Influence of different SAR elicitors on induction and expression of PR-proteins in Potato and Muskmelon against Oomycete pathogens

  • AsthaEmail author
  • P. S. Sekhon
  • M. K. Sangha
Research Article


Two cultivars each of potato namely; Kufri Badshah, Kufri Jyoti and muskmelon namely; Kajri and Punjab Hybrid were treated with varying concentrations of four different elicitors for induction of systemic acquired resistance (SAR) against Phytophthora infestans causing late blight in potato and Pseudoperonospora cubensis causing downy mildew in muskmelon. Attempts were made to induce SAR with application of various SAR elicitors as spray i.e. Jasmonic acid (JA), Salicylic acid (SA), Benzothiadiazole (BTH) and Beta Amino butyric acid (BABA). These elicitors were sprayed on 3-week-old sprouts and effect on defence related proteins along with late blight and downy mildew severity was studied. Leaf samples were collected up to 7 days post treatment and used for estimation of total proteins. Sporangial solution (4.0 × 104 sporangia per ml) was sprayed after 1 week of elicitors spray. Per cent disease severity was recorded at 14 days post inoculation. SA, JA, BTH and BABA caused statistically significant (at 5% level) increase in total soluble protein in leaf samples. The best treatments for maximum induction of proteins were 500 μM each of SA, JA, and BTH and 50 mM for BABA. SDS–PAGE electrophoresis confirmed that exogenous application of JA, SA, BABA and BTH resulted in the induction of PR proteins of low molecular size along with some other proteins in both potato and muskmelon, which helped in induction of SAR against oomycete pathogens.


SAR Phytophthora infestans Pseudoperonospora cubensis Salicylic acid Jasmonic acid Benzothiadiazole and Beta amino butyric acid PR-proteins Lesion length 


  1. Agamy R, Alamri S, Moustafa MFM, Hashem M (2013) Management of tomato leaf spot Caused by Alternaria tenuissima using salicylic acid and Agrileen. Int J Agric Biol 15:266–272Google Scholar
  2. Aldesuquy HS (2015) Shikimic acid and salicylic acid induced protection on growth vigor, seed yield and biochemical aspects of yielded seeds of Vicia faba plants infected by Botrytis fabae. J Pl Pathol Microb 6(9):65–78Google Scholar
  3. Andreu AB, Guevara MG, Wolski EA, Daleo GR, Caldiz AD (2006) Enhancement of natural disease resistance in potatoes by chemicals. Pest Manag Sci 62:162–170CrossRefGoogle Scholar
  4. Anonymous (1987) CPRI: Annual scientific report, pp 91–97Google Scholar
  5. Bains SS, Jhooty JS (1976) Over wintering of Pseudoperonospora cubensis causing downy mildew of muskmelon. Indian Phytopathol 29:213–214Google Scholar
  6. El-Khallal SM (2007) Induction and modulation of resistance in tomato plants against Fusarium wilt disease by bioagent fungi (arbuscular mycorrhiza) and/or hormonal elicitors (jasmonic acid and salicylic acid): changes in growth, some metabolic activities and endogenous hormones related to defense mechanism. Aust J Basic Appl Sci 1:691–705Google Scholar
  7. Enkerli J, Gisi U, Mosinges E (1993) Systemic acquired resistance to Phytophora infestans in tomato and the role of pathogenesis-related proteins. Physiol Mol Plant Pathol 43:161–171CrossRefGoogle Scholar
  8. Esmailzadeh M, Soleimani MJ, Rouhani H (2008) Exogenous application of salicylic acid for inducing systemic acquired resistance against tomato stem canker disease. J Biol Sci 8:1039–1044CrossRefGoogle Scholar
  9. Gorlach J, Volrath S, Knauf-Beiter G, Henry G, Beckhove U (1996) Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease Resistance in wheat. Plant Cell 8:629–643CrossRefGoogle Scholar
  10. Haggag WM, Abd-El-Kareem F (2010) Methyl jasmonate stimulates polyamines biosynthesis and resistance against leaf rust in wheat plants. Arch Phytopathol Pl Prot 42:16–31CrossRefGoogle Scholar
  11. Jishan N, Jing L, Wenben M, Qiao YL, Zhengyang W, Dexian H (2011) The relationship of methyl jasmonates enhanced powdery mildew resistance in wheat and expression of 9 disease resistance related genes. Agric Sci Technol 12:504–508Google Scholar
  12. Kone AS, Csinos KL, Jackson PJ (2009) Evaluation of systemic acquired resistnce in plants and its inducers for control of Phytophthora capsici on squash. Crop Protec 28:6533–6538CrossRefGoogle Scholar
  13. Kumar S, Thind TS, Bala A, Gupta AK (2010) Induced resistance in potato against P infestans using chemicals and bio-agents. Pl Des Res 25(1):12–18Google Scholar
  14. Lebeda A, Cohen Y (2011) Cucurbit downy mildew (Pseudoperonospora cubensis)—biology, ecology, epidemiology, host-pathogen interaction and control. Eur J Plant Pathol 129:157–192CrossRefGoogle Scholar
  15. Lowry OH, Rosebrough NJ, Furr AL, Randal RJ (1951) Protein measurement with folin-phenol reagent. J Boil Chem 193:265–275Google Scholar
  16. McDowell JM, Dangl JL (2000) Signal transduction in the plant immune response. Trends Biochem Sci 25:79–82CrossRefGoogle Scholar
  17. Palti J, Cohen Y (1980) Downy mildew of cucurbits (Pseudoperonospora cubensis): the fungus and its hosts, distribution, epidemiology, and control. Phytoparasitica 8:109–147CrossRefGoogle Scholar
  18. Ryals JA, Neuenschwander UH, Willits MG, Molina A, Steiner HY (1996) Systemic acquired resistance. Plant cell 8:439–450CrossRefGoogle Scholar
  19. Thind TS, Mohan C (1998) Severity of late blight and assessment of yield losses in potato during 1997–98 epiphytotic in Punjab. Plant Dis Res 13:204–205Google Scholar
  20. Thomas CE (1996) Downy mildew: compendium of cucurbit diseases. American Phytopathol Soc Press, St. Paul, pp 25–27Google Scholar
  21. Van Loon LC (1983) The induction of pathogenesis-related-proteins by pathogens and specific chemicals. Neth J Plant Pathol 89:265–273CrossRefGoogle Scholar
  22. Walker JM (1996) SDS polyacrylamide gel electrophoresis of proteins. In: Walker JM (ed) The protein protocols handbook. Humana, Totowa, pp 55–61CrossRefGoogle Scholar
  23. Wang B, Liu J, Tian Z, Song B, Xie C (2005) Monitoring the expression patterns of potato genes associated with quantitative resistance to late blight during P. infestans infection using cDNA microarrays. Pl Sci 169:1155–1167CrossRefGoogle Scholar

Copyright information

© Indian Phytopathological Society 2019

Authors and Affiliations

  1. 1.Department of Plant PathologyPunjab Agricultural UniversityLudhianaIndia
  2. 2.Department of BiochemistryPunjab Agricultural UniversityLudhianaIndia

Personalised recommendations