Advertisement

Journal of Bionic Engineering

, Volume 16, Issue 5, pp 943–953 | Cite as

The Effects of Bio-inspired Electromagnetic Fields on Normal and Cancer Cells

  • Xuelei Liu
  • Zongming Liu
  • Zhenning LiuEmail author
  • Shujun ZhangEmail author
  • Kamal Bechkoum
  • Michael Clark
  • Luquan Ren
Article
  • 1 Downloads

Abstract

The electromagnetic field (EMF) is one of many environmental factors, which earth creatures are exposed to. There are many reports on the effects of EMF on living organisms. However, since the mechanism has not yet been fully understood, the biological effects of EMF are still controversial. In order to explore the effects of bio-inspired EMF (BIEMF) on normal and cancer cells, various cultured cells have been exposed to BIEMF of different directions, i.e. vertical, parallel and inclined. Significantly reduced ATP production in Hela and A549 cancer cells is found for the parallel and vertical BIEMF. More careful examination on Hela cells has revealed a cell density dependent inhibition on colony formation. The morphological observation of BIEMF-exposed Hela cells has suggested that the retarded cell proliferation is probably caused by cell death via apoptosis. Together these results may afford new insights for cancer prevention and treatment.

Keywords

electromagnetic fields (EMF) bio-inspired electromagnetic fields (BIEMF) directionality cancer cell proliferation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Notes

Acknowledgement

This work was supported by National Natural Science Foundation of China (51605188 and 51605187), the Joint Program of Jilin Province and Jilin University (SXGJQY2017-1 and SXGJSF2017-2), Jilin Provincial Science & Technology Department (20190303039SF), and Program for JLU Science and Technology Innovative Research Team (2017TD-04).

Supplementary material

42235_2019_108_MOESM1_ESM.pdf (953 kb)
The Effects of Bio-inspired Electromagnetic Fields on Normal and Cancer Cells

References

  1. [1]
    Cherry N. Schumann resonances, a plausible biophysical mechanism for the human health effects of solar/geomagnetic activity. Natural Hazards, 2002, 26, 279–331.CrossRefGoogle Scholar
  2. [2]
    Palmer S J, Rycroft M J, Cermack M. Solar and geomagnetic activity, extremely low frequency magnetic and electric fields and human health at the earth’s surface. Surveys in Geophysics, 2006, 27, 557–595.CrossRefGoogle Scholar
  3. [3]
    Fu J P, Mo W C, Liu Y, He R Q. Decline of cell viability and mitochondrial activity in mouse skeletal muscle cell in a hypomagnetic field. Bioelectromagnetics, 2016, 37, 212–222.CrossRefGoogle Scholar
  4. [4]
    Martino C F, Portelli L, McCabe K, Hernandez M, Barnes F. Reduction of the earth’s magnetic field inhibits growth rates of model cancer cell lines. Bioelectromagnetics, 2010, 31, 649–655.CrossRefGoogle Scholar
  5. [5]
    Galland P, Pazur A. Magnetoreception in plants. Journal of Plant Research, 2005, 118, 371–389.CrossRefGoogle Scholar
  6. [6]
    Pazur A, Schimek C, Galland P. Magnetoreception in microorganisms and fungi. Central European Journal of Biology, 2007, 2, 597–659.Google Scholar
  7. [7]
    Buchachenko A. Why magnetic and electromagnetic effects in biology are irreproducible and contradictory? Bioelectromagnetics, 2016, 37, 1–13.CrossRefGoogle Scholar
  8. [8]
    Grissom C B. Magnetic field effects in biology: A Survey of possible mechanisms with emphasis on radical-pair recombination. Chemical Reviews, 1995, 95, 3–24.CrossRefGoogle Scholar
  9. [9]
    Vijayalaxmi, Scarfi M R. International and national expert group evaluations: Biological/health effects of radiofrequency fields. International Journal of Environmental Research and Public Health, 2014, 11, 9376–9408.CrossRefGoogle Scholar
  10. [10]
    Hore P J. Are biochemical reactions affected by weak magnetic fields? Proceedings of the National Academy of Sciences of the United States of America, 2012, 109, 1357–1358.CrossRefGoogle Scholar
  11. [11]
    Naarala J, Kesari K K, Mcclure I, Chavarriaga C, Juutilainen J, Martino C F. Direction-dependent effects of combined static and ELF magnetic fields on cell proliferation and superoxide radical production. BioMed Research International, 2017, 2017, 5675086.CrossRefGoogle Scholar
  12. [12]
    Tian X F, Wang D M, Zha M, Yang X X, Ji X M, Zhang L, Zhang X. Magnetic field direction differentially impacts the growth of different cell types. Electromagnetic Biology & Medicine, 2018, 37, 114–125.CrossRefGoogle Scholar
  13. [13]
    Milovanovich I D, Ćirković S, De Luka S R, Djordjevich D M, Ilić A Z, Popović T, Arsić A, Obradović D D, Oprić D, Ristić-Djurović J L, Trbovich A M. Homogeneous static magnetic field of different orientation induces biological changes in subacutely exposed mice. Environmental Science & Pollution Research, 2016, 23, 1584–1597.CrossRefGoogle Scholar
  14. [14]
    Zimmerman J W, Pennison M J, Brezovich I, Yi N, Yang C T, Ramaker R, Absher D, Myers R M, Kuster N, Costa F P, Barbault A, Pasche B. Cancer cell proliferation is inhibited by specific modulation frequencies. British Journal of Cancer, 2012, 106, 307–313.CrossRefGoogle Scholar
  15. [15]
    Barbault A, Costa F P, Bottger B, Munden R F, Bomholt F, Kuseter N, Pasche B. Amplitude-modulated electromagnetic fields for the treatment of cancer: Discovery of tumor-specific frequencies and assessment of a novel therapeutic approach. Journal of Experimental Clinical Cancer Research, 2009, 28, 51.CrossRefGoogle Scholar
  16. [16]
    Costa F P, de Oliveira A C, Meirelles R, Machado M C, Zanesco T, Surjan R, Chammas M C, de Souza Rocha M, Morgan D, Cantor A, Zimmerman J, Brezovich I, Kuster N, Barbault A, Pasche B. Treatment of advanced hepatocellular carcinoma with very low levels of amplitude-modulated electromagnetic fields. British Journal of Cancer, 2011, 105, 640–648.CrossRefGoogle Scholar
  17. [17]
    Zimmerman J W, Jimenez H, Pennison M J, Brezovich I, Morgan D, Mudry A, Costa F P, Barbault A, Pasche B. Targeted treatment of cancer with radiofrequency electro magnetic fields amplitude-modulated at tumor-specific frequencies. Chinese Journal of Cancer, 2013, 32, 573–581.CrossRefGoogle Scholar
  18. [18]
    Kirson E D, Dbalý V, Tovarys F, Vymazal J, Soustiel J F, Itzhaki A, Mordechovich D, Steinberg-Shapira S, Gurvich Z, Schneiderman R, Wasserman Y, Salzberg M, Ryffel B, Goldsher D, Dekel E, Palti Y. Alternating electric fields arrest cell proliferation in animal tumor models and human brain tumors. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104, 10152–10157.CrossRefGoogle Scholar
  19. [19]
    Kirson E D, Gurvich Z, Schneiderman R, Dekel E, Itzhaki A, Wasserman Y, Schatzberger R, Palti Y. Disruption of cancer cell replication by alternating electric fields. Cancer Research, 2004, 64, 3288–3295.CrossRefGoogle Scholar
  20. [20]
    Davies A M, Weinberg U, Palti Y. Tumor treating fields: A new frontier in cancer therapy. Annals of the New York Academy of Sciences, 2013, 1291, 86–95.CrossRefGoogle Scholar
  21. [21]
    Kirson E D, Giladi M, Gurvich Z, Itzhaki A, Mordechovich D, Schneiderman R S, Wasserman Y, Ryffel B, Goldsher D, Palti Y. Alternating electric fields (TTFields) inhibit metastatic spread of solid tumors to the lungs. Clinical & Experimental Metastasis, 2009, 26, 633–640.CrossRefGoogle Scholar
  22. [22]
    Kirson E D, Schneiderman R S, Dbalý V, Tovaryš F, Vymazal J, Itzhaki A, Mordechovich D, Gurvich Z, Shmueli E, Goldsher D, Wasserman Y, Palti Y. Chemotherapeutic treatment efficacy and sensitivity are increased by adjuvant alternating electric fields (TTFields). BMC Medical Physics, 2009, 9, 1.CrossRefGoogle Scholar
  23. [23]
    Filipovic N, Djukic T, Radovic M, Cvetkovic D, Curcic M, Markovic S, Peulic A, Jeremic B. Electromagnetic field investigation on different cancer cell lines. Cancer Cell International, 2014, 14, 84.CrossRefGoogle Scholar
  24. [24]
    Buckner C A, Buckner A L, Koren S A, Persinger M A, Lafrenie R M. The effects of electromagnetic fields on B16-BL6 cells are dependent on their spatial and temporal character. Bioelectromagnetics, 2016, 38, 165–174.CrossRefGoogle Scholar
  25. [25]
    Meijer D K, Geesink H J. Favourable and unfavourable EMF frequency patterns in cancer: Perspectives for improved therapy and prevention. Journal of Cancer Therapy, 2018, 9, 188–230.CrossRefGoogle Scholar
  26. [26]
    Geltmeier A, Rinner B, Bade D, Meditz K, Witt R, Bicker U, Philipp C B, Maier P. Characterization of dynamic behaviour of MCF7 and MCF10A cells in ultrasonic field using modal and harmonic analyses. PLOS ONE, 2015, 10, e0134999.CrossRefGoogle Scholar
  27. [27]
    Nuccitelli R, Pliquett U, Chen X, Ford W, Swanson R J, Beebe S J, Kolb J F, Schoenbach K H. Nanosecond pulsed electric fields cause melanomas to self-destruct. Biochemical and Biophysical Research Communications, 2006, 343, 351–360.CrossRefGoogle Scholar
  28. [28]
    Novikov V V, Ponomarev V O, Novikov G V, Kuvichkin V V, Iablokova E V, Fesenko E E. Effects and molecular mechanisms of the biological action of weak and extremely weak magnetic fields. Biofizika, 2010, 55, 631–639.Google Scholar
  29. [29]
    Zhadin M N. Review of Russian literature on biological action of DC and low-frequency AC magnetic fields. Bioelectromagnetics, 2015, 22, 27–45.CrossRefGoogle Scholar
  30. [30]
    Knowles J R. Enzyme-catalyzed phosphoryl transfer reactions. Annual Review of Biochemistry, 1980, 49, 877–919.CrossRefGoogle Scholar
  31. [31]
    Buchachenko A L, Kuznetsov D A. Magnetic field affects enzymatic ATP synthesis. Journal of the American Chemical Society, 2008, 130, 12868–12869.CrossRefGoogle Scholar
  32. [32]
    Shi Z, Yu H, Sun Y, Yang C, Lian H, Cai P. The energy metabolism in caenorhabditis elegans under the extremely low-frequency electromagnetic field exposure. Scientific Reports, 2015, 5, 8471.CrossRefGoogle Scholar
  33. [33]
    Buchachenko A L, Kouznetsov D A, Orlova M A, Markarian A A. Magnetic isotope effect of magnesium in phosphoglycerate kinase phosphorylation. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102, 10793–10796.CrossRefGoogle Scholar
  34. [34]
    Hore P J, Mouritsen H. The radical-pair mechanism of magnetoreception. Annual Review of Biophysics, 2016, 45, 299–344.CrossRefGoogle Scholar
  35. [35]
    Kirschvink J L, Gould J L. Biogenic magnetite as a basis for magnetic field detection in animals. Biosystems, 1981, 13, 181–201.CrossRefGoogle Scholar
  36. [36]
    Shcherbakov V P, Winklhofer M. The osmotic magnetometer: A new model for magnetite-based magnetoreceptors in animals. European Biophysics Journal, 1999, 28, 380–392.CrossRefGoogle Scholar
  37. [37]
    Davila A F, Winklhofer M, Shcherbakov V P, Petersen N. Magnetic pulse affects a putative magnetoreceptor mechanism. Biophysical Journal, 2005, 89, 56–63.CrossRefGoogle Scholar
  38. [38]
    Fleissner G, Stahl B, Thalau P, Falkenberg G, Fleissner G. A novel concept of Fe-mineral-based magnetoreception: Histological and physicochemical data from the upper beak of homing pigeons. Naturwissenschaften, 2007, 94, 631–642.CrossRefGoogle Scholar
  39. [39]
    Liboff A R, Jenrow K A. New model for the avian magnetic compass. Bioelectromagnetics, 2000, 21, 555–565.CrossRefGoogle Scholar
  40. [40]
    Buchachenko A L, Kouznetsov D A, Breslavskaya N N, Orlova M A. Magnesium isotope effects in enzymatic phosphorylation. Journal of Physical Chemistry B, 2008, 112, 2548–2556.CrossRefGoogle Scholar
  41. [41]
    Mo W C, Zhang Z J, Liu Y, Bartlett P F, He R Q. Magnetic shielding accelerates the proliferation of human neuroblastoma cell by promoting G1-Phase progression. PLOS ONE, 2013, 8, e54775.CrossRefGoogle Scholar
  42. [42]
    Wong D W, Gan W L, Teo Y K, Lew W S. Interplay of cell death signaling pathways mediated by alternating magnetic field gradient. Cell Death Discovery, 2018, 4, 49.CrossRefGoogle Scholar
  43. [43]
    Kroemer G, El-Deiry W S, Golstein P, Peter M E, Vaux D, Vandenabeele P, Zhivotovsky B, Bla-gosklonny M V, Malorni W, Knight R A, Piacentini M, Nagata S, Melino G. Classification of cell death: Recommendations of the nomenclature committee on cell death. Cell Death & Differentiation, 2005, 2, 1463–1467.CrossRefGoogle Scholar
  44. [44]
    Krysko D V, Berghe T V, Katharina D’Herde, Vandenabeele P. Apoptosis and necrosis: Detection, discrimination and phagocytosis. Methods, 2008, 44, 205–221.CrossRefGoogle Scholar

Copyright information

© Jilin University 2019

Authors and Affiliations

  • Xuelei Liu
    • 1
  • Zongming Liu
    • 1
    • 2
  • Zhenning Liu
    • 1
    Email author
  • Shujun Zhang
    • 1
    • 3
    Email author
  • Kamal Bechkoum
    • 3
  • Michael Clark
    • 4
  • Luquan Ren
    • 1
  1. 1.Key Laboratory of Bionic Engineering (Ministry of Education), College of Biological and Agricultural EngineeringJilin UniversityChangchunChina
  2. 2.Department of AnesthesiologyJilin Cancer HospitalChangchunChina
  3. 3.School of Computing and TechnologyUniversity of Gloucestershire, The ParkCheltenhamUK
  4. 4.Magnacare Health GroupBirminghamUK

Personalised recommendations