Improvements in metal exposure assays: artificial food to assess bioaccumulation in the blue crab Callinectes danae Smith, 1869 (Crustacea, Decapoda, Portunidae)

  • Isabella C. BordonEmail author
  • Bruno Galvão de Campos
  • Paloma Kachel Gusso-Choueri
  • Caio Akira Miyai
  • Giuliana Seraphim de Araujo
  • Andrews Krupinski Emerenciano
  • José Roberto Machado Cunha da Silva
  • Marycel Elena Barboza Cotrim
  • Déborah Inês Teixeira Favaro
  • Denis Moledo de Souza Abessa
Technical note


This technical note aims to report, in detail, the development, suitability and analytical validation of an artificial food to assess metal bioaccumulation in the blue crab Callinectes danae after metal exposure assays. For this purpose, lead (Pb) was chosen as the metal of interest. First, a non-contaminated artificial food was prepared by mixing commercial shrimps and agarose. The food was then offered on alternate days to two groups of crabs for 7 and 14 days, respectively. After confirming that this food was well-accepted by crabs, analytical procedures were performed to confirm that Pb added to the mixture would remain in the artificial food. For this purpose, two foods containing Pb at concentrations of 0.5 and 2.0 µg g−1 were prepared by adding aliquots of an ultrapure Pb standard solution to the mixture. Samples of each food were subjected to an acid extraction and Pb concentrations were determined by a GF-AAS. Pb recoveries in both contaminated foods were > 90%. Subsequently, another experiment was performed, and two groups of crabs were fed with contaminated artificial foods (0.5 and 2.0 µg g−1 of Pb) during 7 and 14 days, respectively. The observed acceptance of these foods by crabs at both Pb concentrations was of 100%.

Article Highlights

  • An artificial food was prepared to assess bioaccumulation in the blue crab

  • Lead (Pb) was chosen as the metal of interest

  • The acceptance of artificial food was evaluated

  • In the contaminated artificial foods, Pb was determined

  • It was concluded that this food can be applied in metal exposure assays


Artificial food Crab Callinectes Pb Exposure Metal 



The authors would like to thank FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) for the financial support (Processo FAPESP n. 2014/01576–6).

Conflict of interest

The authors declare no conflict of interest.

Ethical statement

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.


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Copyright information

© University of Tehran 2019

Authors and Affiliations

  • Isabella C. Bordon
    • 1
    Email author
  • Bruno Galvão de Campos
    • 1
  • Paloma Kachel Gusso-Choueri
    • 1
  • Caio Akira Miyai
    • 2
  • Giuliana Seraphim de Araujo
    • 1
  • Andrews Krupinski Emerenciano
    • 3
  • José Roberto Machado Cunha da Silva
    • 3
  • Marycel Elena Barboza Cotrim
    • 4
  • Déborah Inês Teixeira Favaro
    • 5
  • Denis Moledo de Souza Abessa
    • 1
  1. 1.Núcleo de Estudos em Poluição e Ecotoxicologia Aquática (NEPEA)UNESP-Univ Estadual PaulistaSão VicenteBrazil
  2. 2.Laboratório de Ecologia e Comportamento Animal (Labecom)UNESP-Univ Estadual PaulistaSão VicenteBrazil
  3. 3.Biomedical and Biological Sciences Institute (ICB)USP- University of São PauloSão PauloBrazil
  4. 4.Chemical and Environmental Technology CentreIPEN- Nuclear and Energy Research InstituteSao PauloBrazil
  5. 5.Research Reactor Centre, IPEN- Nuclear and Energy Research InstituteSao PauloBrazil

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