered in cercosporin synthesis and pathogenicity. Appl Environ Microbiol 57:2940–2945Google Scholar
- Williamson JD, Scandalios JG (1992) Differential response of Maize catalases and superoxide dismutases to the photo-activated fungal toxin cercosporin. Plant J 2:351–358Google Scholar