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Survey of grapevine pathogens in Pakistan

  • Sunniya Rasool
  • Shagufta NazEmail author
  • Adib Rowhani
  • Alfredo Diaz-Lara
  • Deborah A. Golino
  • Kristen D. Farrar
  • Maher Al RwahnihEmail author
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Abstract

Grapevines from 13 vineyards in Pakistan were surveyed for the prevalence of several pathogens. Using RT-qPCR, 257 samples were tested for 19 viruses, phytoplasmas and Xylella fastidiosa. Prevalent viruses were: grapevine virus A (GVA, 47.8%), grapevine leafroll-associated virus 2 (GLRaV-2, 37.3%), grapevine rupestris stem pitting-associated virus (GRSPaV, 36.1%), and grapevine fleck virus (GFkV, 35%). Other viruses detected were: grapevine leafroll-associated virus 1 (GLRaV-1, 2.3%), grapevine leafroll-associated virus 2RG (GLRaV-2RG, 5%), grapevine leafroll-associated virus 3 (GLRaV-3, 7%), grapevine leafroll-associated virus 4 (GLRaV-4) and its strains (5, 6, and Pr, 16.6%), grapevine leafroll-associated virus 7 (GLRaV-7, 4.2%), grapevine fanleaf virus (GFLV, 11.6%), grapevine virus B (GVB, 4.2%), grapevine virus D (GVD, 0.7%), grapevine virus E (GVE, 1.1%), and grapevine Pinot gris virus (GPGV, 1.9%). Mixed infections were detected in 75.9% of samples. Pathogens tested for, but not detected include GLRaV-4 strains 9 and Car, grapevine red blotch virus (GRBV), tomato ringspot virus (ToRSV), tobacco ringspot virus (TRSV), Arabis mosaic virus (ArMV), grapevine virus F (GVF), phytoplasmas and X. fastidiosa. Additionally, 16 samples were analyzed by high-throughput sequencing (HTS) to confirm RT-qPCR results. In this paper we present an extensive survey for grapevine pathogens and thus the first report of GVA, GVB, GVD, GVE, GRSPaV, GFkV, GLRaV-1, GLRaV-2, GLRaV-2RG, GLRaV-3, GLRaV-4, GLRaV-4 strains 5, 6, and Pr, and GLRaV-7 in Pakistan.

Keywords

Virus detection RT-qPCR HTS Vitis vinifera 

Notes

Acknowledgements

This publication was made possible by support provided by the U.S. Agency for International Development through the Pakistan – U.S. Science & Technology Cooperation Program. The opinions expressed herein are those of the author(s) and do not necessarily reflect the views of the U.S. Agency for International Development. Additional funding was provided by the Higher Education Commission, Pakistan.

Supplementary material

42161_2019_263_MOESM1_ESM.docx (27 kb)
Supplementary Table 1 (DOCX 26 kb)

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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019
corrected publication 2019

Authors and Affiliations

  • Sunniya Rasool
    • 1
  • Shagufta Naz
    • 1
    Email author
  • Adib Rowhani
    • 2
  • Alfredo Diaz-Lara
    • 2
  • Deborah A. Golino
    • 2
  • Kristen D. Farrar
    • 3
  • Maher Al Rwahnih
    • 2
    Email author
  1. 1.Department of BiotechnologyLahore College for Women UniversityLahorePakistan
  2. 2.Department of Plant PathologyUniversity of California-DavisDavisUSA
  3. 3.Foundation Plant ServicesDavisUSA

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