Journal of Analysis and Testing

, Volume 3, Issue 3, pp 269–276 | Cite as

Bivalent Metal Ions Tethered Fluorescent Gold Nanoparticles as a Reusable Peroxidase Mimic Nanozyme

  • Liu Liu
  • Hui JiangEmail author
  • Xuemei WangEmail author
Original Paper


Nanozymes are a class of mimic enzymes that have both unique properties of nanomaterials and catalytic functions. They are widely used in medicine, food, agriculture, and environment due to their high catalytic efficiency, stability, economy, and large-scale preparation. In this work, a simple, gentle method was applied to synthesize adenosine phosphates (AXP) templated gold nanoparticles (Au NPs) with small size, good optical properties, and peroxidase-like activities. In the presence of hydrogen peroxide (H2O2), the peroxidase substrate 3, 3′, 5, 5′-tetramethylbenzidine (TMB) could be oxidized to form blue-colored oxidized TMB (oxTMB), which demonstrates that the AuAXP NPs have peroxidase-like activities. Interestingly, the introduction of bivalent metal ions into AuAXP NPs allows their reusability. Among them, after three rounds of recycling, the catalytic activity of Mg@AuAMP could still reach 92.69% compared to the original level, which showed excellent reusability. In addition, the glutathione (GSH) detection was realized with a linear detection range of 0–200 μM due to the GSH-induced loss of peroxidase-like activities. Overall, this work provides a new idea of recycling for biosensor and catalytic analysis.


AXP-mimic peroxidase Reusability H2O2 GSH 



This study was funded by National Natural Science Foundation of China (21675023, 91753106, 21828501), Key Technologies Research and Development Program (2017YFA0205300) and Natural Science Foundation of Jiangsu (BK20161413).

Supplementary material

41664_2019_109_MOESM1_ESM.docx (290 kb)
Supplementary material 1 (DOCX 289 kb)


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Copyright information

© The Nonferrous Metals Society of China 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Bioelectronics, School of Biological Science and Medical EngineeringSoutheast UniversityNanjingPeople’s Republic of China

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