Advertisement

First report of ‘Candidatus Phytoplasma trifolii’ (16SrVI group) infecting cabbage (Brassica oleracea) in Turkey

  • Çiğdem Ulubaş SerçeEmail author
  • Salih Yılmaz
Disease Note
  • 166 Downloads

Keywords

Phytoplasma Cabbage ITS region SecA gene PCR 

Brassica oleracea is an economically important vegetable species. It is a member of the Brassicaceae family and widely cultivated worldwide as well as in Turkey. During late summer and autumn seasons of 2014 and 2015, a total of 331 cabbage samples exhibiting phytoplasma-like symptoms, such as deformation of leaves, abnormal coloration, stunting and failure to head were collected from in Niğde province located in Central Anatolia Region of Turkey. Total nucleic acids were extracted from leaf midribs of plants using the total nucleic acid isolation protocol (Prince et al. 1993). For initial screening of the samples, double nested PCR method was applied. 16SrRNA gene of DNA samples was amplified by direct PCR using P1/P7, followed by two sequential nested PCRs using R16F1/B6 and M1/M2 primer pairs, respectively. Out of 331, 29 of the samples revealed the expected ~500 bp amplicons after the last nested PCR with M1/M2 primer pair. In order to sequence bigger portion of the genome, the P1/P7 products were amplified with R16F2n/R16R2 primer pair. The obtained 1132 bp sequence belonged to TR-Lah19 isolate (GenBank accession No. MK392369) and had 99% identity with ‘Candidatus Phytoplasma trifolii’ isolate from tomato plant (KY047614). Non ribosomal secA gene of the same isolate was amplified using SecAfor1/SecArev3 primers. Sequenced 772 bp nucleotides (MK392370) revealed 98% identity with previously published secA gene sequences of ‘Ca. P. trifolii’ (KX784498) species belonging to the clover proliferation (CP) group (16Sr-VI). The CP group of phytoplasma infections in cabbage was previously reported in Iran and India (Salehi et al. 2007; Khasa et al. 2018). To best our knowledge this is the first report of CP group phytoplasma infection of cabbage in Turkey.

Notes

Funding information

This study was partially funded by Doğuş Tarımsal Projeler Ar-Ge Hizmetleri A.Ş.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Khasa E, Rao A, Rao GP (2018) Molecular characterization of ‘clover proliferation’phytoplasma subgroup-D (16SrVI-D) associated with vegetables crops in India. Physiol Mol Biol Plants 24(2):203–210CrossRefPubMedGoogle Scholar
  2. Prince JP, Davis RE, Wolf TK, Lee IM, Mogen BD, Dally EL, Bertaccini A, Credi R, Barba M (1993) Molecular detection of diverse mycoplasma like organisms (MLOs) associated with grapevine yellows and their classification with aster yellows, X-disease, and elm yellows MLOs. Phytopathology 83:1130–1137CrossRefGoogle Scholar
  3. Salehi M, Izadpanah K, Siampour M (2007) Characterization of a phytoplasma associated with cabbage yellows in Iran. Plant Dis 91:625–630CrossRefGoogle Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Department of Plant Production and Technologies, Ayhan Şahenk Faculty of Agricultural Sciences and TechnologiesNiğde Ömer Halisdemir UniversityNiğdeTurkey

Personalised recommendations