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Indian Phytopathology

, Volume 71, Issue 4, pp 599–610 | Cite as

Aggressiveness and genetic diversity of Ralstonia solanacearum strains from tomato in Vietnam

  • Hai Thi Hong TruongEmail author
  • Thanh-Thuy Duong
  • Tram Thi Hoai Nguyen
  • Thuy Thi Thu Nguyen
  • Linh Hoang Khanh Nguyen
  • Tho Thi Quynh Bui
Research Article
  • 10 Downloads

Abstract

In Vietnam, bacterial wilt, caused by R. solanacearum, is a major threat to many important field crops. Disease management strategies suggested in the past have remained ineffective. Phylotype analysis and genetic diversity among Vietnamese R. solanacearum is unknown. The present study was conducted to investigate the relationship among isolates of R. solanacearum in the tomato production areas in Vietnam, to analyze genotypic characteristics, to characterize the phylotypes of the isolates and to determine their aggressiveness. All 207 R. solanacearum strains collected from tomato plants in 12 provinces in Vietnam belonged to Asian Phylotype I. All isolates belong to biovar 3, except for isolate Rs86 collected from Ninh Thuan, which belonged to biovar 4. A total of 52 isolates representing 12 origins (provinces) and two Taiwanese strains were selected to investigate genetic diversity and their genetic relationship by using RAPD markers. Based on the unweighted pair group method of arithmetic means endrogram and STRUCTURE results, 54 isolates were grouped into three main clusters. Cluster I includes 3 isolates obtained from Lam Dong province and 1 isolate collected from Tien Giang province. Cluster II had wide distribution from the northern to the southern part of Vietnam. This cluster could be divided into 3 sub-clusters and these sub-clusters were largely separated according to geographical distribution. Sub-cluster IIa consisted of five isolates from Lam Dong province. One isolate from Thai Binh and two isolates from Hai Duong grouped in sub-cluster IIb. Cluster IIc was found only in Hai Phong. Cluster III had highest variation with two sub-clusters. Eight isolates from Southern part, 5 isolates from Nghe An and 2 reference isolates from Taiwan were grouped in sub-cluster IIIa, while 14 isolates mainly from Northern part (Ha Noi, Nam Dinh, and Thai Binh) were grouped in sub-cluster IIIb. Isolates collected in Hai Duong, Hai Phong and Nam Dinh showed higher virulence than those on tomato. The results demonstrated that R. solanacearum isolates even though similar in genetic background do not necessarily cause similar virulence. This is the first report on genetic diversity and aggressiveness of Vietnamese R. solanacearum isolates in tomatoes.

Keywords

Bacterial wilt Pathogenicity Aggressiveness Isolates Vietnam 

Notes

Acknowledgements

We are grateful to Vietnam National Foundation for Science and Technology Development (NAFOSTED) for supporting research Grant No 106-NN.99-2013.05. Many thanks to Dr. Jaw-Fen Wang for providing DNA of Taiwanese R. solanacearum strains.

Supplementary material

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Supplementary material 1 (DOCX 48 kb)
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Supplementary material 4 (DOC 71 kb)

References

  1. Buddenhagen I, Sequeira L, Kelman A (1962) Designation of races in Pseudomonas solanacearum (Abstr.). Phytopathology 52:726Google Scholar
  2. Chen Y, He L, Xu J (2005) Detection of bacterial wilt infection in potato using PCR. Acta Phytophylacica Sin 32(2):129–132Google Scholar
  3. Cook D, Sequeira L (1994) Strain differentiation of Pseudomonas solanacearum by molecular genetics methods. In: Hayward AC, Hartman GL (eds) Bacterial wilt: the disease and its causative agent, Pseudomonas solanacearum. CAB International, Wallingford, pp 77–93Google Scholar
  4. Cook D, Barlow E, Sequeira L (1989) Genetic diversity of Pseudomonas solanacearum: detection of restriction fragment length polymorphisms with DNA probes that specify virulence and hypersensitive response. Mol Plant Microbe Interact 2:113–121CrossRefGoogle Scholar
  5. Dinh PT, Do PT, Nguyen YT (2008) Genetic diversity of Pseudomonas solanacearum isolates caused bacterial wilt in peanut using RADP. J Sci Technol 46(6):43–50 (in Vietnamese) Google Scholar
  6. Do DT (2009) Commonality of bacterial wilt from different hosts in Ha Noi. J Plant Prod 2:38–42 (in Vietnamese) Google Scholar
  7. Doan TT (1995). Study on Pseudomonas solanacearum on different hosts in Northern Vietnam. Ph.D. Dissertation, Ha Noi, Vietnam, Ha Noi Agriculture University (in Vietnamese)Google Scholar
  8. Fegan M, Prior P (2005) How complex is the Ralstonia solanacearum species complex. In: Allen C, Prior P, Hayward AC (eds) Bacterial wilt disease and the Ralstonia solanacearum species complex, 1st edn. APS Press, MinnesotaGoogle Scholar
  9. Fegan M, Taghavi M, Sly LI, Hayward AC (1998) Phylogeny diversity and molecular diagnostics of Ralstonia solanacearum. In: Allen C, Prior P, Elphinstone J (eds) Bacterial wilt disease: molecular and ecological aspects. Springer, Berlin, pp 19–33CrossRefGoogle Scholar
  10. Fry WE (1978) Quantification of general resistance of potato cultivars and fungicide effects for integrated control of potato late blight. Phytopathology 68:1650–1655CrossRefGoogle Scholar
  11. Guidot A, Prior P, Schoenfeld J, Carrere S, Genin S, Boucher C (2007) Genomic structure and phylogeny of the plant pathogen Ralstonia solanacearum inferred from gene distribution analysis. J Bacteriol 189:377–387CrossRefGoogle Scholar
  12. Hayward AC (1964) Characteristics of Pseudomonas solanacearum. J Appl Bacteriol 27:265–277CrossRefGoogle Scholar
  13. Hayward AC (1991) Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu Rev Phytopathol 29:65–87CrossRefGoogle Scholar
  14. Hayward AC (1994) The hosts of Pseudomonas solanacearum. In: Hayward AC, Hartman GL (eds) Bacterial wilt: the disease and its causative agent, Pseudomonas solanacearum. CAB International, WallingfordGoogle Scholar
  15. Horita M, Tsuchiya K (2001) Genetic diversity of Japanese strains of Ralstonia solanacearum. Phytopathology 91:399–407CrossRefGoogle Scholar
  16. Ivey MLL, McP Garderner B, Opina N, Miller SA (2007) Diversity of Ralstonia solanacearum infecting eggplant in the Philippines. Phytopathology 97:1467–1475CrossRefGoogle Scholar
  17. James D, Girija D, Mathew SK, Nazeem PA, Balu TD, Varma SA (2003) Detection of Ralstonia solanacearum race 3 causing bacterial wilt of solanaceous vegetables in Kerala, using random amplified polymorphic DNA (RAPD) analysis. J Trop Agric 41:33–37Google Scholar
  18. Jeong YH, Kim J, Kang YS, Lee SD, Hwang IY (2007) Genetic diversity and distribution of Korean isolates of Ralstonia solanacearum. Plant Dis 91:1277–1287CrossRefGoogle Scholar
  19. Kelman A (1954) The relationship of pathogenicity of Pseudomonas solanacearumto colony appearance in Tetrazolium medium. Phytopathology 51:158–161Google Scholar
  20. Kumar A, Sarma YR, Anandaraj M (2004) Evaluation of genetic diversity of Ralstonia solanacearum causing bacterial wilt of ginger using REP–PCR and PCR–RFLP. Curr Sci 87:1555–1561Google Scholar
  21. Le TL (1997) Influence of different ecology factors on bacterial wilt in peanut in Northern Vietnam. J Plant Prod 4:5–8 (in Vietnamese) Google Scholar
  22. Lebeau A, Daunay MC, Frary A, Palloix A, Wang JF, Dintinger J, Chiroleu F, Wicker E, Prior P (2011) Bacterial wilt resistance in tomato, pepper, and eggplant: genetic resource respond to diverse strain in the Ralstonia solanacearum species complex. Phytopathology 101:154–165CrossRefGoogle Scholar
  23. Li X, Dorsch M, Del Dot T, Sly LI, Stackebrandt E, Hayward AC (1993) Phylogenetic studies of the rRNA group II pseudomonads based on 16S rRNA gene sequences. J Appl Bacteriol 74:324–329CrossRefGoogle Scholar
  24. Lin CH, Tsai KC, Prior P, Wang JF (2014) Phylogenetic relationship structure of Ralstonia solanacearum isolated from diverse origins in Taiwan. Plant Pathol 63:1395–1403CrossRefGoogle Scholar
  25. Nguyen TT (2012) Study on bacterial wilt caused by Ralstonia solanacearum smith in peanut and Potato in Ha Noi and control strategies. Ph.Dissertation, Ha Noi, Vietnam, Ha Noi Agriculture University (in Vietnamese)Google Scholar
  26. Nguyen LT, Phan TB (1993) Bacterial wilt in peanut in Northern Vietnam. In: Proceedings of plant production, pp 15–16 (in Vietnamese)Google Scholar
  27. Nguyen HX, Nguyen YT, Nguyen LV (1997) Results of study on distribution and damage of R. solanacearum in Nothern Vietnam. J Plant Prod 6:27–31 (in Vietnamese) Google Scholar
  28. Nguyen YT, Nguyen VV, Dang LTP (2002) Race and biovar of Ralstonia solanacearum caused bacterial wilt in different hosts. In: Proceeding of plant disease and biotechnology, Ha Noi, Vietnam, pp 64–66 (in Vietnamese)Google Scholar
  29. Opina N, Tavner F, Hollway G, Wang JF, Li TH, Maghirang R, Fegan M, Hayward AC, Krishnapillai V, Hong WF, Holloway BW, Timmis JN (1997) A novel method for development of species and strain-specific DNA probes and PCR primers for identifying Burkholderia solanacearum (Formerly Pseudomonas solanacearum). Asia Pac J Mol Biol Biotechnol 5:19–30Google Scholar
  30. Poussier S, Trigalet-Demery D, Vanderwalle P, Goffinet B, Luisetti J, Trigalet A (2000) Genetic diversity of Ralstonia solanacearum as assessed by PCR-RFLP of the hrp gene region, AFLP and 16S rRNA sequence analysis, and identification of an African subdivision. Microbiology 146:1679–1692CrossRefGoogle Scholar
  31. Prasannakumar MK, Chandrashekara KN, Deepa M, Vani A, Khan ANA (2012) Finger printing of Ralstoniasolanacearum isolates by Rep-PCR and RAPD. Pest Man Hortic Ecosyst 18:179–187Google Scholar
  32. Prior P, Fegan M (2005) Recent developments in the phylogeny and classification of Ralstonia solanacearum. Acta Hortic 695:127–136CrossRefGoogle Scholar
  33. Pritchard JK, Jonathan K, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155(2):945–959Google Scholar
  34. Rodrigues LMR, Destéfano SAL, da Silva MJ, Costa GGL, Maringoni AC (2012) Characterization of Ralstonia solanacearum strains from Brazil using molecular methods and pathogenicity tests. J Plant Pathol 94(3):505–516Google Scholar
  35. Sagar V, Gurjar MS, Arjunan J, Bakade RR, Chakrabarti SK, Arora RK, Sharma S (2014) Phylotype analysis of Ralstonia solanacearum strains causing potato bacterial wilt in Karnataka in India. Afr J Microbiol Res 8:1277–1281CrossRefGoogle Scholar
  36. Seal SE, Jackson LA, Daniels MJ (1992) Use of tRNA consensus primers to indicate subgroups of Pseudomonas solanacearum by polymerase chain reaction amplification. Appl Environ Microbiol 58:3759–3761Google Scholar
  37. Shaner G, Finney RE (1977) The effect of nitrogen fertilization in the expression of slow-mildewing resistance in Knox wheat. Phytopathology 67:1051–1056CrossRefGoogle Scholar
  38. Truong HTH, Wang JF, Esch E (2008) Resistance to Taiwanese race 1 strains of Ralstonia solanacearum in wild tomato germplasm. Eur J Plant Pathol 122:471–479CrossRefGoogle Scholar
  39. Truong HTH, Tran TV, Nguyen TTT, Pham BT, Tran HN (2015) Aggressiveness assessment of bacterial Ralstonia solanacearum isolates in the Nothern region. Sci Technol J Agric Rur Dev 260:19–25 (in Vietnamese) Google Scholar
  40. Tung PX, Vander Zaag P, Schemiediche P (1992) Effects of resistance genes, heat tolerance genes and cytoplasm on expression of resistance to Pseudomonas solanacearum in potato. Euphitica 60:127–138CrossRefGoogle Scholar
  41. Wang JF, Hanson PM, Barnes JA (1998) Worldwide evaluation of an international set of resistance sources to bacterial wilt in tomato. In: Prior P, Allen C, Elphinstone J (eds) Bacterial wilt disease: molecular and ecological aspects. Springer, Berlin, pp 269–275CrossRefGoogle Scholar
  42. Wang JF, Fang IH, Truong HTH, Huang SM, Balareto CH, Dittapongpitch V, Hidayati N (2013) Identification of major QTLs associated with stable resistance of tomato cultivar ‘Hawaii 7996’ to Ralstonia solanacearum. Euphytica 190:241–252CrossRefGoogle Scholar
  43. Wicker E, Lefeuvre P, Cambiaire JC, Lamaire C, Poussier S, Proir P (2012) Contrasting recombination patterns and demographic histories of the plant pathogen Ralstonia solanacearum interred from MLSA. Int Soc Microb Ecol J 6:1–14Google Scholar
  44. Winstead NN, Kelman A (1952) Inoculation techniques for evaluating resistance to Pseudomonas solanacearum. Phytopathology 42:628–634Google Scholar
  45. Xue QY, Yin YN, Yang W, Heuer H, Prior P, Guo JH, Smalla K (2011) Genetic diversity of Ralstonia solanacearum strains from China asessed by PCR-based fingerprints to unravel host plant- and site-dependent distribution patterns. FEMS Microbiol Ecol 75:507–519CrossRefGoogle Scholar

Copyright information

© Indian Phytopathological Society 2018

Authors and Affiliations

  1. 1.Institute of BiotechnologyHue UniversityPhu VangVietnam
  2. 2.Agronomy FacultyUniversity of Agriculture and Forestry, Hue UniversityHue CityVietnam
  3. 3.Faculty of Land Resources and Agricultural EnvironmentUniversity of Agriculture and Forestry, Hue UniversityHue CityVietnam
  4. 4.Ha Tinh UniversityHa Tinh CityVietnam

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