Advertisement

Journal of Analysis and Testing

, Volume 3, Issue 3, pp 269–276 | Cite as

Bivalent Metal Ions Tethered Fluorescent Gold Nanoparticles as a Reusable Peroxidase Mimic Nanozyme

  • Liu Liu
  • Hui JiangEmail author
  • Xuemei WangEmail author
Original Paper
  • 78 Downloads

Abstract

Nanozymes are a class of mimic enzymes that have both unique properties of nanomaterials and catalytic functions. They are widely used in medicine, food, agriculture, and environment due to their high catalytic efficiency, stability, economy, and large-scale preparation. In this work, a simple, gentle method was applied to synthesize adenosine phosphates (AXP) templated gold nanoparticles (Au NPs) with small size, good optical properties, and peroxidase-like activities. In the presence of hydrogen peroxide (H2O2), the peroxidase substrate 3, 3′, 5, 5′-tetramethylbenzidine (TMB) could be oxidized to form blue-colored oxidized TMB (oxTMB), which demonstrates that the AuAXP NPs have peroxidase-like activities. Interestingly, the introduction of bivalent metal ions into AuAXP NPs allows their reusability. Among them, after three rounds of recycling, the catalytic activity of Mg@AuAMP could still reach 92.69% compared to the original level, which showed excellent reusability. In addition, the glutathione (GSH) detection was realized with a linear detection range of 0–200 μM due to the GSH-induced loss of peroxidase-like activities. Overall, this work provides a new idea of recycling for biosensor and catalytic analysis.

Keywords

AXP-mimic peroxidase Reusability H2O2 GSH 

Notes

Funding

This study was funded by National Natural Science Foundation of China (21675023, 91753106, 21828501), Key Technologies Research and Development Program (2017YFA0205300) and Natural Science Foundation of Jiangsu (BK20161413).

Supplementary material

41664_2019_109_MOESM1_ESM.docx (290 kb)
Supplementary material 1 (DOCX 289 kb)

References

  1. 1.
    Huang Y, Zhao M, Han S, et al. Growth of Au nanoparticles on 2D metalloporphyrinic metal-organic framework nanosheets used as biomimetic catalysts for cascade reactions. Adv Mater. 2017;29:1700102.CrossRefGoogle Scholar
  2. 2.
    Sun H, Zhou Y, Ren J, et al. Carbon nanozymes: enzymatic properties, catalytic mechanism, and applications. Angewandte Chemie-Int Edn. 2018;57:9224.CrossRefGoogle Scholar
  3. 3.
    Gao L, Zhuang J, Nie L, et al. Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol. 2007;2:577.CrossRefPubMedGoogle Scholar
  4. 4.
    Herget K, Frerichs H, Pfitzner F, et al. Functional enzyme mimics for oxidative halogenation reactions that combat biofilm formation. Adv Mater. 2018;30:1707073.CrossRefGoogle Scholar
  5. 5.
    Pedone D, Moglianetti M, De Luca E, et al. Platinum nanoparticles in nanobiomedicine. Chem Soc Rev. 2017;46:4951.PubMedCrossRefGoogle Scholar
  6. 6.
    Wu J, Li S, Wei H. Integrated nanozymes: facile preparation and biomedical applications. Chem Commun. 2018;54:6520.CrossRefGoogle Scholar
  7. 7.
    Zhu W, Zhang J, Jiang Z, et al. High-quality carbon dots: synthesis, peroxidase-like activity and their application in the detection of H2O2, Ag+ and Fe3+. Rsc Adv. 2014;4:17387.CrossRefGoogle Scholar
  8. 8.
    Kluenker M, Tahir MN, Ragg R, et al. Pd@Fe2O3 superparticles with enhanced peroxidase activity by solution phase epitaxial growth. Chem Mater. 2017;29:1134.CrossRefGoogle Scholar
  9. 9.
    Wu YH, Chu L, Liu W, et al. The screening of metal ion inhibitors for glucose oxidase based on the peroxidase-like activity of nano-Fe3O4. Rsc Adv. 2017;7:47309.CrossRefGoogle Scholar
  10. 10.
    Yang Q, Lu S, Shen B, et al. An iron hydroxyl phosphate microoctahedron catalyst as an efficient peroxidase mimic for sensitive and colorimetric quantification of H2O2 and glucose. New J Chem. 2018;42:6803.CrossRefGoogle Scholar
  11. 11.
    Bao YW, Hua XW, Ran HH, et al. Metal-doped carbon nanoparticles with intrinsic peroxidase-like activity for colorimetric detection of H2O2 and glucose. J Mater Chem B. 2019;7:296.CrossRefGoogle Scholar
  12. 12.
    Ren X, Meng X, Ren J, et al. Graphitic carbon nitride nanosheets with tunable optical properties and their superoxide dismutase mimetic ability. Rsc Adv. 2016;6:92839.CrossRefGoogle Scholar
  13. 13.
    Vazquez-Gonzalez M, Liao WC, Gazelles R, et al. Mimicking horseradish peroxidase functions using Cu2+-modified carbon nitride nanoparticles or Cu2+-modified carbon dots as heterogeneous catalysts. ACS Nano. 2017;11:3247.PubMedCrossRefGoogle Scholar
  14. 14.
    Wang H, Li P, Yu D, et al. Unraveling the enzymatic activity of oxygenated carbon nanotubes and their application in the treatment of bacterial infections. Nano Lett. 2018;18:1530.CrossRefGoogle Scholar
  15. 15.
    Islamoglu T, Atilgan A, Moon S-Y, et al. Cerium(IV) vs Zirconium(IV) based metal-organic frameworks for detoxification of a nerve agent. Chem Mater. 2017;29:2672.CrossRefGoogle Scholar
  16. 16.
    Wang Y, Zhu Y, Binyam A, et al. Discovering the enzyme mimetic activity of metal-organic framework (MOF) for label-free and colorimetric sensing of biomolecules. Biosens Bioelectron. 2016;86:432.PubMedCrossRefGoogle Scholar
  17. 17.
    Drozd M, Pietrzak M, Parzuchowski PG, et al. Pitfalls and capabilities of various hydrogen donors in evaluation of peroxidase-like activity of gold nanoparticles. Anal Bioanal Chem. 2016;408:8505.PubMedPubMedCentralCrossRefGoogle Scholar
  18. 18.
    Li K, Wang K, Qin W, et al. DNA-directed assembly of gold nanohalo for quantitative plasmonic imaging of single-particle catalysis. J Am Chem Soc. 2015;137:4292.PubMedCrossRefGoogle Scholar
  19. 19.
    Zhu X, Mao X, Wang Z, et al. Fabrication of nanozyme@DNA hydrogel and its application in biomedical analysis. Nano Res. 2017;10:959.CrossRefGoogle Scholar
  20. 20.
    Jiang T, Song Y, Wei T, et al. Sensitive detection of Escherichia coli O157:H7 using Pt–Au bimetal nanoparticles with peroxidase-like amplification. Biosens Bioelectron. 2016;77:687.PubMedCrossRefGoogle Scholar
  21. 21.
    Jin L, Meng Z, Zhang Y, et al. Ultrasmall Pt nanoclusters as robust peroxidase mimics for colorimetric detection of glucose in human serum. ACS Appl Mater Interfaces. 2017;9:10027.PubMedCrossRefGoogle Scholar
  22. 22.
    Karim MN, Anderson SR, Singh S, et al. Nanostructured silver fabric as a free-standing nanozyme for colorimetric detection of glucose in urine. Biosens Bioelectron. 2018;110:8.CrossRefPubMedGoogle Scholar
  23. 23.
    Liu Y, Purich DL, Wu C, et al. Ionic functionalization of hydrophobic colloidal nanoparticles to form ionic nanoparticles with enzyme like properties. J Am Chem Soc. 2015;137:14952.PubMedPubMedCentralCrossRefGoogle Scholar
  24. 24.
    Sloan-Dennison S, Laing S, Shand NC, et al. A novel nanozyme assay utilising the catalytic activity of silver nanoparticles and SERRS. Analyst. 2017;142:2484.PubMedCrossRefGoogle Scholar
  25. 25.
    Yang L, Liu X, Lu Q, et al. Catalytic and peroxidase-like activity of carbon based-AuPd bimetallic nanocomposite produced using carbon dots as the reductant. Anal Chim Acta. 2016;930:23.PubMedCrossRefGoogle Scholar
  26. 26.
    Chen K, Bayaguud A, Li H, et al. Improved peroxidase-mimic property: sustainable, high-efficiency interfacial catalysis with H2O2 on the surface of vesicles of hexavanadate-organic hybrid surfactants. Nano Res. 2018;11:1313.CrossRefGoogle Scholar
  27. 27.
    Chen TM, Xiao J, Yang GW. Cubic boron nitride with an intrinsic peroxidase-like activity. Rsc Adv. 2016;6:70124.CrossRefGoogle Scholar
  28. 28.
    Li YZ, Li TT, Chen W, et al. Co4N Nanowires: noble-metal-free peroxidase mimetic with excellent salt- and temperature-resistant abilities. ACS Appl Mater Interfaces. 2017;9:29881.PubMedCrossRefGoogle Scholar
  29. 29.
    Thawari AG, Rao CP. Peroxidase-like catalytic activity of copper-mediated protein-inorganic hybrid nanoflowers and nanofibers of beta-Lactoglobulin and alpha-Lactalbumin: synthesis, spectral characterization, microscopic features, and catalytic activity. ACS Appl Mater Interfaces. 2016;8:10392.PubMedCrossRefGoogle Scholar
  30. 30.
    Weerathunge P, Ramanathan R, Shukla R, et al. Aptamer-controlled reversible inhibition of gold nanozyme activity for pesticide sensing. Anal Chem. 2014;86:11937.PubMedCrossRefGoogle Scholar
  31. 31.
    Drozd M, Pietrzak M, Parzuchowski P, et al. Peroxidase-like activity of gold nanoparticles stabilized by hyperbranched polyglycidol derivatives over a wide pH range. Nanotechnology. 2015;26:495101.PubMedCrossRefGoogle Scholar
  32. 32.
    Singh R, Belgamwar R, Dhiman M, et al. Dendritic fibrous nano-silica supported gold nanoparticles as an artificial enzyme. J Mater Chem B. 2018;6:1600.CrossRefGoogle Scholar
  33. 33.
    Jiang H, Zhang YY, Wang XM. Single cytidine units-templated syntheses of multi-colored water-soluble Au nanoclusters. Nanoscale. 2014;6(17):10355.PubMedCrossRefGoogle Scholar
  34. 34.
    Lopez A, Liu JW. Light-activated metal-coordinated supramolecular complexes with charge-directed self-assembly. J Phys Chem C. 2013;117(7):3653–61.CrossRefGoogle Scholar
  35. 35.
    Chen Q, Liu M, Zhao J, et al. Water-dispersible silicon dots as a peroxidase mimetic for the highly-sensitive colorimetric detection of glucose. Chem Commun. 2014;50:6771.CrossRefGoogle Scholar
  36. 36.
    Jang GG, Roper DK. Balancing redox activity allowing spectrophotometric detection of Au(I) Using tetramethylbenzidine dihydrochloride. Anal Chem. 2011;83:1836.PubMedCrossRefGoogle Scholar
  37. 37.
    Wang GL, Jin LY, Dong YM, et al. Intrinsic enzyme mimicking activity of gold nanoclusters upon visible light triggering and its application for colorimetric trypsin detection. Biosens Bioelectron. 2015;64:523.PubMedCrossRefGoogle Scholar
  38. 38.
    Jv Y, Li B, Cao R. Positively-charged gold nanoparticles as peroxidiase mimic and their application in hydrogen peroxide and glucose detection. Chem Commun. 2010;46:8017.CrossRefGoogle Scholar
  39. 39.
    Li M, Yang J, Ou Y, et al. Peroxidase-like activity of 2 ‘,7 ‘-difluorofluorescein and its application for galactose detection. Talanta. 2018;182:422.PubMedCrossRefGoogle Scholar
  40. 40.
    Mu J, Wang Y, Zhao M, et al. Intrinsic peroxidase-like activity and catalase-like activity of Co3O4 nanoparticles. Chem Commun. 2012;48:2540.CrossRefGoogle Scholar
  41. 41.
    Song Y, Qu K, Zhao C, et al. Graphene oxide: intrinsic peroxidase catalytic activity and its application to glucose detection. Adv Mater. 2010;22:2206.PubMedCrossRefGoogle Scholar

Copyright information

© The Nonferrous Metals Society of China 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Bioelectronics, School of Biological Science and Medical EngineeringSoutheast UniversityNanjingPeople’s Republic of China

Personalised recommendations