Enhanced in vivo biocompatibility of magnesia-contained bioactive glasses
Human blood compatibility of previously prepared magnesia-doped bioactive glass samples was evaluated by hemolysis assay. The in vivo evaluation was carried out using a rat model in which a hole was made in the rat femur bone by drilling and the bioactive glass samples were implanted in the cavity. Histological examination had demonstrated the better bone regeneration and blood vessel formation as compared to the reference one. The in vivo complete blood count (CBC) was also analyzed at different time periods. The in vivo results of magnesium-containing bioactive glasses had shown improved bioactivity as well as better biocompatibility.
KeywordsIn vivo MgO bioactive glasses Rat model Blood compatibility Histology
The authors gratefully acknowledge the Head, Department of Ceramic Engineering, IIT (BHU) and the honorable Director, IIT (BHU) Varanasi, India, for providing necessary facilities for the present research work. They are also very much thankful to the nodal officer of the GLP lab, National Facility for Tribal and Herbal Medicine (NFTHM), for conducting in vivo complete blood analysis.
The author Dr. Sampath Kumar Arepalli is also very much grateful to the UGC, New Delhi, India (F.14-2(SC)/2010(SA-III)), for providing Rajiv Gandhi National Fellowship for this work.
Compliance with ethical standards
Approval of Institutional Animal Ethics Committee (protocol no. Dean/2016/CAEC/45) was accorded before the beginning of the experiment. All the experiments were performed as per the guidelines of laboratory animal care (National Research Council US Committee for the Update of the Guide for the Care and Use of Laboratory Animals 2011) .
- 1.Walker, G.M.: Magnesium and cell cycle control: an update. Magnesium. 5(1), 9–23 (1986)Google Scholar
- 8.Arepalli, S.K., Tripathi, H., Manna, P.P., Pankaj, P., Krishnamurthy, S., Patne, S.C.U., Pyare, R., Singh, S.P.: Preparation and in vitro investigation on bioactivity of magnesia-contained bioactive glasses. J. Aust. Ceram. Soc. (2018). https://doi.org/10.1007/s41779-018-0220-5
- 9.Paliwal, P., Chauhan, G., Gautam, D., Dash, D., Patne, S.C., Krishnamurthy, S.: Indole-3-carbinol improves neurobehavioral symptoms in a cerebral ischemic stroke model. Naunyn Schmiedeberg's Arch Pharmacol. https://doi.org/10.1007/s00210-018-1488-2
- 12.Kumar, G., Paliwal, P., Mukherjee, S., Patnaik, N., Krishnamurthy, S., Patnaik, R.: Pharmacokinetics and brain penetration study of chlorogenic acid in rats. Xenobiotica. https://doi.org/10.1080/00498254.2018.1445882
- 13.Shakya, M., Paliwal, P., Patil, S., Koti, B.C., Swamy, A.H.: Cardioprotective effect of 'Qolest' a polyherbal formulation against doxorubicin induced cardiotoxicity in Wistar rats. Int J Res Pharm Sci. 1(3), 85–100 (2011)Google Scholar
- 16.Papadimitropoulos, A., Mastrogiacomo, M., Peyrin, F., Molinari, E., Komlev, V.S., Rustichelli, F., Cancedda, R.: Kinetics of in vivo bone deposition by bone marrow stromal cells within a resorbable porous calcium phosphate scaffold: an X-ray computed microtomography study. Biotechnol Bioeng. 98(1), 271–281 (2007)CrossRefGoogle Scholar