Development of pathogenicity assay and characterization of Fusarium oxysporum f. sp. elaeidis (FOE) based on Secreted In Xylem genes and EF-1α
The pathogenicity of four Fusarium oxysporum isolates collected from symptomatic oil palm (Elaeis guineensis Jacq.) from Ghana were tested for the first time to develop a new pathogenicity assay for Fusarium oxysporum f. sp. elaeidis (FOE) infection in oil palm seedlings. All four FOE isolates used for pathogenicity assay were pathogenic to oil palm seedlings within a relatively short time compared to other pathogenicity studies, for which infection/symptoms in oil palm seedlings was time consuming. FOE and “presumed-FOE” (i.e. Fusarium isolates collected from symptomatic oil palm trees whose pathogenicity is not confirmed) were characterised based on partial sequences of a housekeeping gene EF-1α and three Secreted In Xylem genes (SIX8, SIX9 and SIX11). All the phylogenetic trees generated for EF-1α, SIX8, SIX9 and SIX11 showed some variation between FOE, and “presumed-FOE”, but could not cluster isolates based on geographical location. Phylogenetic trees for EF-1α and SIX (SIX9 and SIX11) genes clustered both FOE and “presumed-FOE” from FUSARIUM-ID from GenBank, but SIX8 could not.
KeywordsPhylogenetics Presumed-FOE Symptomatic Effector proteins Elaeis guineensis Jacq.
This research was fully funded by Commonwealth Scholarship Commission-UK. We thank Dr. Julie Flood the Global Director of CABI Research for technical advice and support and the late Prof. Richard Cooper former Lecturer at University of Bath – UK for providing us with two isolates of FOE. Further thanks go to Dr. Y. Ndede a Scientist and Frank Dwumfour a Technician at the CSIR-OPRI, Ghana for the immense assistance during the field work. Our appreciation also goes to University of Nottingham – UK, Plant Science School of Biosciences for giving us access to laboratory facilities. We also appreciate the support of bioinformatician Dr. Preeti Panda of Scion-Forest Protection Team. We finally thank all the various oil palm plantation sites in Ghana where sampling was undertaken including CSIR-OPRI, NORPALM, BOPP and TOPP.
This study was funded by Commonwealth Scholarship Commission – UK with grant number (GHCS-115- 235 2013).
Compliance with ethical standards
Conflict of interest
Author Dr. Kwasi Adusei-Fosu currently a Forest Pathologist with Scion (New Zealand Forest Research Institute), received research grant from Commonwealth Scholarship Commission-UK and was supervised by Co-author Prof. Matthew Dickinson, Senior Plant Pathologist, Diagnostician Researcher, and Lecturer at the University of Nottingham – UK.
Also, there is no conflict of interest in existence: Author Dr. Kwasi Adusei-Fosu declares that he has no conflict of interest. Author Prof. Matthew Dickinson also declares that he has no conflict of interest.
This article does not contain any studies with human participants performed by any of the authors. Further, this article does not contain any studies with animals performed by any of the authors.
This article does not contain any studies with human participants or animals performed by authors.
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