Advertisement

Journal of Plant Pathology

, Volume 101, Issue 2, pp 361–366 | Cite as

Isolation and characterization of Xanthomonas euvesicatoria pv. euvesicatoria causing bacterial spot in Physalis pubescens in Northeast China

  • Shuang Song
  • Yao Zhang
  • Huan Liu
  • Chun-Qing Pan
  • Ming-Xiu Yang
  • Jing-Feng Ding
  • Jun-Hua ZhangEmail author
Short Communication
  • 48 Downloads

Abstract

Ground cherry (Physalis pubescens) is widely cultivated in Northeast China as one of its popular fruits. In the summer of 2017, suspected bacterial disease symptoms consisting of water-soaked spots and brown interveinal necrotic lesions with chlorotic margins were observed on ground cherry leaves in a field of Hailun County in Northeast China. From infected leaves, bacteria with pale-yellow, mucoid, domed circular colonies were repeatedly isolated. Based on biochemical tests and genetic characterizations of hrpB and concatenated four housekeeping genes (lepA-gyrB-gapA-gltA), the bacteria isolates obtained in this study were identified as Xanthomonas euvesicatoria pv. euvesicatoria. Pathogenicity of these isolates was confirmed on ground cherry, tomato and pepper seedlings by artificial inoculation. It was further confirmed that none of the related pathogens X. vesicatoria, X. gardneri or X. euvesicatoria pv. perforans were detected by species-specific PCR in field samples. To our knowledge, this is the first report of X. euvesicatoria pv. euvesicatoria causing bacterial spot of ground cherry in China.

Keywords

Ground cherry Bacteria hrpB Multilocus sequence analysis 

Notes

Acknowledgments

This work was supported by the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (No. uNPYSCT-2018157), the “Young Talents” Project of Northeast Agricultural University (No. 17QC05) and the Natural Science Foundation of Heilongjiang Province, China (No. C2017032).

Funding

This study was funded by the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (No. uNPYSCT-2018157), the “Young Talents” Project of Northeast Agricultural University (No. 17QC05) and the Natural Science Foundation of Heilongjiang Province, China (No. C2017032).

Compliance with ethical standards

Conflict of interest

All the authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

References

  1. Afsah AFE (2015) Survey of insects & mite associated cape gooseberry plants (Physalis peruviana L.) and impact of some selected safe materials against the main pests. Ann Agric Sci 60:183–191CrossRefGoogle Scholar
  2. Almeida NF, Yan S, Cai R, Clarke CR, Morris CE, Schaad NW, Schuenzel EL, Lacy GH, Sun X, Jones JB, Castillo JA, Bull CT, Leman S, Guttman DS, Setubal JC, Vinatzer BA (2010) PAMDB, a multilocus sequence typing and analysis database and website for plant-associated microbes. Phytopathology 100:208–215CrossRefGoogle Scholar
  3. Constantin EC, Cleenwerck I, Maes M, Baeyen S, Van Malderghem C, De Vos P, Cottyn B (2016) Genetic characterization of strains named as Xanthomonas axonopodis pv. dieffenbachiae leads to a taxonomic revision of the X. axonopodis species complex. Plant Pathol 65:792–806CrossRefGoogle Scholar
  4. Hao X, Zhang J, Li X, Wang W (2017) Application of a chitosan coating as a carrier for natamycin to maintain the storage quality of ground cherry (Physalis pubescens L.). J Zhejiang Univ Sci B 18:807–815CrossRefGoogle Scholar
  5. Ji L, Yuan Y, Ma Z, Chen Z, Gan L, Ma X, Huang D (2013) Induction of quinine reductase (QR) by withanolides isolated from Physalis pubescens L. (Solanaceae). Steroids 78:860–835CrossRefGoogle Scholar
  6. Jones JB, Lacy GH, Bouzar H, Stall RE, Schaad NW (2004) Reclassification of the Xanthomonads associated with bacterial spot disease of tomato and pepper. Syst Appl Microbiol 27:755–762CrossRefGoogle Scholar
  7. Kebede M, Timilsina S, Ayalew A, Admassu B, Potnis N, Minsavage GV, Goss EM, Hong JC, Strayer A, Paret M, Jones JB, Vallad GE (2014) Molecular characterization of Xanthomonas strains responsible for bacterial spot of tomato in Ethiopia. Eur J Plant Pathol 140:677–688CrossRefGoogle Scholar
  8. Koenraadt H, van Betteray B, Germain R, Hiddink G, Jones JB, Oosterhof J, Rijlaarsdam A, Roorda P, Woudt B (2009) Development of specific primers for the molecular detection of bacterial spot of pepper and tomato. Acta Hort 808:99–102Google Scholar
  9. Kyeon M, Son S, Noh Y, Kim Y, Lee H, Cha J (2016) Xanthomonas euvesicatoria causes bacterial spot disease on pepper plant in Korea. Plant Pathol J 32:431–440CrossRefGoogle Scholar
  10. Leyns F, Cleene MD, Swings J, Ley JD (1984) The host range of the genus Xanthomonas. Bot Rev 50:308–356CrossRefGoogle Scholar
  11. Obradovic A, Mavridis A, Rudolph K, Janse JD, Arsenijevic M, Jones JB, Minsavage GV, Wang J (2004) Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia. Eur J Plant Pathol 110:285–292CrossRefGoogle Scholar
  12. Osdaghi E, Taghavi SM, Hamzehzarghani H, Lamichhane JR (2016) Occurrence and characterization of the bacterial spot pathogen Xanthomonas euvesicatoria on pepper in Iran. J Phytopathol 164:722–734CrossRefGoogle Scholar
  13. Potnis N, Timilsina S, Strayer A, Shantharaj D, Barak JD, Paret ML, Vallad GE, Jones JB (2015) Bacterial spot of tomato and pepper: diverse Xanthomonas species with a wide variety of virulence factors posing a worldwide challenge. Mol Plant Pathol 16:907–920CrossRefGoogle Scholar
  14. Schaad NW, Jones JB, Chun W (2001) Laboratory guide for the identification of plant pathogenic bacteria, 3rd edn. American Phytopathological Society, St PaulGoogle Scholar
  15. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefGoogle Scholar
  16. Wonni I, Cottyn B, Detemmerman S, Dao S, Ouedraogo L, Sarra S, Tekete C, Poussier S, Corral R, Triplett L, Koita O, Koebnik R, Leach J, Szurek B, Maes M, Verdier V (2014) Analysis of Xanthomonas oryzae pv. oryzicola population in Mali and Burkina Faso reveals a high level of genetic and pathogenic diversity. Phytopathology 104:520–531CrossRefGoogle Scholar
  17. Young JM, Park DC, Shearman HM, Fargier E (2008) A multilocus sequence analysis of the genus Xanthomonas. Syst Appl Microbiol 31:366–377CrossRefGoogle Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018

Authors and Affiliations

  1. 1.Department of Plant Protection, College of AgricultureNortheast Agricultural UniversityHarbinChina
  2. 2.State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant ProtectionNorthwest A&F UniversityYanglingChina

Personalised recommendations