Detection of the race of Exserohilum turcicum [(Pass.) K.J. Leonard & Suggs] causing northern leaf blight diseases of corn in Turkey

  • Emine Burcu Turgay
  • Orhan Büyük
  • Berna TunalıEmail author
  • Özlem Helvacıoğlu
  • Şener Kurt
Original Article


Northern Leaf Blight caused by Exserohilum turcicum is the most significant disease in corn growing fields. The most important strategy for controlling Northern Leaf Blight is to use resistant varieties derived from qualitative and quantitative traits of corn grown around the world. Northern Leaf Blight threats to corn production because of the presence of E. turcicum races and new races potential. Therefore, in order to obtain and use resistant lines at the regional level, it is necessary to determine pathogen races. For this reason, the objective of this study was to identify races of 50 E. turcicum isolates collected by the survey carried out on 7 important corn producing areas, Sakarya, Samsun, Ordu, Adana, Mardin, Osmaniye, and Mersin provinces, belonging to the Marmara, Black Sea, Mediterranean and Southeastern Anatolia regions of Turkey. In result, 8 different races of northern leaf blight, namely 0, 1, 2, 123, N, 1 N, 3 N, 12 N were found in Turkey. The most common races were identified as race 0 and race 1. In addition, molecular identification studies confirmed the validity of morphologically identified E. turcicum isolates.


Northern leaf blight Exserohilum turcicum Race PCR 



This work was funded by the Scientific and Technological Research Council of Turkey (TÜBİTAK).


  1. Abebe D, Singburaudom N (2006) Morphological, cultural and pathogenicity variation of Exserohilum turcicum (Pass) Leonard and Suggs isolates in maize (Zea mays L.). Kasetsart J (Nat Sci) 40:341–352Google Scholar
  2. Bergquist R, Macias OR (1974) Physiologic specialization in Trichometasphaeria turcicum f. sp. zeae and and T. turcicum sp. sorghi in Hawaii. Phytopathology 83:612–614Google Scholar
  3. Cardwell KF, Schulthess F, Namah R, Ngoko Z (1997) A systems approach to assess crop health and maize yield losses due to pests and diseases in Cameroon. Agric Ecosyst Environ 65:33–47CrossRefGoogle Scholar
  4. Carson ML (1995) Inheritance of latent period length in maize infected with Exserohilum turcicum. Plant Dis 79:581–585CrossRefGoogle Scholar
  5. Chung CL, Longfellow JM, Walsh EK, Kerdieh Z, Van Esbroeck G, Balint-Kurti P, Nelson RJ (2010) Resistance loci affecting distinct stages of fungal pathogenesis: use of introgression lines for QTL mapping and characterization in the maize—Setosphaeria turcica pathosystem. BMC Plant Biol 10:103 35–237. 14CrossRefGoogle Scholar
  6. Dong J, Fan Y, Gui X, An X, Ma J, Dong Z (2008) Geographic distribution and genetic analysis of physiological races of Setosphaeria turcica in northern China. Am J Agric Biol Sci 3:389–398CrossRefGoogle Scholar
  7. Ellis MB (1971) Dematiaceous Hyphomycetes. Surrey, Commonwealth Mycological Institute 608pGoogle Scholar
  8. Fallah MP, Pataky JK (1994) Reactions of isolate from mating of races 1 and 23 N of Exserohilum turcicum. Plant Dis 78:767–771CrossRefGoogle Scholar
  9. Ferguson LM, Carson ML (2004) Spatial diversity of Setosphaeria turcica sampled from the eastern United States. Phytopathology 94:892–900CrossRefGoogle Scholar
  10. Gevers H (1975) A new major gene for resistance to. Helminthosporium turcicum leaf blight of maize. Plant Dis Rep 59:296–299Google Scholar
  11. Gianasi L, Castro HA de and de Silva HP (1996) Raças fisiológicas de Exserohilum turcicum identificadas em regiões produtoras de milho no Brasil, Safra 93/94. Summa Phytopathol 22:214–217Google Scholar
  12. Hilu HM, Hooker AL (1963) Monogenic chlorotic-lesion resistance to Helminthosporium turcicum in corn seedlings. Phytopathology 53:909–912Google Scholar
  13. Hooker AL (1977) A second major gene locus in corn for chlorotic-lesion resistance to Helminthosporium turcicum. Crop Sci 17:132–135CrossRefGoogle Scholar
  14. Hooker AL (1981) Resistance to Helminthosporium turcicum from Tripsacum floridanum incorporated into corn. Maize Genet Coop News Lett 55:87–88Google Scholar
  15. Leonard KJ, Levy Y, Smith DR (1989) Proposed nomenclature for pathogenic races of Exserohilum turcicum on corn. Plant Dis 73:776–777Google Scholar
  16. Muiru WM, Koopmann B, Tiedemann AV, Mutitu EW, Kimenju JW (2010) Race typing and evaluation of aggressiveness of Exserohilum turcicum isolates of Kenya, German and Austrian origin. World J Agric Sci 6(3):277–284Google Scholar
  17. Nwanosike MR, Mabagala R, Kusolwa PM (2015) Effect of northern leaf blight (Exserohilum turcicum) severity on yield of maize (Zea mays L.) in Morogoro, Tanzania. Int J Sci Res 4(9):466–475Google Scholar
  18. Ogliari JB, Guimaraes MA, Geraldi IO, Camargo LEA (2005) New resistance genes in the Zea mays - Exserohilum turcicum pathosystem. Genet Mol Biol 28:435–439CrossRefGoogle Scholar
  19. Pant SK, Kumar P, Chauhan VS (2000) Effect of Turcicum leaf blight on photosynthesis in maize. Indian Phytopathology 54:251–252Google Scholar
  20. Ramathani I, Biruma M, Martin T, Dixelius C, Okori P (2011) Disease severity incidence and races of Setosphaeria turcica on sorghum in Uganda. Eur J Plant Pathol 131:383–392CrossRefGoogle Scholar
  21. Raymundo AD, Hooker AL (1982) Single and combined effects of monogenic and polygenic resistance on certain components of northern corn leaf blight development. Phytopathology 72:99–103CrossRefGoogle Scholar
  22. Robbins WA, Warren HL (1993) Inheritance resistance to. Exserohilum turcicum in PI 209135, “Mayorbela” variety of mayze. Maydica 38:209–213Google Scholar
  23. Shekhar M., Kumar S. (2012) Inocularion methods and disease rating scales for maize diseases, 17–19. Directorate of Maize Research, Indian Council for Agricultural Research, New Delhi, IndiaGoogle Scholar
  24. Sibiya J, Tongoona P, Derera J, Makanda I (2013) Smallholder farmers’ perceptions of maize diseases, pests, and other production constraints, their implications for maize breeding and evaluation of local maize cultivars in Kwa Zulu- Natal, South Africa. Afr J Agric Res (17):1790–1798Google Scholar
  25. Simcox KD, Bennetzen JL (1993) The use of molecular markers to study Setosphaeria turcica resistance in maize. Phytopathology 83:1326–1330CrossRefGoogle Scholar
  26. Smith DR, Kinsey JG (1980) Further physiologic specialization in Helminthosporium turcicum. Plant Dis 64:779–781CrossRefGoogle Scholar
  27. Thakur RP, Leonard KJ, Jones RK (1989) Characterization of a new race of Exserohilum turcicum virulent on corn with resistance gene HtN. Plant Dis 73:151–155CrossRefGoogle Scholar
  28. Turgay EB, Büyük O, Tunalı B, Kurt Ș, Akçali E, Baran B, Helvacıoğlu Ö, Engİnsu S, Kansu B (2017) The spread of northern [Exserohilum turcicum (Pass.) K.J. Leonard & Suggs] and southern [Bipolaris maydis (Y.Nisk. & C. Miyake) shoemaker] leaf blight diseases in main corn (Zea mays L.) production areas in Turkey. Plant Protect Bull 57:357–372Google Scholar
  29. Turner MT, Johnson ER (1980) Race of Helminthosporium turcicum not controlled by Ht genetic resistance in corn in the American corn belt. Plant Dis 64:216–217CrossRefGoogle Scholar
  30. Weems JD (2016) Evaluation of race population distribution, fungicide sensitivity, and fungicide control of Exserohilum turcicum, The Causal Agent of Northern Leaf Blight of Corn. Ph.D. Thesis. Crop Sciences in the Graduate College of the University of Illinois at Urbana USAGoogle Scholar
  31. Welz HG (1998) Genetics and epidemiology of the pathosystem Zea mays/Setosphaeria turcica. Habilitation Thesis. Univ. of Hohenheim, Shaker-Verlag, Stuttgart/Aachen, GermanGoogle Scholar
  32. Welz HG, Geiger HH (2000) Genes for resistance to northern corn leaf blight in diverse maize populations. Plant Breed 119:1–14CrossRefGoogle Scholar
  33. Windes JM, Pedersen WL (1991) An isolate of Exserohilum turcicum virulent on maize inbreds with resistance gene. HtN Plant Dis 75:430CrossRefGoogle Scholar
  34. Wise K, Mueller D (2011) Are fungicides no longer just for fungi? An analysis of foliar fungicide use in corn. APS net feature article.
  35. Yeshitila D (2003) Cloning and characterization of xylanase genes from phytopathogenic fungi with a special reference to Helminthosporium turcicum the cause of northern leaf blight of maize. Ph.D. Thesis. Department of Applied Biology. University Of Helsinki. FinlandGoogle Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Department of Breeding and Genetics, Diseases and Pest ResistanceField Crops Central Research InstituteAnkaraTurkey
  2. 2.Plant Protection Central Reseach InstituteAnkaraTurkey
  3. 3.Department of Plant Protection, Faculty of AgricultureOndokuz Mayıs UniversitySamsunTurkey
  4. 4.Directorate of Plant Protection Research InstituteİzmirTurkey
  5. 5.Department of Plant Protection Faculty of AgricultureMustafa Kemal UniversityHatayTurkey

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