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Journal of Plant Pathology

, Volume 101, Issue 4, pp 1127–1131 | Cite as

Partial characterization of a divergent isolate of pokeweed mosaic virus from Mississippi

  • Nina Aboughanem-Sabanadzovic
  • Thomas W. Allen
  • Alan Henn
  • Amanda Lawrence
  • Sead SabanadzovicEmail author
Short Communication
  • 14 Downloads

Abstract

An isolate of pokeweed mosaic virus (PkMV), referred to as MSFR03, collected from southwestern Mississippi was partially characterized biologically and molecularly. The virus induced pinwheel-like inclusions in infected cells of its natural host (Phytolacca americana L.) and was mechanically transmitted to Chenopodium quinoa, provoking localized infections. The viral genome is 9516 nt in size, excluding the 3′-terminal poly(A) tail, and contains a large ORF encoding a 3056 amino acids long polyprotein and a small overlapping PIPO ORF. Phylogenetic analyses of the whole polyprotein grouped PkMV with turnip mosaic virus (TuMV), potato virus A (PVA) and related potyviruses. Pairwise sequence comparisons showed that PkMV-MSFR03 is a distinct molecular variant of the virus, differing by more than 15% in nucleotide sequences with any of the four previously sequenced isolates from Pennsylvania, Maryland, New Jersey and Arkansas. Our data suggest that the PkMV population in the United States is genetically more diverse than previously known.

Keywords

Pokeweed Mosaic disease Virus Potyvirus Mississippi 

Notes

Acknowledgements

This publication is a contribution of the Mississippi Agricultural and Forestry Experiment Station. This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch project under accession number 226229.

Funding

This study was partially funded by Strategic Research Initiative of MAFES-MSU.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

42161_2019_277_MOESM1_ESM.docx (39 kb)
ESM 1 (DOCX 38 kb)

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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Institute for Genomics, Biocomputing and BiotechnologyMississippi State UniversityMississippi StateUSA
  2. 2.Delta Research and Extension CenterMississippi State UniversityStonevilleUSA
  3. 3.Department of Biochemistry, Molecular Biology, Entomology and Plant PathologyMississippi State UniversityMississippi StateUSA
  4. 4.Institute for Imaging and Analytical TechnologiesMississippi State UniversityMississippi StateUSA

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