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Journal of Plant Pathology

, Volume 101, Issue 4, pp 907–916 | Cite as

Genetic and pathogenic variability of Rhizoctonia solani causing crown and root rot on sugar beet in France

  • Eliane Mayumi Inokuti
  • Delphine Thiery-Lanfranchi
  • Véronique Edel-HermannEmail author
  • Nadine Gautheron
  • Léon Fayolle
  • Sami Jorge Michereff
  • Christian Steinberg
Original Article

Abstract

Rhizoctonia crown and root rot causes severe damage on sugar beet. We collected twenty-seven isolates of Rhizoctonia solani from diseased sugar beets in France and Belgium over two years. All of them were pathogenic on sugar beet but displayed variable aggressiveness levels. Based on their internal transcribed spacer (ITS) sequences, we identified them as belonging to the anastomosis group (AG) 2–2. All isolates were further classified within the subgroup AG-2-2 IIIB based on their ability to grow at 35 °C. As we observed genetic diversity within and among isolates, we clarified heterogeneity within isolates by cloning their ITS region and sequencing 129 clones. The genetic variability levels among clones of a same isolate and among isolates were similar, suggesting that the 27 isolates were closely related. Reference isolates of R. solani AG-2-2 IIIB were genetically more diverse than the isolates collected in our study. The collection of R. solani established in this study provides resources for future investigations on the diversity and evolution of pathogenic populations and for breeding programs.

Keywords

AG-2-2, aggressiveness Anastomosis group Beta vulgaris Diversity Internal transcribed spacer 

Notes

Acknowledgments

The authors are grateful to the project partners who actively contributed to the collection of R. solani isolates characterized in this project: B. Richard and S. Houdault (GEVES); L. Boisroux (Delplanque); M. Butscha (Cristal Union); E. De Bruyne (SESVanderHave); E. Dubert and B. Lennefors (Syngenta); V. Dufayet and L. Falchetto (INRA); N. Henry (Florimond-Desprez); G. Malatesta (ITB); P. Mariotte and B. Holtschulte (KWS-SAAT); M. Stange and A. Schechert (Strube Research GmbH & Co. KG.). The authors would like to thank B. Hanse and J. Nechwatal for providing strains. This work was supported by the Compte d’Affectation Spéciale “Développement Agricole et Rural” (CASDAR) (R2B project n° 1C-2013-01). The first author was supported by a grant from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Not applicable

Supplementary material

42161_2019_289_MOESM1_ESM.pdf (273 kb)
ESM 1 (PDF 273 kb)
42161_2019_289_MOESM2_ESM.pdf (68 kb)
ESM 2 (PDF 68 kb)

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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Agroécologie, AgroSup Dijon, INRAUniv. Bourgogne Franche-ComtéDijonFrance
  2. 2.Departamento de AgronomiaUniversidade Federal Rural de PernambucoRecifeBrazil

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