Advertisement

Journal of Plant Pathology

, Volume 101, Issue 4, pp 1159–1170 | Cite as

Evaluation of tomato genotypes for early blight disease resistance caused by Alternaria solani in Pakistan

  • Khalid Pervaiz AkhtarEmail author
  • Najeeb Ullah
  • Muhammad Yussouf Saleem
  • Qumer Iqbal
  • Muhammad Asghar
  • Abdul Rehman Khan
Short Communication
  • 38 Downloads

Abstract

Four hundred one tomato genotypes representing 11 species were screened against early blight (EB) disease caused by Alternaria solani in a net-house during 2013 to 2017. Considerable variation in resistance/susceptibility levels to EB was observed in the tested germplasm. Only one genotype ‘21396’ of Solanum lycopersicum was resistant and 56 were moderately resistant while the remaining were susceptible to highly susceptible. The pace of disease development was much slower in resistant genotype as symptoms were confined only to lower older leaves. The resistant genotype ‘21396’, most of the moderately resistant ones and a few susceptible and highly susceptible genotypes were rated resistant to stem and fruit infection. Resistant and moderately resistant S. lycopersicum genotypes and mutants with desirable characteristics could be grown in regions with high disease incidence, and can be used for the development of resistant/tolerant cultivars. The moderately resistant germplasm with very poor horticultural traits can nevertheless be used in resistance breeding programmes for developing superior cultivars and hybrids having new resistance genes to EB with a wider genetic base.

Keywords

Alternaria solani Early blight Genotypes rDNA-ITS Solanum spp. 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

42161_2019_304_MOESM1_ESM.txt (1 kb)
ESM 1 (TXT 556 bytes)

References

  1. Adhikari P, Oh Y, Panthee DR (2017) Current status of early blight resistance in tomato: an update. Int J Mol Sci 18:2019CrossRefGoogle Scholar
  2. Akhtar KP, Saleem MY, Iqbal Q, Asghar M, Hameed A, Sarwar N (2016) Evaluation of tomato genotypes for late blight resistance using low tunnel assay. J Plant Pathol 98:421–428Google Scholar
  3. Barksdale TH (1971) Field evaluation for tomato early blight resistance. Plant Dis Rep 55:807–809Google Scholar
  4. Barksdale TH, Stoner AK (1973) Segregation for horizontal resistance to tomato early blight. Plant Dis Rep 57:964–965Google Scholar
  5. Barksdale TH, Stoner AK (1977) A study of the inheritance of tomato early blight resistance. Plant Dis Rep 61:63–65Google Scholar
  6. Bessadat N, Berruyer R, Hamon B, Bataille-Simoneau N, Benichou S, Kihal M, Henni DE, Simoneau P (2017) Alternaria species associated with early blight epidemics on tomato and other Solanaceae crops in northwestern Algeria. Eur J Plant Pathol 148:181–197CrossRefGoogle Scholar
  7. Chaerani R, Voorrips RE (2006) Tomato early blight (Alternaria solani): the pathogen, genetics, and breeding for resistance. J Gen Plant Pathol 72:335–347CrossRefGoogle Scholar
  8. Chaerani R, Smulders MJM, Van Der Linden CG, Vosman B, Stam P, Voorrips RE (2007) QTL identification for early blight resistance (Alternaria solani) in a Solanum lycopersicum × S. arcanum cross. Theor Appl Genet 114:439–450CrossRefGoogle Scholar
  9. Datar VV, Mayee CD (1981) Assessment of losses in tomato yield due to early blight. Indian Phytopath 34:191–195Google Scholar
  10. Doyle JJ (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  11. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797CrossRefGoogle Scholar
  12. Foolad MR, Ashrafi H (2015) Characterization of early blight resistance in a recombinant inbred line population of tomato: I. heritability and trait correlations. Adv Stud Biol 7:131–148CrossRefGoogle Scholar
  13. Foolad MR, Ntahimpera N, Christ BJ, Lin GY (2000) Comparison between field, greenhouse, and detached-leaflet evaluations of tomato germplasm for early blight resistance. Plant Dis 84:967–972CrossRefGoogle Scholar
  14. Foolad M, Zhang L, Khan AA, Nino-Liu D, Lin G (2002) Identification of QTLs for early blight (Alternaria solani) resistance in tomato using backcross populations of a Lycopersicon esculentum × L. hirsutum cross. Theor Appl Genet 104:945–958CrossRefGoogle Scholar
  15. Gardner RG (2000) Plum Dandy’, a hybrid tomato, and its parents, NC EBR-5 and NC EBR-6. HortSci 35:962–963. (https://mountainhort.ces.ncsu.edu/wp-content/uploads/2017/03/release-NC-ERB5.pdf?fwd=no; https://mountainhort.ces.ncsu.edu/wp-content/uploads/2017/03/release-NC-ERB6.pdf?fwd=no). Accessed March 2017
  16. Holm AL, Rivera VV, Secor GA, Gudmestad NC (2003) Temporal sensitivity of Alternaria solani to foliar fungicides. Am J Potato Res 80:33–40CrossRefGoogle Scholar
  17. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  18. Pandey KK, Pandey PK, Kalloo G, Banerjee MK (2003) Resistance to early blight of tomato with respect to various parameters of disease epidemics. J Gen Plant Pathol 69:364–371CrossRefGoogle Scholar
  19. Pasche JS, Wharam CM, Gudmestad NC (2004) Shift in sensitivity of Alternaria solani in response to QoI fungicides. Plant Dis 88:181–187CrossRefGoogle Scholar
  20. Peralta IE, Knapp S, Spooner DM (2005) New species of wild tomatoes (Solanum section Lycopersicon: Solanaceae) from Northern Peru. Syst Bot 30:424–434Google Scholar
  21. Poysa V, Tu JC (1996) Response of cultivars and breeding lines of Lycopersicon spp. to Alternaria solani. Can Plant Dis Surv 76:5–8Google Scholar
  22. Saleem MY, Akhtar KP, Iqbal Q, Asghar M, Hameed A, Shoaib M (2016) Development of tomato hybrids with multiple disease tolerance. Pak J Bot 48(2):771–778Google Scholar
  23. Shah TM, Atta BM, Mirza JI, Haq MA (2009) Screening of chickpea (Cicer arietinum) induced mutants against Fusarium wilt. Pak J Bot 41:1945–1955Google Scholar
  24. Singh PC, Kumar R, Singh M, Rai A, Singh MC, Rai M (2011) Identification of resistant sources against early blight disease of tomato. Indian J Hort 68:516–521Google Scholar
  25. Spooner DM, Peralta IE, Knapp S (2005) Comparison of AFLPs with other markers for phylogenetic inference in wild tomatoes [Solanum L. section Lycopersicon (Mill.) Wettst.]. Taxon 54:43–61CrossRefGoogle Scholar
  26. Stancheva I, Lozanov I, Achkova Z (1991a) Sources of resistance to Alternaria solani in the tomato in wild growing species of the genus Lycopersicon. Genetika-i-Selektsiya 24:126–130Google Scholar
  27. Stancheva I, Lozanov I, Stamova L (1991b) Correlations between the resistance to different injuries from Alternaria solani in the tomatoes. Genetika-i-Selektsiya 24:51–55Google Scholar
  28. Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526PubMedPubMedCentralGoogle Scholar
  29. Thirthamallappa, Lohithaswa H (2000) Genetics of resistance to early blight (Alternaria solani Sorauer) in tomato (Lycopersicon esculentum L.). Euphytica 113:187–193CrossRefGoogle Scholar
  30. van der Waals JE, Korsten L, Slippers B (2004) Genetic diversity among Alternaria solani isolates from potatoes in South Africa. Plant Dis 88:959–964CrossRefGoogle Scholar
  31. Zhang LP, Lin GY, Nino-Liu D, Foolad MR (2003) Mapping QTLs conferring early blight (Alternaria solani) resistance in a Lycopersicon esculentum × L. hirsutum cross by selective genotyping. Mol Breed 12:3–19CrossRefGoogle Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Nuclear Institute for Agriculture and BiologyFaisalabadPakistan

Personalised recommendations