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Genetic analysis and molecular mapping of stripe rust resistance in an excellent wheat line Sanshumai1

  • Cai Sun
  • Peng Zhang
  • Zhengwu Fang
  • Xing Zhang
  • Junliang Yin
  • Dongfang Ma
  • Yongxing Zhu
Original Article

Abstract

Stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici (Pst), is one of the most widespread diseases in wheat (Triticum aestivum L.). Planting resistant cultivars has been constantly practiced for decades as the best strategy to defense against the variations of prevalent Pst races. The wheat line Sanshumai1 was developed from an interspecific hybridization of Haynaldia villosa (L) Schur. (2n = 2× = 14, VV)/Triticum turgidum L. var. durum (4n = 4× = 28, AABB)///Yanxiaomai. Sanshumai1 has all-stage resistance to most of known stripe rust races in China, including three widely virulent races CYR31, CYR32, and CYR33. To identify stripe rust resistance gene in this line, Sanshumai1 was crossed with the susceptible genotype, Mingxian169, and the F1, F2, F3, and BC1 generations were inoculated with Pst races under the controlled greenhouse conditions. The genetic results indicated that two stripe rust resistance genes in Sanshumai1, temporarily designated as YrS1 and YrS2, confer resistance to CYR31 and Su11–11, respectively. Using bulked segregant analysis (BSA) methodology, we identified five simple sequence repeat (SSR) markers and two expressed sequence tag-sequence tagged site (EST-STS) markers associated with YrS1 on the short arm of chromosome 3D. The genetic distances of the two closest flanking markers, namely Xcfd79 and Xwmc674, were 4.1 and 8.7 centiMorgans, respectively. In addition, we identified four SSR markers associated with YrS2 on the long arm of chromosome 4D. The genetic distances of the two closest flanking markers, namely Xcfd84 and Xgwm194, were 6.8 and 7.1 centiMorgans, respectively. Based on the chromosomal location, reaction patterns, and pedigree analysis, these two genes are likely novel resistance genes. These two genes and the flanking markers developed from this study are expected to be useful in pyramiding YrS1 and YrS2 with other Yr genes to develop wheat cultivars with high-level and durable resistance to stripe rust and may also benefit marker assisted selection (MAS) in breeding programs.

Keywords

Puccinia striiformis Sanshumai 1 Stripe rust Molecular mapping Resistance gene 

Notes

Acknowledgements

This research work was funded by the Natural Science Funds of Hubei Province of China (2016CFB478) and National Natural Science Foundation of China (No. 31501620) and the open project program of State Key Laboratory for Biology of Plant Diseases and Insect Pests (NO. SKLOF201707). Finally, we thank Prof. Qiao Yongli for beneficial comments on the initial project design and data analysis and Prof. Qingqin Zhang for providing research materials.

References

  1. Boukhatem N, Baret PV, Mingeot D, Jacquemin JM (2002) Quantitative trait loci for resistance against yellow rust in two wheat-derived recombinant inbred line populations. Theor Appl Genet 104:111–118.  https://doi.org/10.1007/s001220200013 CrossRefPubMedGoogle Scholar
  2. Chen XM (2005) Epidemiology and control of stripe rust [Puccinia striiformis f. sp. tritici] on wheat. Can J Plant Pathol 27:314–337.  https://doi.org/10.1080/07060660509507230 CrossRefGoogle Scholar
  3. Chen XM, Jones SS, Line RF (1995) Chromosomal location of genes for stripe rust resistance in spring wheat cultivars Compair, Fielder, Lee and Lemhi and interactions of aneuploid wheats with races of Puccinia striiformis. Phytopathology 85:375–381.  https://doi.org/10.1094/Phyto-85-375 CrossRefGoogle Scholar
  4. Chen XM, Line RF, Leung HL (1998) Genome scanning for resistance-gene analogs in rice, barley, and wheat by high-resolution electrophoresis. Theor Appl Genet 97:345–355.  https://doi.org/10.1007/s001220050905 CrossRefGoogle Scholar
  5. Chen WQ, Wu LR, Liu TG, Xu SC, Jin SL, Peng YL, Wang BT (2009) Race dynamics, diversity, and virulence evolution in Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust in China from 2003 to 2007. Plant Dis 93:1093–1101.  https://doi.org/10.1094/PDIS-93-11-1093 CrossRefGoogle Scholar
  6. Chen XM, Penman L, Wan AM, Cheng P (2010) Virulence races of Puccinia striiformis f. sp. tritici in 2006 and 2007 and development of wheat stripe rust and distributions, dynamics, and evolutionary relationships of races from 2000 to 2007 in the United States. Can J Plant Pathol 32:315–333.  https://doi.org/10.1080/07060661.2010.499271 CrossRefGoogle Scholar
  7. Chen WQ, Kang ZS, Ma ZH, Xu SC, Jin SL, Jiang YL (2013) Integrated management of wheat stripe rust caused by Puccinia striiformis f. sp. tritici in China. Sci Agric Sin 46:4254–4262.  https://doi.org/10.3864/j.issn.0578-1752.2013.20.008 CrossRefGoogle Scholar
  8. Dedryver F, Paillard S, Mallard S, Robert O, Trottet M, Nègre S, Verplancke G, Jahier J (2009) Characterization of genetic components involved in durable resistance to stripe rust in the bread wheat 'Renan'. Phytopathology 99:968–973.  https://doi.org/10.1094/PHYTO-99-8-0968 CrossRefPubMedGoogle Scholar
  9. Herrera-Foessel S, Lagudah ES, Huerta-Espino J, Hayden MJ, Bariana HS, Singh D, Singh RP (2011) New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked. Theor Appl Genet 122:239–249.  https://doi.org/10.1007/s00122-010-1439-x CrossRefPubMedGoogle Scholar
  10. Hovmøller MS, Walter S, Bayles RA, Hubbard A, Flath K, Sommerfeldt N, Leconte M, Czembor P, Rodriguez-Algaba J, Thach T, Hansen JG, Lassen P, Justesen AF, Ali S, Vallavieille-Pope C (2016) Replacement of the european wheat yellow rust population by new races from the Centre of diversity in the near-himalayan region. Plant Pathol 65:402–411.  https://doi.org/10.1111/ppa.12433 CrossRefGoogle Scholar
  11. Juliana P, Singh RP, Singh PK, Crossa J, Huerta-Espino J, Lan C, Bhavani S, Rutkoski JE, Poland JA, Bergstrom GC, Sorrells ME (2017) Genomic and pedigree-based prediction for leaf, stem, and stripe rust resistance in wheat. Theor Appl Genet theoretische Und Angewandte Genetik 130:1–16.  https://doi.org/10.1007/s00122-017-2897-1 CrossRefGoogle Scholar
  12. Kosambi DD (1943) The estimation of map distances from recombination values. Ann Hum Genet 12:172–175.  https://doi.org/10.1111/j.1469-1809 CrossRefGoogle Scholar
  13. Li Q, Xia T, Li JJ, Li GG, Wang F, Wang BT (2011) Determination of pathogenicrange of T4 new strains of Puccinia strifformis f.sp. tritici to ‘Zhou4’. Acta Phytopathol Sinica 41:604–610Google Scholar
  14. Lincoln Se, Daly Mj, Lander Es, Lincoln S, Daly M, Lander E, Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with Mapmarker/EXP3.0. Whitehead Institute Technical Report. Whitehead Institute. p. 3Google Scholar
  15. McIntosh R, Dubcovsky J, Rogers W (2013) Catalogue of gene symbols for wheat: 2013–2014 supplement [ER/OL]. http://www.shigen.nig.ac.jp/wheat/Komugi/genes/macgene/supplement2013.pdf
  16. McIntosh RA, Dubcovsky J, Rogers WJ, Morris CF, Appels R, Xia XC (2016) Catalogue of gene symbols for wheat: 2015–2016 supplement. https://shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2015.pdf
  17. McIntosh RA, Dubcovsky J, Rogers WJ, Morris CF, Appels R, Xia XC (2017) Catalogue of gene symbols for wheat: 2017 Supplement. https://shigen.nig.ac.jp/wheat/komugi/genes/macgene/ supplement2017.pdf
  18. Nelson J, Van Deynze AE, Autrique E, Sorrells ME, Lu YH, Negre S, Bernard M, Leroy P (1995) Molecular mapping of wheat. Homoeologous group 3. Genome 38:525–533.  https://doi.org/10.1046/j.1439-0523.2002.728336.x CrossRefPubMedGoogle Scholar
  19. Ren Y (2012) Molecular mapping of stripe rust resistance genes in common wheat. Chinese Acadamy of Agricultureal Science, BeijingGoogle Scholar
  20. Rogers SQ, Bendich AJ (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5:69–76.  https://doi.org/10.1007/BF00020088 CrossRefPubMedGoogle Scholar
  21. Singh RP, Nelson JC, Sorrells ME (2000) Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Sci 40:1148–1155.  https://doi.org/10.2135/cropsci2000.4041148x CrossRefGoogle Scholar
  22. Suenaga K, Singh RP, Huerta-Espino J, William HM (2003) Microsatellite markers for genes lr34/yr18 and other quantitative trait Loci for leaf rust and stripe rust resistance in bread wheat. Phytopathol 93:881–890.  https://doi.org/10.1094/PHYTO.2003.93.7.881 CrossRefGoogle Scholar
  23. Zheng SG, Li YF, Lu L, Liu ZH, Zhang CH, Ao DH, Li LR, Zhang CY, Liu R, Luo CP, Wu Y, Zhang L (2017) Evaluating the contribution of Yr genes to stripe rust resistance breeding through marker-assisted detection in wheat. Euphytica 213:50.  https://doi.org/10.1007/s10681-016-1828-6 CrossRefGoogle Scholar
  24. Zhou XL, Wang WL, Wang LL, Hou DY, Jing JX, Wang Y, Xu ZQ, Yao Q, Yin JL, Ma DF (2011) Genetics and molecular mapping of genes for high-temperature resistance to stripe rust in wheat cultivar Xiaoyan 54. Theoret Appl Genet 123:431–438.  https://doi.org/10.1007/s00122-011-1595-7 CrossRefGoogle Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018

Authors and Affiliations

  1. 1.Hubei Collaborative Innovation Center for Grain Industry/College of AgricultureYangtze UniversityJingzhouChina
  2. 2.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina
  3. 3.College of Horticulture and GardeningYangtze UniversityJingzhouChina

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