Journal of Analysis and Testing

, Volume 2, Issue 4, pp 352–355 | Cite as

Comparative Analysis of Polyphenolic and Antioxidant Constituents in Dried Seedlings and Seedless Acacia nilotica Fruits

  • Amani AbdErahman
  • Olalere Olusegun AbayomiEmail author
  • AbdElhafiz Eltahir Ahmed
  • Abdurahman Hamid NourEmail author
  • Rosli bin Mohd Yunus
  • Ghada Mohamed Ibrahim
  • Nassereldeen Ahmed Kabbashi
Rapid Communication


The phenolic and antioxidant constituents in Acacia nilotica fruits have become an important source of medicinal and therapeutic benefit with powerful biological properties. This study investigated the phenolic content and antioxidant capacity of powdered Acacia fruits with seeds and without seeds. The phenolic content and antioxidant capacities in them were determined using Folin–Ciocalteu and DPPH free radical-scavenging assays. The total phenolic and antioxidants of A. nilotica with seeds were spectrophotometrically determined to be 47.61 and 6.18% greater than when the seeds were removed from the dried fruits, respectively. The LC–MS/QTOF analysis shows the presence of 282 and 214 phenolic compounds in the methanol extracts of A. nilotica with seeds and without seeds, respectively. The present study, therefore, revealed that dried A. nilotica fruits with seeds have higher total phenolic content, antioxidant capacity, and bioactive constituents, which indicated that they have more medicinal value than fruits without seeds.


Acacia nilotica Liquid chromatography–mass spectrometry Total phenolic content DPPH antioxidant capacity 



We acknowledge the financial contribution of the Ministry of Higher Education, Malaysia, for their support through the FRGS (RDU-160148) research grants.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© The Nonferrous Metals Society of China 2018

Authors and Affiliations

  1. 1.Faculty of Science and TechnologyOmdurman Islamic UniversityOmdurmanSudan
  2. 2.Faculty of Chemical and Natural Resources EngineeringUniversiti Malaysia PahangGambangMalaysia
  3. 3.Faculty of Animal ProductionBahri UniversityBahriSudan
  4. 4.International Islamic University Malaysia, Malaysia, KOE, BTE EngineeringKuala LumpurMalaysia

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