Effect of physiological factors on production of cellulolytic enzymes by Rhizoctonia bataticola
Cellulose, an abundant carbohydrate, forms an integral part of the plant cell walls and provides structural integrity. Ability of any pathogenic fungus to degrade it depends largely on the extent of cellulolytic enzymes produced by a fungus. Therefore, present in vitro study was conducted to assess the effect of five each carbon and nitrogen sources on production of cellulolytic enzymes and growth of Rhizoctonia bataticola isolates the dry root rot pathogen of soybean. The results revealed that cellulolytic enzyme activity (µg of d-glucose/ml) of the 20 isolates of R. bataticola was strongly influenced and varied with the sources of carbon and nitrogen tested. Among five carbon sources tested, the cellulolytic enzyme activity was highest with Carboxy methyl cellulose (CMC) in the range of 0.629–1.286 µg, followed by Glucose (0.589–0.996 µg), Sucrose (0.549–0.961 µg), Starch (0.199–0.797 µg) and Pectin (0.152–0.293 µg) compared to control (0.107–0.187 µg). Similarly, all five nitrogen sources tested exhibited a wide range of cellulolytic enzyme activity among the test isolates. However, it was highest with Ammonium chloride (0.196–0.420 µg), followed by Potassium nitrate (0.170–0.425 µg), Glutamic acid (0.118–0.174 µg), Glutamine (0.109–0.171 µg) and Urea (0.100–0.167 µg), compared to control (0.080–0.107 µg).Various temperature regimes and pH of CMC broth medium also influenced the growth (mycelium dry weight) of the test isolates of R. bataticola and their cellulolytic enzyme activity. However, the temperature of 30 °C and 6.0 pH where found to be optimum.
KeywordsRhizoctonia bataticola Cellulolytic enzyme Carbon Nitrogen Temperature pH
The authors are thankful to the Dean, Post Graduate Institute and Head, Department of Plant Pathology and Agriculture Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar, Maharashtra, India, for the facilities provided to this works.
- Adeleke AJ, Odunfa SA, Olanbiwonninu A, Owoseni MC (2012) Production of cellulase and pectinase from orange Peels by Fungi. Nat Sci 10(5):107–112Google Scholar
- Charul Kanchan, Biswas SK (2009) Morphological and pathogenic variability of Rhizoctonia bataticola (Taub) Butler, causal agent of leaf spot and blight disease of Pigeonpea. Ann Pl Prot Sci 17(1):124–126Google Scholar
- Chaurasia AK, Chaurasia S, Chaurasia S, Chaurasia S (2015) Influence of culture conditions on cellulase production by Sclerotium rolfsii Sacc. Internat J Multidisc Res Dev 2(5):41–49Google Scholar
- El-Said AHM, Saleem A, Maghraby TA, Hussein MA (2014) Cellulase activity of some phytopathogenic fungi isolated from diseased leaves of broad bean. Int J Curr Microbiol App Sci 3(2):883–900Google Scholar
- Gautam SP, Bundela PS, Pandey AK, Khan Jamaluddin, Awasthi MK, Sarsaiya S (2011) Optimization for the production of cellulase enzyme from municipal solid waste residue by two novel cellulolytic fungi. Biotech. Res. Internat. 2011:8. https://doi.org/10.4061/2011/810425 (Article ID 810425) CrossRefGoogle Scholar
- Moussa TAA, Tharwat NA (2013) Optimization of cellulase and glucosidase induction by sugarbeet pathogen Sclerotium rolfsii. Afr J Bot 1(4):44–49Google Scholar
- Nagasathya A, Sivanesan D, Ali EA (2014) Extracellular cellulase enzyme production from Penicillium funiculosum. Int J Adv Res Biol Sci 1(1):87–100Google Scholar
- Singh L, Bagri RK, Thakore BBL, Singh J, Sharma P (2012) Effect of carbon sources on production of enzyme by Fusarium oxysporm f. sp. pisi. Ann Pl Prot Sci 20(2):430–433Google Scholar