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Morphology, characterization, and conversion of the corals Goniopora spp. and Porites cylindrica to hydroxyapatite

  • S. AkyolEmail author
  • B. Ben Nissan
  • I. Karacan
  • M. Yetmez
  • H. Gokce
  • D. J. Suggett
  • F. N. Oktar
Research
  • 24 Downloads

Abstract

The aim of this study is to obtain pure natural hydroxyapatite (HAp) and tricalcium phosphate (TCP) from a Goniopora spp. and from hump coral (Porites cylindrica), both sourced from Australia. Due to the nature of the conversion process, commercial coralline HAp has retained coral or CaCO3, and the structure possesses both nano- and mesopores within the interpore trabeculae resulting in high dissolution rates. To overcome these limitations, a newly patented coral double-conversion technique has been developed. The current technique involves a two-stage application route where in the first-stage complete conversion of coral to pure HAp is achieved. In the second stage, a sol-gel-derived HAp nanocoating is directly applied to cover the meso- and nanopores within the intrapore material, while maintaining the large pores. Here, we specifically investigated the morphological changes and characterized these corals prior to and after conversion. For this purpose, four groups designated as C0, C1, C2, and C3 were used. C0 is Porites, Goniopora, and cylindrica; the original coral is calcium carbonate with aragonite structure that contains proteins and polysaccharides. C1 is coral cleaned under ultrasound in bleach diluted with water. C2 is coral converted to hydroxyapatite (HAp) by hydrothermal treatment method at 200 °C under pressure in the presence of ammonium biphosphate. C3 is obtained by coating C2 with sol-gel alkoxide-derived nanohydroxyapatite to obtain a more bioactive osteoconductive material and improve mechanical properties. All groups were characterized by XRD, EDAX, DTA/TGA, and SEM. The results showed that the biaxial strengths of the C2 and C3 were significantly higher than the original coral. The work also showed the advantages of the hydrothermal conversion method and the effect of the nanocoating which is expected to improve the final bioactivity through microstructural changes of the surfaces.

Keywords

Corals Aragonite Natural bioceramics Ultrasonication Hydrothermal treatment 

Notes

References

  1. 1.
    Ben-Nissan, B.: Natural bioceramics: from coral to bone and beyond. Curr Opinion Solid State Mater Sci. 7(4–5), 283–288 (2003)CrossRefGoogle Scholar
  2. 2.
    Macha, I.J., Ozyegin, L.S., Oktar, F.N., Ben-Nissan, B.: Conversion of ostrich eggshells (Struthio camelus) to calcium phosphates. J Aust Ceram Soc. 51(1), 125–133 (2015)Google Scholar
  3. 3.
    Kel, D., Gökçe, H., Bilgiç, D., Ağaoğulları, D., Duman, I., Öveçoğlu, M.L., Kayalı, E.S., Kiyici, I.A., Agathopoulos, S., Oktar, F.N.: Production of natural bioceramic from land snails. Key Eng Mater. 493-494, 287–292 (2012)CrossRefGoogle Scholar
  4. 4.
    Ozyegin, L.S., Sima, F., Ristoscu, C., Kiyici, I.A., Mihailescu, I.N., Meydanoglu, O., Agathopoulos, S., Oktar, F.N.: Sea snail: an alternative source for nano-bioceramic production. Key Eng Mater. 493-494, 781–786 (2012)CrossRefGoogle Scholar
  5. 5.
    Rocha, J.H.G., Lemos, A.F., Agathopoulos, S., Valério, P., Kannan, S., Oktar, F.N., Ferreira, J.M.F.: Scaffolds for bone restoration from cuttlefish. Bone. 37(6), 850–857 (2005)CrossRefGoogle Scholar
  6. 6.
    Agaogulları, D., Kel, D., Gokce, H., Duman, I., Öveçoğlu, M.L., Akarsubasi, A.T., Bilgic, D., Oktar, F.N.: Bioceramic production from sea urchins. Acta Phys Pol A. 121(1), 23–26 (2012)CrossRefGoogle Scholar
  7. 7.
    Samur, R., Ozyegin, L.S., Agaogullari, D., Oktar, F.N., Agathopoulos, S., Kalkandelen, C., Duman, I., Ben-Nissan, B.: Calcium phosphate formation from sea urchine (brissus latecarinatus) via modified mechano-chemical (ultrasonic) conversation method. Metal. 52, 375–378 (2013)Google Scholar
  8. 8.
    Karacan, I., Gunduz, O., Ozyegin, L.S., Gökce, H., Ben-Nissan, B., Akyol, S., Oktar, F.N.: The natural nano-bioceramic powder production from organ pipe red coral (tubipora musica) by a simple chemical conversion method. J Aust Ceram Soc. 54(2), 317–329 (2018)CrossRefGoogle Scholar
  9. 9.
    Oktar, F.N., Gokce, H., Gunduz, O., Sahin, Y.M., Agaogullari, D., Turner, I.G., Ozyegin, L.S., Ben-Nissan, B.: Bioceramic production from giant purple barnacle (megabalanus tintinnabulum). Key Eng Mater. 631, 137–142 (2015)CrossRefGoogle Scholar
  10. 10.
    Green, D.W., Ben-Nissan, B., Yoon, K.S., Milthorpe, B., Jung, H.S.: Bioinspired materials for regenerative medicine: going beyond the human archetypes. J Mater Chem B. 4(14), 2396–2406 (2016)CrossRefGoogle Scholar
  11. 11.
    Laine, J., Labady, M., Albornoz, A., Yunes, S.: Porosities and pore sizes in coralline calcium carbonate. Mater Charact. 59(10), 1522–1525 (2008)CrossRefGoogle Scholar
  12. 12.
    Roy, D., Linnehan, S.: Hydroxyapatite formed from coral skeletal carbonate by hydrothermal exchange. Nature. 247(438), 220–222 (1974)CrossRefGoogle Scholar
  13. 13.
    Hing, K.A., Best, S.M., Tanner, K.E., Bonfield, W., Revell, P.A.: Quantification of bone ingrowth within bone-derived porous hydroxyapatite implants of varying density. J Mater Sci Mater Med. 10(10/11), 663–670 (1999)CrossRefGoogle Scholar
  14. 14.
    Ben-Nissan B.: Discovery and development of marine biomaterials, In Functional Marine Biomaterials, Properties and Applications, Chapter1, Edited by Se-Kwon Kim, Woodhead Publishing, Print Book ISBN : 9781782420866, 3–32 (2015)Google Scholar
  15. 15.
    Chou, J., Valenzuela, S., Bishop, D., Ben-Nissan, B., Milthorpe, B.: Strontium- and magnesium-enriched biomimetic beta-TCP macrospheres with potential for bone tissue morphogenesis. J Tissue Eng Regen Med. 8(10), (2012)Google Scholar
  16. 16.
    Chai, C.S., Ben-Nissan, B.: Bioactive nanocrystalline sol-gel hydroxyapatite coatings. J Mater Sci Mater Med. 10(8), 465–469 (1999)CrossRefGoogle Scholar
  17. 17.
    Gross, K.A., Chai, C.S., Kannangara, G.S.K., Ben-Nissan, B., Hanley, L.: Thin hydroxyapatite coatings via sol–gel synthesis. J Mater Sci Mater Med. 9(12), 839–843 (1998)CrossRefGoogle Scholar
  18. 18.
    Ben-Nissan, B., Choi, A.H.: Sol-gel production of bioactive nanocoatings for medical applications. Part 1: an introduction. Future Medicine Ltd. 1(3), 311–319 (2006)Google Scholar
  19. 19.
    Zreiqat, H., Valenzuela, S.M., Ben-Nissan, B., Roest, R., Knabe, C., Radlanski, R.J.: The effect of surface chemistry modification of titanium alloy on signaling pathways in human osteoblasts. Biomaterials. 26(36), 7579–7586 (2005)CrossRefGoogle Scholar
  20. 20.
    Elsinger, E.C., Leal, L.: Coralline hydroxyapatite bone graft substitutes. The Journal of Foot and Ankle Surgery. 35(5) (9–10), 396–399 (1996)CrossRefGoogle Scholar
  21. 21.
    Songer, M., Baskin, D., Kabins, M., Reynolds, A., Zak, P.: Prospective randomized comparison of ProOsteon 200 Coralline Hydroxyapatite bone graft substitute versus iliac crest autograft in anterior cervical fusions. Spine J. 2(5), 64–64 (2002)CrossRefGoogle Scholar
  22. 22.
    Markel, D.C., Guthrie, S.T., Wu, B., Song, Z., Wooley, P.H.: Characterization of the inflammatory response to four commercial bone graft substitutes using a murine biocompatibility model. J Inflamm Res. 2012, 13–18 (2018)Google Scholar

Copyright information

© Australian Ceramic Society 2019

Authors and Affiliations

  1. 1.Physiology Department, Cerrahpasa Medical FacultyIstanbul UniversityIstanbulTurkey
  2. 2.School of Life SciencesUniversity of TechnologySydneyAustralia
  3. 3.Mechanical Engineering Department, Engineering FacultyZonguldak Bulent Ecevit UniversityZonguldakTurkey
  4. 4.Adnan Tekin CenterIstanbul Technical UniversityIstanbulTurkey
  5. 5.Climate Change Cluster (C3)University of TechnologySydneyAustralia
  6. 6.Bioengineering Department, Engineering FacultyMarmara UniversityIstanbulTurkey
  7. 7.Nanotechnology and Biomaterial Research and Implementation CentreMarmara UniversityIstanbulTurkey

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