Transcriptome and proteome analyses of proteases in biotroph fungal pathogen Cladosporium fulvum

  • Mansoor Karimi JashniEmail author
  • Ate van der Burgt
  • Evy Battaglia
  • Rahim Mehrabi
  • Jérôme Collemare
  • Pierre J. G. M. de Wit
Original Article


Proteases are key components of the hydrolytic enzyme arsenal employed by fungal pathogens to invade their host plants. The recent advances in -omics era have facilitated identification of functional proteases involved in plant-fungus interactions. By comparison of the publically available sequences of fungal genomes we found that the number of protease genes present in the genome of Cladosporium fulvum, a biotrophic tomato pathogen, is comparable with that of hemibiotrophs. To identify host plant inducible protease genes and their products, we performed transcriptome and proteome analyses of C. fulvumin vitro and in planta by means of RNA-Seq/RT-qPCR and mass spectrometry. Transcriptome data showed that 14 out of the 59 predicted proteases are expressed during in vitro and in planta growth of C. fulvum, of which nine belong to serine proteases S8 and S10 and the rest belong to metallo- and aspartic proteases. Mass spectrometry confirmed the presence of six proteases at proteome level during plant infection. Expression of limited number of proteases by C. fulvum might sustain biotrophic growth and benefits its stealth pathogenesis.


Fungal proteases Gene expression Tomato pathogen Cladosoprium fulvum Plant-microbe interaction 



We are grateful to financial assistance for this research provided by Wageningen University and the Ministry of Science, Research and Technology (MSRT) of Iran. We do thank Dr. S. Boren in department of Biochemistry-Wageningen University for performing mass spectrometry analysis.

Author contributions

MKJ, JC, RM and PJGMdW conceived the project. MKJ, JC and AVB performed the statistical and bioinformatics analyses. MKJ have performed the mass spectrometry analysis. MKJ and EB carried out the experimental work. MKJ wrote the manuscript. All authors have approved the manuscript and agree with submission to JPP.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

Supplementary material

42161_2019_433_MOESM1_ESM.docx (461 kb)
ESM 1 (DOCX 461 kb)


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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Laboratory of PhytopathologyWageningen UniversityWageningenThe Netherlands
  2. 2.Department of Plant PathologyTarbiat Modares UniversityTehranIran
  3. 3.DuPont Industrial Biosciences WageningenWageningenThe Netherlands
  4. 4.Fungal Natural ProductsWesterdijk Fungal Biodiversity InstituteUtrechtThe Netherlands
  5. 5.Department of Biotechnology, College of AgricultureIsfahan University of TechnologyIsfahanIran
  6. 6.Centre for BioSystems GenomicsWageningenThe Netherlands

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