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Molecular detection and identification of phytoplasmas in a novel 16SrI subgroup in sunflowers and cocklebur weeds

  • Lei Zhang
  • Ping-ping Sun
  • Qiang MaEmail author
  • Xiao-zhao Xu
  • Hong-you Zhou
  • Zheng-nan LiEmail author
Short Communication
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Abstract

Sunflowers (Helianthus annuus L.) exhibiting symptoms of stem fasciation and cluster of multiple inflorescences were observed in Baicheng, Jilin province, China. Coincidently, cockleburs (Xanthium strumarium L.), a weed in the sunflower fields, showing similar symptoms were observed as well. Based on successful amplification of 16S rRNA and secY genes of phytoplasmas, the sunflower fasciation disease (SFD) and cocklebur fasciation disease (CFD) were determined to be associated with phytoplasmas. Based on virtual and actual RFLP analysis of the F2nR2 region of the 16S rRNA genes, the SFD and CFD associated phytoplasmas were classified into a novel 16SrI subgroup, designated 16SrI-AI. This was the first report of SFD associated phytoplasma in China, and the first evidence of phytoplasma infecting cocklebur worldwide.

Keywords

Sunflower fasciation Cocklebur fasciation Phytoplasma RFLP A novel subgroup of 16SrI-AI 

Notes

Acknowledgements

This research was funded by the State Key Laboratory of Crop Stress Biology for Arid Areas Open Project (CSBAA2016005). The authors are grateful to Dr. George Ochieng Asudi for giving great comments and suggestions on improving the manuscript, who is from Biochemistry and Biotechnology Department, Kenyatta University, Nairobi, Kenya and Bioinformatik und Molekulare Botanik, Friedrich-Schiller-University, Jena, Germany.

Funding

This study was funded by the State Key Laboratory of Crop Stress Biology for Arid Areas Open Project (CSBAA2016005).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

References

  1. Bertaccini A (2007) Phytoplasmas: diversity, taxonomy, and epidemiology. Front Biosci 12:673–689CrossRefGoogle Scholar
  2. Contaldo N, Bertaccini A, Paltrinieri S, Windsor HM, Windsor GD (2012) Axenic culture of plant pathogenic phytoplasmas. Phytopathol Mediterr 51:607–617Google Scholar
  3. Deng S, Hiruki C (1991) Amplification of 16S rRNA genes from culturable and nonculturable Mollicutes. J Microbiol Methods 14:53–61CrossRefGoogle Scholar
  4. Doi Y, Teranaka M, Yora K, Asuyama H (1967) Mycoplasma-or PLT group-like microorganisms found in the phloem elements of plants infected with mulberry dwarf, potato witches' broom, aster yellows, or paulownia witches' broom. Jpn J Phytopathol 33:259–266CrossRefGoogle Scholar
  5. Economou A (1999) Following the leader: bacterial protein export through the Sec pathway. Trends Microbiol 7:315–320CrossRefGoogle Scholar
  6. Fabre A, Danet J-L, Foissac X (2011) The stolbur phytoplasma antigenic membrane protein gene stamp is submitted to diversifying positive selection. Gene 472:37–41CrossRefGoogle Scholar
  7. Firrao G, Garcia-Chapa M, Marzachì C (2007) Phytoplasmas: genetics, diagnosis and relationships with the plant and insect host. Front Biosci 12:1353–1375CrossRefGoogle Scholar
  8. Gulya T, Mathew F, Harveson R, Markell S, Block C (2016) Diseases of sunflower. In: McGovern RJ, Elmer WH (eds) Handbook of Florists' crops diseases, handbook of plant disease management. Springer International Publishing, Cham, pp 1–20Google Scholar
  9. Gundersen D, Lee I-M (1996) Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathol Mediterr 35:144–151Google Scholar
  10. Gundersen D, Lee I-M, Schaff D, Harrison N, Chang C, Davis R, Kingsbury D (1996) Genomic diversity and differentiation among phytoplasma strains in 16S rRNA groups I (aster yellows and related phytoplasmas) and III (X-disease and related phytoplasmas). Int J Syst Evol Microbiol 46:64–75Google Scholar
  11. Harrison N, Helmick E (2008) First report of a ‘Candidatus Phytoplasma asteris’-related strain associated with little leaf disease of Helianthus debilis in Florida, USA. Plant Pathol 57:772–772CrossRefGoogle Scholar
  12. IRPCM, 2004. Spiroplasma Working Team-phytoplasma taxonomy group (2004) ‘Candidatus Phytoplasma’, a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects. Int J Syst Evol Microbiol 54:1243–1255CrossRefGoogle Scholar
  13. Kollar A, Seemüller E, Bonnet F, Saillard C, Bove J (1990) Isolation of the DNA of various plant pathogenic mycoplasmalike organisms from infected plants. Phytopathology 80:233–237CrossRefGoogle Scholar
  14. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  15. Lee I-M, Gundersen-Rindal DE, Davis RE, Bartoszyk IM (1998) Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int J Syst Bacteriol 48:1153–1169CrossRefGoogle Scholar
  16. Lee I-M, Zhao Y, Bottner K (2006) SecY gene sequence analysis for finer differentiation of diversestrains in the aster yellows phytoplasma group. Mol Cell Probes 20:87–91CrossRefGoogle Scholar
  17. Lee I-M, Bottner-Parker K, Zhao Y, Davis R, Harrison N (2010) Phylogenetic analysis and delineation of phytoplasmas based on secY gene sequences. Int J Syst Evol Microbiol 60:2887–2897CrossRefGoogle Scholar
  18. Maejima K, Oshima K, Namba S (2014) Exploring the phytoplasmas, plant pathogenic bacteria. J Gen Plant Pathol 80:210–221CrossRefGoogle Scholar
  19. Martini M, Lee I-M, Bottner K, Zhao Y, Botti S, Bertaccini A, Harrison N, Carraro L, Marcone C, Khan A (2007) Ribosomal protein gene-based phylogeny for finer differentiation and classification of phytoplasmas. Int J Syst Evol Microbiol 57:2037–2051CrossRefGoogle Scholar
  20. Mielke T (2018) World markets for vegetable oils and animal fats, dynamics of global production, trade flows, consumption and prices. In: Kaltschmitt M, Neuling U (eds) Biokerosene. Springer, Berlin, pp 147–188CrossRefGoogle Scholar
  21. Nejat N, Sijam K, Abdullah SNA, Vadamalai G, Dickinson M (2010) Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia. Afr J Biotechnol 9:2305–2315Google Scholar
  22. Schneider B, Gibb KS (1997) Sequence and RFLP analysis of the elongation factor Tu gene used in differentiation and classification of phytoplasmas. Microbiology 143:3381–3389CrossRefGoogle Scholar
  23. Schneider B, Seemüller E, Smart C, Kirkpatrick B (1995) Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. In: Razin S, Tully J (eds) Molecular and diagnostic procedures in Mycoplasmology. Academic Press, New York, pp 369–380CrossRefGoogle Scholar
  24. Seemüller E, Marcone C, Lauer U, Ragozzino A, Göschl M (1998) Current status of molecular classification of the phytoplasmas. J Plant Pathol 80:3–26Google Scholar
  25. Tazehkand SA, Pour AH, Heydarnejad J, Varsani A, Massumi H (2010) Identification of phytoplasmas associated with cultivated and ornamental plants in Kerman province, Iran. J Phytopathol 158:713–720CrossRefGoogle Scholar
  26. Tseng YW, Deng WL, Chang CJ, Shih HT, Su CC, Jan FJ (2016) The phytoplasma associated with purple woodnettle witches'-broom disease in Taiwan represents a new subgroup of the aster yellows phytoplasma group. Ann Appl Biol 169:298–310CrossRefGoogle Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.College of Horticulture and Plant ProtectionInner Mongolia Agricultural UniversityHuhhotChina
  2. 2.Department of Plant PathologyUniversity of FloridaGainesvilleUSA
  3. 3.Department of HorticultureQingdao Agricultural UniversityQingdaoChina

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