Effects of Copper and PQQ on the Denitrification Activities of Microorganisms Facilitating Nitrite- and Nitrate-Dependent DAMO Reaction
The growth rate of microorganisms that facilitate the denitrifying anaerobic methane oxidation (DAMO) reaction is slow; thus, establishment of appropriate cultivation conditions for these microorganisms is necessary. In this study, the effect of copper (Cu) and pyrroloquinoline quinone (PQQ) concentrations on the nitrite- and nitrate-dependent DAMO activities of these microorganisms were evaluated using an upflow-type continuous cultivation system. The study confirmed that 6 µM Cu and 2 nM PQQ had a positive effect on nitrite-dependent DAMO, but had little effect on nitrate-dependent DAMO. Conventional basal medium does not contain PQQ; therefore, supplementation of basal medium with PQQ could be an effective method for the cultivation of bacteria that facilitate nitrite-dependent DAMO.
KeywordsM. oxyfera M. nitroreducens NC10 Anaerobic methanotroph
This research was financially supported by JSPS KAKENHI Grant Numbers 25701010, 15K12225, and 16H02975.
- Ameyama M, Shinagawa E, Matsushita K, Adachi O (1985) Growth stimulating activity for microorganisms in naturally occurring substances and partial characterization of the substance for the activity as pyrroloquinoline quinone. Agric Biol Chem 49:699–709Google Scholar
- Ettwig KF, Butler MK, Le Paslier D, Pelletier E, Mangenot S, Kuypers MMM, Schreiber F, Dutilh BE, Zedelius J, De Beer D, Gloerich J, Wessels HJCT, Van Alen T, Luesken F, Wu ML, Van De Pas-Schoonen KT, Op Den Camp HJM, Janssen-Megens EM, Francoijs KJ, Stunnenberg H, Weissenbach J, Jetten MSM, Strous M (2010) Nitrite-driven anaerobic methane oxidation by oxygenic bacteria. Nature 464:543–548CrossRefGoogle Scholar
- Forster P, Ramaswamy V, Artaxo P, Berntsen T, Betts R, Fahey DW, Haywood J, Lean J, Lowe DC, Myhre G, Nganga J, Prinn R, Raga G, Schulz M, Van Dorland R (2007) Changes in atmospheric constituents and in radiative forcing. In: Solomon S, Qin D, Manning M, Chen Z, Marquis M, Averyt KB, Tignor M, Miller HL (eds) Climate change 2007: the physical science basis. Contribution of working group I to the fourth assessment report of the intergovernmental panel on climate change. Cambridge University Press, Cambridge, New YorkGoogle Scholar
- Glass JB, Orphan VJ (2012) Trace metal requirements for microbial enzymes involved in the production and consumption of methane and nitrous oxide. Front Microbiol 3:61Google Scholar
- He Z, Wang J, Zhang X, Cai C, Geng S, Zheng P, Xu X, Hu B (2015c) Nitrogen removal from wastewater by anaerobic methane-driven denitrification in a lab-scale reactor: heterotrophic denitrifiers associated with denitrifying methanotrophs. Appl Microbiol Biotechnol 99:10853–10860CrossRefGoogle Scholar
- Luesken FA, Van Alen TA, Van Der Biezen E, Frijters C, Toonen G, Kampman C, Hendrickx TLG, Zeeman G, Temmink H, Strous M, Op Den Camp HJM, Jetten MSM (2011) Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge. Appl Microbiol Biotechnol 92:845–854CrossRefGoogle Scholar