In vitro studies of α-TCP and β-TCP produced from Clinocardium ciliatum seashells

  • Yesim Muge SahinEmail author
  • Zeynep Orman
  • Sevil Yucel


In this study, α-TCP and β-TCP were successfully produced via a mechanochemical method from aragonite structures, Clinocardium ciliatum seashells, at 850 °C, 1000 °C, and 1200 °C sintering temperatures. The characterization of the obtained materials was carried out via FT-IR, SEM/EDX, BET, XRD, ICP-OES analysis. Samples were soaked for 21 days in simulated body fluid (SBF) for a bioactivity test. Additionally, MTT assay was applied to determine the cell viability of samples. Bioactivity in vitro tests showed that bone-like hydroxyapatite formed when the α-TCP and β-TCP were soaked in SBF. TCP bioceramics had a noncytotoxicity effect on SAOS-2 osteoblast-like cells and cell viability increased in 1 to 7 days. The produced bioceramics have ideal pore sizes and properties that are suitable for supporting biological activities. Tris-HCl buffer solution was used to obtain the level of biodegradation. It was seen that α-TCP exhibited better dissolution features than β-TCP.


Bioactivity Bone regeneration SBF Seashell TCP Tris 



Special thanks to ArelPOTKAM (Polymer Technologies and Composite Application and Research Center) where the synthesis of bioceramics took place.


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Copyright information

© Australian Ceramic Society 2019

Authors and Affiliations

  1. 1.ArelPOTKAM (Polymer Technologies and Composite Application and Research Center)Istanbul Arel UniversityIstanbulTurkey
  2. 2.Department of Biomedical EngineeringIstanbul Arel UniversityIstanbulTurkey
  3. 3.Department of BioengineeringYildiz Technical UniversityIstanbulTurkey

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