The effect of antimicrobial photodynamic therapy mediated by papain gel on infected dentin in primary teeth: a clinical trial with microbiological evaluation

  • Larissa Costa-Santos
  • Ana Carolina Costa da Mota
  • Renato Araujo Prates
  • Lara Jansiski Motta
  • Anna Carolina Ratto Tempestini Horliana
  • Zenildo Santos Silva-Junior
  • Raquel Agnelli Mesquita-Ferrari
  • Kristianne Porta Santos Fernandes
  • Sandra Kalil BussadoriEmail author
Original Article



Antimicrobial photodynamic therapy (aPDT) has been used for treatment of dental caries. Papacarie™ is a gel composed of papain and chloramine employed for the partial removal of carious tissue, effective against bacteria; however, some studies report that this antibacterial action is not quite so evident. The aim of this study is to evaluate the clinical effect of the substance PapaMBlue followed by laser irradiation on infected dentin in dental caries lesion in primary teeth.


Thirty-two primary molars with deep occlusal dental caries were selected and divided in two groups: G1—caries removal with a low-speed drill—and G2—application of aPDT with PapacarieMBlue. After treatment, all the teeth were restored with glass ionomer cement and followed up clinically and radiographically, with evaluations at 3, 6, and 12 months. Dentin samples before and after treatment were analyzed microbiologically.


A reduction in the bacterial count was found in both groups and the inter-group difference was non-significant (p = 0.41022).


It is possible to conclude that there was a microbial reduction with the two treatments proposed; however, PapaMBlue is minimally invasive, maintains healthy tissue integrity, and is easy to use in Pediatric Dentistry and Public Health.

Trial registration

NCT02734420 on 10 March 2016


Dental caries Antimicrobial photodynamic therapy Low-level laser therapy 



The authors gratefully thank FAPESP process number 2016/11711-3 Nove de Julho University for its technological support.

Compliance with ethical standards

This clinical trial received approval from the Human Research Ethics Committee of University Nove de Julho(certificate number: 391563) .

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Larissa Costa-Santos
    • 1
  • Ana Carolina Costa da Mota
    • 1
  • Renato Araujo Prates
    • 1
  • Lara Jansiski Motta
    • 1
  • Anna Carolina Ratto Tempestini Horliana
    • 1
  • Zenildo Santos Silva-Junior
    • 1
  • Raquel Agnelli Mesquita-Ferrari
    • 1
  • Kristianne Porta Santos Fernandes
    • 1
  • Sandra Kalil Bussadori
    • 1
    Email author
  1. 1.Nove de Julho UniversitySão PauloBrazil

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