Bioaccumulation of Mercury in the Copepod Pseudodiaptomus marinus: A Comparative Study Between Waterborne and Dietary Pathways
The fate and the accumulation kinetics of mercurychloride (HgCl2) were investigated in the invasive copepod species Pseudodiaptomus marinus, which originates from the North-Western Pacific Ocean and was recently recorded from the Atlantic and Mediterranean coasts. The main objective of this study was to determine lethal concentrations (LC50 %) of HgCl2 in P. marinus and to study its bioaccumulation kinetics in the laboratory. Lethality experiments were performed for 24, 48, 72, and 96 h. Experiments in presence and absence of food source using one sub-lethal concentration of HgCl2 (14.15 μg/L) were carried out to study the uptake, the accumulation and the influence of exposure pathways of HgCl2 in P. marinus. LC50 for 96 h was calculated as 42.4 μg/L in response to HgCl2. The uptake and bioaccumulation kinetics of HgCl2 in P. marinus are not depending on the exposure pathways, where no significant differences were depicted between the uptake/accumulation of HgCl2 from the micro-algal diet and from the seawater medium. Those results could be helpful in the understanding of mercury uptake, bioaccumulation and bio-amplification processes especially concerning invasive copepod species.
LC50 for 96h was calculated as 42.4 μg/L HgCl2.
I. galbana uptaked and accumulated Hg more than starved and fed P. marinus.
The uptake and the bioaccumulation kinetics of Hg was slow during the 1st and 4th days in starved and fed P. marinus.
The uptake and bioaccumulation kinetics of HgCl2 in P. marinus were not depending on the exposure pathway.
KeywordsBioaccumulation Mercury Pseudodiaptomus marinus Uptake kinetics Isochrysis galbana
This work is a contribution to the Interdisciplinary Environmental Institute of Lille 1 University (IREPSE) as well as to Lille 1 Grant (BQR-Convergence-2014) aiming to reinforce multidisciplinary studies around copepods. This study was funded by an Erasmus Mundus (Fatima Al Fihri Lot 2) Post-Doctoral fellowship to S. Tlili. We would like to thank LASIR and LOG teams (University of Lille, France) for facilities and help in performing experiments and analysis. Authors thank reviewers for valuable suggestions and recommendations.
- Barka S, Pavillon JF, Amiard JC (2001) Influence of different essential and non-essential metals on MTLP levels in the copepod Tigriopus brevicornis. Comp Biochem Physiol 128C:479–493Google Scholar
- Brylinski JM, Antajan E, Raud T, Vincent D (2012) First record of the Asian copepod Pseudodiaptomus marinus Sato, 1913 (Copepoda: Calanoida: Pseudodiaptomidae) in the southern bight of the North Sea along the coast of France. Aquat Invasions 7:577–584. https://doi.org/10.3391/ai.2012.7.4.014 CrossRefGoogle Scholar
- Daye M, Ouddane B, Halwani J, Hamzeh M (2013) Solid phase extraction of inorganic mercury using 5-phenylazo-8-hydroxyquinoline and determination by cold vapor atomic fluorescence spectroscopy in natural water samples. Sci World J. https://doi.org/10.1155/2013/134565
- Deschutter Y, Vergara G, Mortelmans J, Deneudt K, De Schamphelaere K, De Troch M (2018) Distribution of the invasive calanoid copepod Pseudodiaptomus marinus (Sato, 1913) in the Belgian part of the North Sea. BioInvasions Records 7:33–41. https://doi.org/10.3391/bir.2018.7.1.05 CrossRefGoogle Scholar
- Kidd KA, Muir DCG, Evans MS, Wang X, Whittle M, Swanson HK, Johnston T, Guildford S (2012) Biomagnification of mercury through lake trout (Salvelinus namaycush) food webs of lake with different physical, chemical, and biological characteristics. Sci Tot Environ 135:430–438Google Scholar
- Lassus P, Lebaut C, Le Dean L, Bardouil M, Truquet P, Bocquené G (1984) The use of harpacticoid copepods for testing the effects of chemicals on larval production. Inst Mar Sci Res 2:131–142Google Scholar
- Sabia L, Uttieri M, Pansera M, Souissi S, Schmitt FG, Zagami G, Zambianchi E (2012) First observations on the swimming behaviour of Pseudodiaptomus marinus from Lake Faro. Biol Mar Medit 19:240–241Google Scholar
- Sato F (1913) Pelagic copepods (no. 1). Sci Rep Hokkaido Fish Exp Stn 1:1–79Google Scholar
- Steele J H (1974) The structure of marine ecosystems. Harvard University Press, Cambridge. https://doi.org/10.4319/lo.1922.214.171.1245
- U.S. Environmental Protection Agency (1981) Interlaboratory comparison acute toxicity tests using estuarine animals. EPA-600/4-81/003. Washington, DCGoogle Scholar
- Uye S, Kasahara S (1983) Grazing of various developmental stages of Pseudodipatomus marinus (Copepoda: Calanoida) on natural occurring particles. Bull Plankton Soc Japan 30:147–158Google Scholar
- Uye S, Onbe T (1975) The developmental stages of Pseudodiaptomus marinus SATO (Copepoda: Calanoida) reared in the laboratory. Bull Plankton Soc Japan 21:65–76Google Scholar
- Williams JJ, Dutton J, Chen CY, Fisher NS (2010) Metal (As, Cd, Hg, and CH3Hg) bioaccumulation from water and food by the benthic amphipod Leptocheirus plumulosus. Environ Toxicol Chem 29:1755–1761Google Scholar
- Zidour M, Boubechiche Z, Pan YJ, Bialais C, Cudennec B, Grard T, Drider D, Flahaut C, Ouddane B, Souissi S (2019). Population response of the estuarine copepod Eurytemora affinis to its bioaccumulation of trace metals. Chemosphere 220:505–513. https://doi.org/10.1016/j.chemosphere.2018.12.148 CrossRefGoogle Scholar