First report of celery mosaic virus in Turkey
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KeywordsPotyvirus CeMV Detection
Celery mosaic virus (CeMV), which causes mosaic, vein-clearing, and dwarfing symptoms in celery (Apium graveolens), is a member of the genus Potyvirus in the family Potyviridae (Rose and Maiss 2018). In 2018–2019, symptoms reminiscent of CeMV were seen in celery plants in commercial celery production areas in the Çanakkale province, Turkey. Twenty-one symptomatic celery samples were collected for total nucleic acid extraction by the CTAB method (Li et al. 2008). Two-step RT-PCR was performed using a degenerate potyvirus-specific primer pair (Zheng et al. 2010). As a result, 19 out of 21 collected samples revealed a DNA band of about 350 bp in size, as expected. One of the RT-PCR products, designed as TRCNK, was randomly chosen, cloned into pGEM®-T easy vector system II (Promega, USA), and sequenced. The CeMV sequence was deposited in GenBank with the accession number MK575467. A BlastN analysis of the sequence against the GenBank database resulted in more than 96% identity with other CeMV isolates. To confirm the presence of CeMV, a new primer pair (CeMV_CPF_ 5’-GAGATGGCGGAGGAAGAA-3’ and CeMV_CPR_ 5’-ACTGCATAAACCGAAAGGA-3’) was designed to amplify the complete CeMV coat protein gene by RT-PCR. An amplicon (980 bp) of the expected size was obtained from the same 19 samples that tested positive in RT-PCR with the universal potyvirus primer pair. A randomly chosen RT-PCR product was purified and directly sequenced. The sequence of the complete CP gene (834 bp) was deposited in GenBank with the accession number MK570304. BlastN analysis of the sequence indicated 96–98% identity with other CeMV isolates for which sequence information is available in GenBank. To the best of our knowledge, this is the first report of CeMV in Turkey.
We would like to thank to Dr. Igor Koloniuk for constructive comments on this manuscript.
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Conflict of interest
All authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.