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Marker assisted introgression of a major bacterial blight resistance gene, Xa38 into a rice maintainer line, APMS 6B

  • A. Yugander
  • R. M. Sundaram
  • K. Singh
  • M. S. Prasad
  • A. S. Hari Prasad
  • M. S. Madhav
  • Gouri Sankar LahaEmail author
Research Article
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Abstract

Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae is a major production constraint for rice in India. We introgressed a major dominant BB resistance gene, Xa38 in the genetic background of APMS 6B, a maintainer line of APMS 6A (female parent of DRRH 3) through marker-assisted backcross breeding (MABB). The BB resistance gene, Xa38 was transferred to APMS 6B from the donor, PR 114 (Xa38) through two backcrosses. A single selected true BC2F1 plant having maximum recovery of recurrent parent genome and also high degree of BB resistance was selfed to generate BC2F2s. At BC2F2, plants homozygous for Xa38 were identified and were further confirmed for the absence of two major fertility restorer genes, Rf3 and Rf4. Eight best BC2F2 plants, homozygous positive for Xa38 and homozygous negative for Rf3 and Rf4 were selected and advanced up to BC2F6. At BC2F6, these introgression lines exhibited high degree of BB resistance against multiple Xoo strains and were similar to APMS 6B in respect to different agro-morphological traits.

Keywords

Bacterial blight of rice Hybrid rice Xanthomonas oryzae pv. oryzae Resistance Marker-assisted backcross breeding 

Notes

Acknowledgements

The authors thank the Director of ICAR-IIRR for providing the research facilities.

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Copyright information

© Indian Phytopathological Society 2019

Authors and Affiliations

  • A. Yugander
    • 1
    • 2
  • R. M. Sundaram
    • 1
  • K. Singh
    • 3
  • M. S. Prasad
    • 1
  • A. S. Hari Prasad
    • 1
  • M. S. Madhav
    • 1
  • Gouri Sankar Laha
    • 1
    Email author
  1. 1.ICAR-Indian Institute of Rice ResearchHyderabadIndia
  2. 2.Department of Plant SciencesUniversity of HyderabadHyderabadIndia
  3. 3.ICAR-National Bureau of Plant Genetic ResourcesNew DelhiIndia

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