Monilinia fructicola and M. laxa, causing brown rot in stone fruit (Prunus species), were first described from Western Australia in 1997. Our previous work indicated that original invasive isolates of both species were each of a single genotype. This research aims to compare isolates from the region in the present day in terms of genetic diversity, colony morphology, and virulence on fruit. Genetic diversity of 68 M. laxa and 66 M. fructicola isolates collected in 2016-2017 from five populations was measured using ISSR markers. The genetic diversity within M. fructicola was greater than that of M. laxa, having Shannon’s diversity indices of 0.50 and 0.41, respectively. Most genetic variation was present within populations for both M. fructicola (77%) and M. laxa (76%), with the rest of the variation between geographically separated populations. Genotype did not correlate closely with virulence of isolates as measured by symptom severity index (SSI) on inoculated plum fruit and colony morphology on potato dextrose agar (PDA) medium. M. fructicola isolates with wide genetic variation exhibited closely similar SSIs and one morphotype on PDA, whereas M. laxa isolates exhibited a broad range of SSIs and four different morphotypes.
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Abate D, Pastore C, Gerin D, De Miccolis Angelini RM, Rotolo C, Pollastro S, Faretra F (2018a) Characterization of Monilinia spp. populations on stone fruit in South Italy. Plant Dis 102:1708–1717
Abate D, De Miccolis Angelini RM, Rotolo C, Pollastro S, Faretra F (2018b) Mating system in the brown rot pathogens Monilinia fructicola, M. laxa, and M. fructigena. Phyto 108:1315–1325
AMRiN (2015) Occurrence record: WAC - WAC9462 Monilinia. Department of Agriculture and Food - Western Australia. http://amrin.ala.org.au/occurrences/e656aaee-e0c4-4330-ab2d-eb15023f876a;jsessionid=4F6F18291AFA044E7FCB9C2AFFF8A7DC. Accessed 20 June 2019
Angeli SS, Mio LL, Amorim L (2017) Comparative analysis of Monilinia fructicola and M. laxa isolates from Brazil: monocyclic components of peach brown rot. Cienc Rural 47. https://doi.org/10.1590/0103-8478cr20160300
Bannon F, Gort G, van Leeuwen G, Holb I, Jeger M (2009) Diurnal patterns in dispersal of Monilinia fructigena conidia in an apple orchard in relation to weather factors. Agric For Meteorol 149:518–525
Biosecurity Australia (2006) Final Report: Pest risk analysis for stone fruit from New Zealand into Western Australia. Biosecurity Australia, Agriculture, Fishery and Forestry - Australia (AFFA). https://doi.org/10.1093/eurrag/29.3.329
Boothroyd A (2013) Imported US stone fruit approved for Aussie supermarkets. Food Beverage. 29 July. https://foodmag.com.au/imported-us-stone-fruit-approved-for-aussie-supermarkets/. Accessed 11 Apr 2017
Bosshard EH, Hilber-Bodmer M, Schärer HJ, Bünter M, Duffy B (2006) First report of the quarantine brown rot pathogen Monilinia fructicola on imported stone fruits in Switzerland. Plant Dis 90:1554
Cote MJ, Tardif MC, Meldrum AJ (2004) Identification of Monilinia fructigena, M.fructicola, M.laxa, and Monilia polystroma on inoculated and naturally infected fruit using multiplex PCR. Plant Dis 88:1219–1225
Cox KD, Scherm H (2001) Gradients of primary and secondary infection by Monilinia vaccinii-corymbosi from point sources of ascospores and conidia. Plant Dis 85:955–959
De Cal A, Gell I, Usall J, Vinas I, Melgarejo P (2009) First report of brown rot caused by Monilinia fructicola in peach orchards in Ebro Valley, Spain. Plant Dis 93:763–763
Duchoslavová J, Širučková I, Zapletalová E, Navratil M, Šafářová D (2007) First report of brown rot caused by Monilinia fructicola on various stone and pome fruits in the Czech Republic. Plant Dis 91:907
European and Mediterranean Plant Protection Organization (EPPO) (2019) https://gd.eppo.int/search?k=Monilia+fructicola
Fan JY, Guo LY, Xu JP, Luo Y, Michailides TJ (2010) Genetic diversity of populations of Monilinia fructicola (Fungi, Ascomycota, Helotiales) from China. J Eur Microbiol 57:206–212
Fazekas M, Madar A, Sipiczki M, Miklós I, Holb IJ (2014) Genetic diversity in Monilinia laxa populations in stone fruit species in Hungary. World J Microb Biot 30:1879–1892
Gell I, Cubero J, Melgarejo P (2007a) Two different PCR approaches for universal diagnosis of brown rot and identification of Monilinia spp. in stone fruit trees. J Appl Microbiol 103:2629–2637
Gell I, Larena I, Melgarejo P (2007b) Genetic diversity in Monilinia laxa populations in peach orchards in Spain. J Phytopathol 155:549–556
Grabke A, Hu MJ, Luo CX, Bryson PK, Schnabel G (2011) First report of brown rot of apple caused by Monilinia fructicola in Germany. Plant Dis 95:772
Harrison TH (1928) Brown rot of fruits and associated diseases in Australia. I. History of the disease and determination of the causal organism. Journal of the Proceedings of the Royal Society of New South Wales 52:99–148
Hewitt WB, Leach LD (1939) Brown rot Sclerotinias occurring in California and their distribution on stone fruits. Phytopathol 29:337–351
Holmes R, Kreidl S, Villalta O, Gouk C (2011) Through chain approach for managing brown rot in summerfruit and canning fruit. Project code MT08039. Biosciences research division, Department of Primary Industries, Victoria, Australia
Hort Innovation (2019) Australian horticulture statistics handbook – fruit. Horticulture Innovation Australia Ltd, Sydney, 231 pp
Hrustić J, Mihajlović M, Tanović B, Delibašić G, Stanković I, Krstić B, Bulajić A (2013) First report of brown rot caused by Monilinia fructicola on nectarine in Serbia. Plant Dis 97:147
Kable PF (1965) Air dispersal of conidia of Monilinia fructicola in peach orchards. Anim Prod Sci 5:166–171
Lane CR (2002) A synoptic key for differentiation of Monilinia fructicola, M. fructigena and M. Iaxa, based on examination of cultural characters. Bulletin OEPP 32:489–493
Lim S, Notley-McRobb L, Lim M, Carter DA (2004) A comparison of nature and abundance of microsatellite in 14 fungal genomes. Fungal Genet Biol 41:1025–1036
Luo CX, Schnabel G (2008) The cytochrome P450 lanosterol 14α-demethylase gene is a demethylation inhibitor fungicide resistance determinant in Monilinia fructicola field isolates from Georgia. Appl Environ Microbiol 74:359–366
Ma Z, Luo Y, Michailide TJ (2003) Nested PCR assays for detection of Monilinia fructicola in stone fruit orchards and Botryosphaeria dothidea from pistachios in California. J Phytopathol 151:312–322
Martini C, Lantos A, Di Francesco A, Guidareli M, D’Aquino S, Baraldi E (2014) First report of Asiatic brown rot caused by Monilinia polystroma on peach in Italy. Plant Dis 98:1585
Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590
Papavasileiou A, Karaoglanidis GS, Michailides TJ (2015) Intraspecific diversity of Monilinia fructicola and M. laxa populations from blossoms and fruit of different hosts in Greece. Plant Dis 99:1353–1359
Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in excel. Population genetic software for teaching and research – an update. Bioinformatics 28:2537–2539
Pellegrino C, Gullino ML, Garibaldi A, Spadaro D (2009) First report of brown rot of stone fruit caused by Monilinia fructicola in Italy. Plant Dis 93:668–668
Petroczy M, Palkovics L (2006) First report of brown rot caused by Monilinia fructicola on imported peach in Hungary. Plant Dis 90:375
Petróczy M, Palkovics L (2009) First report of Monilia polystroma on apple in Hungary. Eur J Plant Pathol 125:343–347
Poniatowska A, Michalecka M, Bielenin A (2013) Characteristic of Monilinia spp. fungi causing brown rot of pome and stone fruits in Poland. Eur J Plant Pathol 135:855–865
Rungjindamai N, Jeffries P, Xu XM (2014) Epidemiology and management of brown rot on stone fruit caused by Monilinia laxa. Eur J Plant Pathol 140:1–17
Shannon CE, Weaver W (1949) The mathematical theory of information. Illinois University Press, Urbana
Tran TT, Li H, Nguyen DQ, Sivasithamparam K, Jones MGK, Wylie SJ (2017) Spatial distribution of Monilinia fructicola and M. laxa in stone fruit production areas in Western Australia. Austral Plant Pathol 46:339–349
Tran TT, Li H, Nguyen DQ, Sivasithamparam K, Jones MGK, Wylie SJ (2018) Monilinia fructicola and Monilinia laxa isolates from stone fruit orchards sprayed with fungicides displayed a broader range of responses to fungicides than those from unsprayed orchards. Eur J Plant Pathol 153:985–999
Tran TT, Li H, Nguyen DQ, Sivasithamparam K, Jones MGK, Wylie SJ (2019) Genotypic structure of Monilinia populations in Western Australia two decades after incursion. Austral Plant Pathol 48:167–178
Van Leeuwen GC, Yen RPB, Holb IJ, Jeger MJ (2002) Distinction of the Asiatic brown rot fungus Monilia polystroma sp. nov. from M. fructigena. Mycol Res 106:444–451
Villarino M, Larena I, Martinez F, Melgarejo P, De Cal A (2012) Analysis of genetic diversity in Monilinia fructicola from the Ebro Valley in Spain using ISSR and RAPD markers. Eur J Plant Pathol 132:511–524
Willetts HJ, Bullock S (2019) I. cytology, histology, and histochemistry of fruit infection by Monilinia species. Cytology, Histology and Histochemistry of Fruit Tree Diseases
Wilson EE, Baker GA (1946) Some aspects of the aerial dissemination of spores, with special reference to conidia of Sclerotinia laxa. J Agr Res 72:30–327
Yin, L.F., Chen, S.N., Chen, G.K., Schnabel, G., Du, S.F., Chen, C., Li, G.Q. and Luo, C.X., 2015. Identification and characterization of three Monilinia species from plum in China. Plant disease, 99(12), pp.1775-1783.
Zhu XQ, Guo LY (2010) First report of brown rot on plum caused by Monilia polystroma in China. Plant Dis 94:478
Zhu XQ, Chen XY, Luo Y, Guo LY (2005) First report of Monilinia fructicola on peach and nectarine in China. Plant Pathol 54:575
Tran TT and Nguyen DQ each received a scholarship provided jointly by Vietnam International Education Development (VIED) and Murdoch University.
This article does not contain any studies with human participants or animals performed by any of the authors.
All fungal samples described here were collected with the fully informed consent of landowners.
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Tran, T.T., Li, H., Nguyen, D.Q. et al. Comparisons between genetic diversity, virulence and colony morphology of Monilinia fructicola and Monilinia laxa isolates. J Plant Pathol (2020). https://doi.org/10.1007/s42161-020-00498-2
- Brown rot
- ISSR markers
- Stone fruit