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Comparisons between genetic diversity, virulence and colony morphology of Monilinia fructicola and Monilinia laxa isolates

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Abstract

Monilinia fructicola and M. laxa, causing brown rot in stone fruit (Prunus species), were first described from Western Australia in 1997. Our previous work indicated that original invasive isolates of both species were each of a single genotype. This research aims to compare isolates from the region in the present day in terms of genetic diversity, colony morphology, and virulence on fruit. Genetic diversity of 68 M. laxa and 66 M. fructicola isolates collected in 2016-2017 from five populations was measured using ISSR markers. The genetic diversity within M. fructicola was greater than that of M. laxa, having Shannon’s diversity indices of 0.50 and 0.41, respectively. Most genetic variation was present within populations for both M. fructicola (77%) and M. laxa (76%), with the rest of the variation between geographically separated populations. Genotype did not correlate closely with virulence of isolates as measured by symptom severity index (SSI) on inoculated plum fruit and colony morphology on potato dextrose agar (PDA) medium. M. fructicola isolates with wide genetic variation exhibited closely similar SSIs and one morphotype on PDA, whereas M. laxa isolates exhibited a broad range of SSIs and four different morphotypes.

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Acknowledgements

Tran TT and Nguyen DQ each received a scholarship provided jointly by Vietnam International Education Development (VIED) and Murdoch University.

Author information

Correspondence to Stephen John Wylie.

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This article does not contain any studies with human participants or animals performed by any of the authors.

All fungal samples described here were collected with the fully informed consent of landowners.

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The authors declare no conflicts of interest, financial or non-financial. No funding bodies contributed to this research.

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Tran, T.T., Li, H., Nguyen, D.Q. et al. Comparisons between genetic diversity, virulence and colony morphology of Monilinia fructicola and Monilinia laxa isolates. J Plant Pathol (2020). https://doi.org/10.1007/s42161-020-00498-2

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Keywords

  • Brown rot
  • ISSR markers
  • Pathogenicity
  • Stone fruit