Advertisement

Efficacy of native antagonistic rhizobacteria in the biological control of Pythium aphanidermatum-induced damping-off of cucumber in Oman

  • Dhuha Sulaiman Salim Al-Daghari
  • Shurooq Abdullah Al-Abri
  • Issa Hashil Al-Mahmooli
  • Abdullah Mohammed Al-Sadi
  • Rethinasamy VelazhahanEmail author
Short Communication
  • 5 Downloads

Abstract

The efficacy of bacterial isolates obtained from the rhizosphere soil samples collected from cucumber fields of Muscat Governorate of Oman was assessed against Pythium aphanidermatum, the causal agent of damping-off of cucumber. Of the 58 isolates screened in a dual culture assay, only four isolates (B11, B9, AT3 and 4A) inhibited the growth of P. aphanidermatum and produced inhibition zones of above 5 mm. Among the four isolates, B11 was the most effective followed by B9, AT3 and 4A, which recorded inhibition zones of 14 mm, 13 mm, 11 mm and 6.8 mm, respectively. Scanning electron micrographs of hyphae of P. aphanidermatum grown in the presence of antagonists showed morphological abnormalities. These antagonistic bacteria were identified based on sequence analysis of 16S rDNA gene. The potential of the promising antagonists, Pseudomonas resinovorans (B11) and Pseudomonas aeruginosa (AT3) was evaluated either individually or in combination with a commercial formulation of Trichoderma viride against damping-off of cucumber under greenhouse conditions. Among the biocontrol treatments, soil application of P. resinovorans (B11) was the most effective, which resulted in a significantly lower damping-off incidence (24 to 30%) compared with Pythium-infected control (82 to 84%). To our knowledge, this is the first report on biological control of P. aphanidermatum with P. resinovorans.

Keywords

Biological control Cucumis sativus Damping-off Pseudomonas resinovorans Pythium aphanidermatum 

Notes

Acknowledgements

This study was supported by a grant (IG/AGR/CROP/18/01) from Sultan Qaboos University, Muscat, Oman. We thank Dr. Myo Tay Zar Myint, Department of Physics, College of Science, Sultan Qaboos University for his help in scanning electron microscopy.

Compliance with ethical standards

Ethical approval

This article is original and not published elsewhere. All authors discussed the results, read and approved the final manuscript. The authors confirm that there are no ethical issues in publication of the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Al-Hinai AH, Al-Sadi AM, Al-Bahry SN, Mothershaw AS, Al-Said AA, Al-Harthi SA, Deadman ML (2010) Isolation and characterization of Pseudomonas aeruginosa with antagonistic activity against Pythium aphanidermatum. J Plant Pathol 92:653–660Google Scholar
  2. Al-Hussini HS, Al-Rawahi AY, Al-Marhoon AA, Al-Abri SA, Al-Mahmooli IH, Al- Sadi AM, Velazhahan R (2019) Biological control of damping-off of tomato caused by Pythium aphanidermatum by using native antagonistic rhizobacteria isolated from Omani soil. J Plant Pathol 101:315–322CrossRefGoogle Scholar
  3. Al-Sadi AM, Al-Masoudi RS, Al-Habsi N, Al-Said FA, Al-Rawahy SA, Ahmed M, Deadman ML (2010) Effect of salinity on pythium damping-off of cucumber and on the tolerance of Pythium aphanidermatum. Plant Pathol 59:112–120CrossRefGoogle Scholar
  4. Ashengroph M, Nahvi I, Zarkesh-Esfahani H, Momenbeik F (2011) Pseudomonas resinovorans SPR1, a newly isolated strain with potential of transforming eugenol to vanillin and vanillic acid. New Biotechnol 6:656–664CrossRefGoogle Scholar
  5. Baki AAA, Anderson JD (1973) Vigour determination in soybean seed by multiple criteria. Crop Sci 31:630–633CrossRefGoogle Scholar
  6. Bakker PAHM, Lamers JG, Bakker AW, Marugg JD, Weisbeek PJ, Schippers B (1986) The role of siderophores in potato tuber yield increase by Pseudomonas putida in a short rotation of potato. Neth J Plant Pathol 92:249–256CrossRefGoogle Scholar
  7. Bouari AR, Begum SA, Maiga YEgiebor NO (2013) Biodegradation of cyanide complex compounds in aqueous media by Pseudomonas resinovorans. Environ Eng Sci 30:757–764CrossRefGoogle Scholar
  8. Ceccon E, Almazo-Rogel A, Martinez-Romero E, Toledo I (2012) The effect of inoculation of an indigenous bacteria on the early growth of Acacia farnesiana in a degraded area. CERNE 18:49–57CrossRefGoogle Scholar
  9. Chen J, Lin M, Huang J (2015) Efficacy of spent blewit mushroom compost and Bacillus aryabhattai combination on control of Pythium damping-off in cucumber. J Agr Sci 153:1257–1266CrossRefGoogle Scholar
  10. Cook RJ, Zhang BX (1985) Degrees of sensitivity to metalaxyl within the Pythium spp. pathogenic to wheat in the Pacific northwest. Plant Dis 69:686–688CrossRefGoogle Scholar
  11. Deadman M, Al-Hasani H, Al-Sadi A (2006) Solarization and biofumigation reduce Pythium aphanidermatum induced damping-off and enhance vegetative growth of greenhouse cucumber in Oman. J Plant Pathol 88:335–337Google Scholar
  12. Ferreira AM, Bonesso MF, Mondelli AL, Cunha MLRS (2012) Identification of Staphylococcus saprophyticus isolated from patients with urinary tract infection using a simple set of biochemical tests correlating with 16S–23S interspace region molecular weight patterns. J Microbiol Meth 91:406–411CrossRefGoogle Scholar
  13. Frank JA, Reich CI, Sharma S, Weisbaum JS, Wilson BA, Olsen GJ (2008) Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl Environ Microbiol 74:2461–2470CrossRefGoogle Scholar
  14. Goldstein J, Newbury DE, Joy DC, Lyman CE, Echlin P, Lifshin E, Sawyer L, Michael JR (2003) Scanning Electron microscopy and X-ray microanalysis, 3rd edn. Springer, Berlin, p 689CrossRefGoogle Scholar
  15. Halo BA, Al-Yahyai RA, Al-Sadi AM (2018) Aspergillus terreus inhibits growth and induces morphological abnormalities in Pythium aphanidermatum and suppresses Pythium-induced damping-off of cucumber. Front Microbiol 9:95CrossRefGoogle Scholar
  16. Han J, Sun L, Dong X, Cai Z, Sun X, Yang H, Wang Y, Song W (2005) Characterization of a novel plant growth-promoting bacteria strain Delftia tsuruhatensis HR4 both as a diazotroph and a potential biocontrol agent against various plant pathogens. Syst Appl Microbiol 28:66–76CrossRefGoogle Scholar
  17. Hickman GW, Michailides TJ (1998) Control options of greenhouse cucumber damping-off disease. J Veg Crop Prodn 4:45–48CrossRefGoogle Scholar
  18. Hubbard JP, Harman GE, Hadar Y (1983) Effect of soilborne Pseudomonas spp. on the biological control agent, Trichoderma hamatum, on pea seeds. Phytopathology 73:655–659CrossRefGoogle Scholar
  19. Kamala T, Indira S (2011) Evaluation of indigenous Trichoderma isolates from Manipur as biocontrol agent against Pythium aphanidermatum on common beans. 3 Biotech 1:217–225CrossRefGoogle Scholar
  20. Khabbaz SE, Abbasi PA (2014) Isolation, characterization and formulation of antagonistic bacteria for the management of seedlings damping-off and root rot disease of cucumber. Can J Microbiol 60:25–33CrossRefGoogle Scholar
  21. Moccellin R, dos Santos I, Heck DW, Malagi G, Dallemole-Giaretta R (2017) Control of cucumber damping-off caused by Pythium aphanidermatum using canola residues. Trop Plant Pathol 42:291–297CrossRefGoogle Scholar
  22. Nelson EB, Harman GE, Nash GT (1988) Enhancement of Trichoderma-induced biological control of Pythium seed rot and pre-emergence damping-off of peas. Soil Biol Biochem 20:145–150CrossRefGoogle Scholar
  23. Nicolopoulou-Stamati P, Maipas S, Kotampasi C, Stamatis P, Hens L (2016) Chemical pesticides and human health: the urgent need for a new concept in agriculture. Front Public Health 4:148CrossRefGoogle Scholar
  24. Postma J, Stevens LH, Wiegers GL, Davelaar E, Nijhuis EH (2009) Biological control of Pythium aphanidermatum in cucumber with a combined application of Lysobacter enzymogenesstrain 3.1T8 and chitosan. Biol Control 48:301–309CrossRefGoogle Scholar
  25. Preiswerk B, Ullrich S, Speich R, Bloemberg GV, Hombach M (2011) Human infection with Delftia tsuruhatensis isolated from a central venous catheter. J Med Microbiol 60:246–248CrossRefGoogle Scholar
  26. Punja ZK, Yip R (2003) Biological control of damping-off and root rot caused by Pythium aphanidermatum on greenhouse cucumbers. Can J Plant Pathol 25:411–417CrossRefGoogle Scholar
  27. Roberts DP, McKenna LF, Lakshman DK, Meyer SLF, Kong H, de Souza JT, Lydon J, Baker CJ, Buyer JS, Chung S (2007) Suppression of damping-off of cucumber caused by Pythium ultimum with live cells and extracts of Serratia marcescens N4-5. Soil Biol Biochem 39:2275–2288CrossRefGoogle Scholar
  28. Sanders PL (1984) Failure of metalaxyl to control Pythium blight on turfgrass in Pennsylvania. Plant Dis 68:776–777CrossRefGoogle Scholar
  29. Schreiter S, Sandmann M, Smalla K, Grosch R (2014) Soil type dependent rhizosphere competence and biocontrol of two bacterial inoculant strains and their effects on the rhizosphere microbial community of field-grown lettuce. PLoS One 9:e103726CrossRefGoogle Scholar
  30. Siddiqui IA, Shaukat SS (2003) Combination of Pseudomonas aeruginosa and Pochonia chlamydosporia for control of root-infecting fungi in tomato. J Phytopathol 151:215–222CrossRefGoogle Scholar
  31. Sivan A, Elad Y, Chet I (1984) Biological control effects of a new isolate of Trichoderma harzianum on Pythium aphanidermatum. Phytopathology 74:498–501CrossRefGoogle Scholar
  32. Xu X, Robinson J, Jeger M, Jeffries P (2010) Using combinations of biocontrol agents to control Botrytis cinerea on strawberry leaves under fluctuating temperatures. Biocontrol Sci Tech 20:359–373CrossRefGoogle Scholar
  33. Zegeye ED, Santhanam A, Gorfu D, Tessera M, Kassa B (2011) Biocontrol activity of Trichoderma viride and Pseudomonas fluorescens against Phytophthora infestans under greenhouse conditions. J Agr Technol 7:1589–1602Google Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Department of Crop Sciences, College of Agricultural and Marine SciencesSultan Qaboos UniversityMuscatSultanate of Oman

Personalised recommendations