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Journal of Plant Pathology

, Volume 101, Issue 1, pp 1–14 | Cite as

Preliminary characterization of potato virus Y (PVY) populations in Algerian potato fields

  • Linda Allala-MessaoudiEmail author
  • Laurent Glais
  • Mohamed Kerkoud
  • Sonia Boukhris-Bouhachem
  • Zouaoui Bouznad
Original Article

Abstract

To date, only limited data are available concerning the viral pressure present in potato crops in Algeria. For three consecutive years, surveys were conducted in potato fields in the main Algerian potato-growing regions during the mid-season crop cycle (January to March). A total of 285 potato samples were characterized to define the prevalence of the five most common virus species: Potato virus Y (PVY), Potato leaf roll virus (PLRV), Potato virus X (PVX), Potato virus A (PVA) and Potato virus S (PVS). The results showed a higher incidence of PVY compared to the other four viruses. Because of this predominance of PVY and its distribution in all of the scouted regions, an analysis was carried out on the diversity of PVY populations. From a panel of 185 samples, serologically confirmed as being solely infected by PVY, 96.7% were found to be of serotype-N, and only 3.2% of serotype-O. A set of 31 PVY isolates was further analyzed by biotyping on tobacco and by molecular typing (RT-PCR, sequencing), targeting the nucleotide sequence polymorphism in the 5’NTR/P1 region and the three recombination junctions within the HC-Pro/P3 (RJ2), VPg/NIa (RJ3) and CP (RJ4) regions. All 28 PVY isolates from serotype-N inducing vein necrosis on tobacco were recombinant PVYNTN isolates. Among the three PVY isolates of serotype-O, two were typed as PVYN-Wi and induced vein necrosis on tobacco. This is the first report of the identification of NTN and Wilga type isolates in Algeria.

Keywords

PVYNTN PVYN-Wi Solanaceae Diversity Recombinant isolates 

Notes

Acknowledgements

The work presented has been supported by the Ecole Nationale Supérieure Agronomique (ENSA) of Algiers and the Centre National de Certification des plants et semences (CNCC) of Algiers and Adgen (Angers). We would like to thank Dr. Laala Samia for the excellent technical assistance provided with RT-PCR assays. We are grateful to Dr. Fekih Ibtissem, Dr. Didier Andrivon, and to Ms. Jackie Vasselon-Molloy for their careful revision of the written English.

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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018

Authors and Affiliations

  • Linda Allala-Messaoudi
    • 1
    Email author
  • Laurent Glais
    • 2
    • 3
  • Mohamed Kerkoud
    • 4
  • Sonia Boukhris-Bouhachem
    • 5
  • Zouaoui Bouznad
    • 1
  1. 1.Département de Botanique, Laboratoire de Virologie VégétaleÉcole Nationale Supérieure AgronomiqueEl-HarrachAlgeria
  2. 2.FN3PT/RD3PTParisFrance
  3. 3.IGEPP, Agrocampus Ouest, INRAUniversité de Rennes 1Le RheuFrance
  4. 4.Diag-genAngersFrance
  5. 5.INRAT, Laboratoire de Protection des VégétauxTunisTunisia

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