Nocardiopsis dassonvillei strain MB22 from the Algerian Sahara promotes wheat seedlings growth and potentially controls the common root rot pathogen Bipolaris sorokiniana
- 2 Downloads
The biocontrol potential and plant-growth-promotion activities of seventeen actinobacteria, isolated from roots of native plants and soils harvested in the Algerian Sahara, were investigated. All strains were tested in vitro against five soil-borne phytopathogenic fungi. Six out of seventeen strains were tested in vivo for their biocontrol potential against Bipolaris sorokiniana strain LB12 as well as for their growth-promotion activities of durum wheat (cv. Vitron) seedlings via seed treatments in sterilized and non-sterilized soils. The root rot disease severity index (DSI) of wheat plants derived from seeds treated with actinobacteria (biological seed treatment) was significantly lower (p < 0.05) compared with those obtained from non-treated wheat seeds sown in inoculated pots (positive control). The strain Nocardiopsis dassonvillei MB22 showed the highest biocontrol activity with the DSI much lower than that of the positive control (27.7% versus 90.8% when non-treated seeds were sown in infested soil). No significant difference was observed between the DSI of plants obtained from biological seed treatments with the strain MB22 and those derived from seeds treated with the chemical fungicide Difeconazole®. Biological seed treatment with the spores of the strain MB22 resulted in greatest increases in the dry weight, the root and shoot length of seedlings. In in vitro tests, the strain MB22 was positive for the production of indole-3-acetic acid, siderophores, hydrogen cyanide, showed chitinolytic activity and solubilized inorganic phosphates which are commonly known features for the plant-growth-promotion and biocontrol activities of a given strain.
KeywordsDamping-off Durum wheat Nocardiopsis dassonvillei Seed germination Seedling emergence Sustainable agriculture
We thank Dr. Jay-Ram Lamichhane (French National Institute for Agricultural Research, Agrosystèmes et Agricultures, Gestion des Ressources, Innovations et Ruralités (AGIR)) for his kind contribution in the data processing and in revising the English of this article.
Compliance with ethical standards
Conflict of interest
Authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Aulakh KS, Kaur S, Chahal SS, Randhawa HS (1988) Seed borne Drechslera species in some important crops. Plant Dis Res 3:156–171Google Scholar
- Bhosale HJ, Kadam TA (2015) Generic diversity and a comparative account on plant growth promoting characteristics of actinomycetes in roots and rhizosphere of Saccharum officinarum. Int J Curr Microbiol App Sci 4:230–244Google Scholar
- Boukaya N, Goudjal Y, Zamoum M, Chaabane Chaouch F, Sabaou N, Mathieu F, Zitouni A (2018) Biocontrol and plant-growth-promoting capacities of actinobacterial strains from the Algerian Sahara and characterisation of Streptosporangium becharense SG1 as a promising biocontrol agent. Biocontrol Sci Tech 28:858–873CrossRefGoogle Scholar
- Dubin HJ, vanGinkel M (1991) The status of wheat diseases and disease research in warmer seas. In: Saunders DA (ed) Wheat for the Non-traditional Warm Areas. CIMMYT, Mexico DF, pp 125–135Google Scholar
- Duveiller E, Gilchrist LI (1994) Production constraints due to Bipolaris sorokiniana in wheat: current situation and future prospects. In: Saunders D, Hettel G (eds) Wheat in Heat-Stressed Environments: irrigated, dry Areas and rice-wheat farming systems (pp 343–352). Proceedings of the CIMMYT/UNDP workshop, Nashipur (Dinajpur), Bangladesh, February 1993Google Scholar
- Ellis MB (1971) Dematiaceous Hyphomycetes. Commonwealth Mycological, EnglandGoogle Scholar
- Flint ML (1990) Integrated Pest Management for Small Grains. University of California Division of agriculture and natural resources publicationGoogle Scholar
- Goudjal Y, Toumatia O, Sabaou N, Barakate M, Mathieu F, Zitouni A (2013) Endophytic actinomycetes from spontaneous plants of Algerian Sahara: indole-3-acetic acid production and tomato plants growth promoting activity. World J Microbiol Biotechnol 29:1821–1829. https://doi.org/10.1007/s11274-013-1344-y CrossRefGoogle Scholar
- Hadj Rabia-Boukhalfa Y, Eveno Y, Karama S, Selama O, Lauga B, Duran R, Hacène H, Eparvier V (2017) Isolation, purification and chemical characterization of a new angucyclinone compound produced by a new halotolerant Nocardiopsis sp. HR-4 strain. World J Microbiol Biotechnol 33:2292–2298. https://doi.org/10.1007/s11274-017-2292-8 CrossRefGoogle Scholar
- Husson F, Lê S, Pagès J (2009) Exploratory multivariate analysis by example using R. CRC Press/ Chapman and Hall/CRC Computer Science and Data Analysis, LondonGoogle Scholar
- Monteiro P, Borba MP, Van Der Sand ST (2017) Evaluation of the antifungal activity of Streptomyces sp. on Bipolaris sorokiniana and the growth promotion of wheat plants. J Agric Sci 9:229–240Google Scholar
- NIPPA (National Institute for Plant Protection in Algeria) (2016) Problematic of root rot and crown diseases of wheat: identification and distribution of species in the potentially cereal areas. Phyto Info Bull 51:6–8Google Scholar
- Velusamy P, Kim KY (2011) Chitinolytic activity of Enterobacter sp. KB3 antagonistic to Rhizoctonia solani and its role in the degradation of living fungal hyphae. Int Res J Microbiol 2:206–214Google Scholar
- Willy HV (2010) Soils, plant growth and crop production-Volume3. EOLSS PublisherGoogle Scholar