Journal of Plant Pathology

, Volume 101, Issue 4, pp 1229–1229 | Cite as

First report of citrus leaf blotch virus in Satsuma mandarin in Korea

  • Chung Youl Park
  • Jeongran Park
  • Hongsup Kim
  • Seung-In Yi
  • Jae Sun MoonEmail author
Open Access
Disease Note


Citurs leaf blotch virus RNA sequencing Satsuma mandarin 

Satsuma mandarin (Citrus unshiu) is an economically important fruit in Korea, and more than 90% of the fruit is produced in Jeju Island. To determine the presence of viruses in satsuma mandarin, a survey was performed in Jeju Island in 2017. Leaf samples were collected from three trees with virus-like symptoms such as leaf blotch, chlorosis, distortion, and enation. All samples were mixed and ground in liquid nitrogen. Total RNA was subsequently extracted from this mixed sample by the NucliSENS EasyMAG biorobot (Biomérieux, Marcy l’Etoile, France) for RNA sequencing. A total of 295,758,098 reads obtained via Illumina HiSeq 100 bp paired-end RNA sequencing were analyzed as described by Lim et al. (2017). The resulting four viruses (citrus leaf blotch virus, CLBV; citrus tristeza virus, CTV; citrus vein enation virus, CVEV; and satsuma dwarf virus, SDV) were identified. Twenty-one contigs with high sequence similarities to citrus leaf blotch virus (CLBV), with an average length of 1319 bp, were identified. The largest contig (2112 bp) had 92% sequence identity with the CLBV NZ_G78 isolate (EU857540). A primer pair, CLB-F (5′-GCAAGACACTCTGTCTCAAAT-3′) and CLB-CP-R (5′-ATAGGTATCTACTCCAAAAAGG-3′), was designed according to the contig sequence and used to test all samples in reverse transcription (RT)-PCR. An amplicon of the expected size (214 bp) was obtained from three trees, and CLBV-infection was confirmed by direct sequencing. Of the three CLBV-positive leaf samples, two exhibited blotch symptoms, and one exhibited chlorosis. To determine the complete sequences of CLBV coat protein gene isolated from satsuma mandarin, RT-PCR using a primer set (5′-ACTGCCAGTGACACTGGGA-3′ and 5′-GTGTAAAGTCCTGGCCCAC-3′) was performed on one CLBV-positive sample, and an amplicon (1228 bp) was purified, cloned, and sequenced. Sequence analysis showed that this isolate (GenBank Accession No. LC416591) shared 97–99% nucleotide sequence identity with previously reported CLBV isolates from citrus plants. To the best of our knowledge, this is the first report of CLBV in Korea. CLBV could infect sweet cherry and kiwifruit (Chavan et al. 2013; Wang et al. 2016). Therefore, further investigation is needed to assess the extent and impact of CLBV infections in Korea.



This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Agri-Bio industry Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA)(317006).


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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Chung Youl Park
    • 1
    • 2
  • Jeongran Park
    • 1
  • Hongsup Kim
    • 1
  • Seung-In Yi
    • 1
  • Jae Sun Moon
    • 3
    Email author
  1. 1.Seed Testing & Research CenterKorea Seed & Variety ServiceGimcheonSouth Korea
  2. 2.Seed Vault DepartmentBaekdudaegan National ArboretumBonghwaSouth Korea
  3. 3.Plant Systems Engineering Research CenterKorea Research Institute of Bioscience and BiotechnologyDaejeonSouth Korea

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