Journal of Plant Pathology

, Volume 101, Issue 1, pp 155–160 | Cite as

Standard area diagrams to aid assessments of the severity of blight leaf spot symptoms on cassava leaves

  • Iris Carolina Henrique Lima Leite
  • Francisco Alberto Sousa Lima Filho
  • Rejane Livramento Freitas-Lopes
  • Sami Jorge Michereff
  • Alexandre Sandri Capucho
  • Ueder Pedro LopesEmail author
Short Communication


A standard area diagram (SAD) set to assess the severity of blight leaf spot (BliLS) caused by Passalora vicosae on cassava leaves was developed and validated. The proposed SAD set includes diagrams illustrating six leaves with different severities of blight leaf spot (5, 10, 20, 30, 40 and 50%). The SAD set was validated by ten raters with no previous experience in disease assessment. Lin’s concordance correlation analysis of estimated versus actual disease severity showed that the statistical parameters (υ, u, r and ρc) were improved when raters were aided by the SADs. Overall agreement measured by Lin’s concordance correlation coefficient (ρc) increased from 0.70 to 0.82, without and with SADs, respectively. Similarly, by analysing the coefficient of determination (R2) and intra-class correlation coefficient (ρ), the estimates of severity were more reliable by using the SADs. The proposed SAD set increases the accuracy and reliability of estimates of the severity of BliLS in cassava and therefore is a valuable aid for research on the epidemiology, breeding for resistance, fungicide screening and pathotype characterization.


Manihot esculenta Passalora vicosae Diagrammatic scale Disease assessment Phytopatometry 



This work was supported by Fundação de Amparo à Ciência e Tecnologia de Pernambuco (APQ-1542-5.01/15) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (Universal CNPq 454010/2014-1).

Compliance with ethical standards

Conflict of interest

There is no conflict of interest in this work.

All forms of financial support are acknowledged in the contribution.

This work does not involve any human participants or animals.

All authors have offered their consent for the submission.


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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018

Authors and Affiliations

  • Iris Carolina Henrique Lima Leite
    • 1
  • Francisco Alberto Sousa Lima Filho
    • 1
  • Rejane Livramento Freitas-Lopes
    • 2
  • Sami Jorge Michereff
    • 2
  • Alexandre Sandri Capucho
    • 3
  • Ueder Pedro Lopes
    • 1
    Email author
  1. 1.Unidade Acadêmica de GaranhunsUniversidade Federal Rural de PernambucoGaranhunsBrazil
  2. 2.Departamento de AgronomiaUniversidade Federal Rural de PernambucoRecifeBrazil
  3. 3.Universidade Federal do Vale do São FranciscoPetrolinaBrazil

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