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Lasers in Dental Science

, Volume 2, Issue 4, pp 255–263 | Cite as

Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in rats

  • Paula Delello Macedo
  • Sâmara Tfaile Corbi
  • Fernanda Ali Kitagawa
  • Janice Rodrigues Perussi
  • Anderson Orzari Ribeiro
  • Rosemary Adriana Chierici Marcantonio
Original Article
  • 170 Downloads

Abstract

Purpose

The purpose of this study was to evaluate the effectiveness of a photosensitizer hypericin-glucamine, activated by LED amber (34.10 J/cm2) as an adjuvant to scaling and root planing (SRP), on the treatment of experimental periodontal disease (PD) in rats.

Methods

In a 15-day period, PD was induced in the mandibular molars through ligature placement. The animals (n = 60) were randomly divided into four groups: control (with induction of periodontal disease), scaling and root planing (SRP), antimicrobial photodynamic therapy (aPDT), and SRP + aPDT. At 7, 15, and 30 days after treatments, animals were euthanized. Digital microtomography, histometric, and stereometric analyses were performed to calculate the bone loss of mandibular second molars, and the tissue repair was analyzed histologically. The data were subjected to statistical analysis (α = 5%).

Results

Histologically, the control group periodontium showed several morphological changes, but an evident gradual reduction in the inflammatory process was observed in the subsequent periods. The SRP, aPDT, and SRP + aPDT groups presented the same, but in less intensity. The stereometric analysis showed a significantly higher proportion of fibroblasts in SRP group (p < 0,001) and SRP + aPDT group (p < 0.0001) compared to that in the control group at 7 days post treatment.

Conclusions

We conclude that scaling and root planing with or without photodynamic therapy with hypericin-glucamine increased density of the fibroblast and cell density. However, there is no statistically significant difference between SRP and SRP + aPDT.

Keywords

Hypericin-glucamine Scaling and root planing Periodontal disease 

Notes

Funding

This work was supported in part by a grant from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (BR) (AUXPG 655/2014) and by a grant from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (BR) (133436/2012-8).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Ethical board approval was obtained for this study by the Ethics in Animal Research Committee of the School of Dentistry of Araraquara (UNESP, Brazil CEEA/FOAr 07/2012).

References

  1. 1.
    Agostinis P, Vantieghem A, Merlevede W, de Witte PA (2002) Hypericin in cancer treatment: more light on the way. Int J Biochem Cell Biol 34:221–241.  https://doi.org/10.1016/S1357-2725(01)00126-1 CrossRefPubMedGoogle Scholar
  2. 2.
    Atieh MA (2010) Photodynamic therapy as an adjunctive treatment for chronic periodontitis: a meta-analysis. Lasers Med Sci 25:605–613.  https://doi.org/10.1007/s10103-009-0744-6 CrossRefPubMedGoogle Scholar
  3. 3.
    Azarpazhooh A, Shah PS, Tenenbaum HC, Goldberg MB (2010) The effect of photodynamic therapy for periodontitis: a systematic review and meta-analysis. J Periodontol 81:4–14.  https://doi.org/10.1902/jop.2009.090285 CrossRefPubMedGoogle Scholar
  4. 4.
    Bernal C, Tominagal TT, Ribeiro AO, Imasato H, Perussi JR (2011) Comparative studies of photophysical and biological properties of hypericin and hypericin-glucamine. Photodiagn Photodyn Ther 8:190–190.  https://doi.org/10.1016/j.pdpdt.2011.03.225 CrossRefGoogle Scholar
  5. 5.
    Braun A, Dehn C, Krause F, Jepsen S (2008) Short-term clinical effects of adjunctive antimicrobial photodynamic therapy in periodontal treatment: a randomized clinical trial. J Clin Periodontal 35:877–884.  https://doi.org/10.1111/j.1600-051X.2008.01303.x CrossRefGoogle Scholar
  6. 6.
    Cantley MD, Bartold PM, Marino V, Reid RC, Fairlie DP, Wyszynski RN, Zilm PS, Haynes DR (2009) The use of live-animal micro-computed tomography to determine the effect of a novel phospholipase A2 inhibitor on alveolar bone loss in an in vivo mouse model of periodontitis. J Periodontal Res 44:317–322.  https://doi.org/10.1111/j.1600-0765.2008.01132.x CrossRefPubMedGoogle Scholar
  7. 7.
    César Neto JB, Benatti BB, Sallum EA, Casati MZ, Nociti FH Jr (2006) The influence of cigarette smoke inhalation and its cessation on the tooth supporting alveolar bone: a histometric study in rats. J Periodontal Res 41:118–123.  https://doi.org/10.1111/j.1600-0765.2005.00844.x CrossRefPubMedGoogle Scholar
  8. 8.
    Checchi L, Montevecchi M, Gatto RM, Moreschi A, Checchi V (2007) Clinical efficacy of two toothbrushes with different bristles. Int J Dent Hyg 5:242–246.  https://doi.org/10.1111/j.1601-5037.2007.00258.x CrossRefPubMedGoogle Scholar
  9. 9.
    Chondros P, Nikolidakis D, Christodoulides N, Rossler R, Gutknecht N, Sculean A (2009) Photodynamic therapy as adjunct to nonsurgical periodontal treatment in patients on periodontal maintenance: a randomized controlled clinical trial. Lasers Med Sci 24:681–688.  https://doi.org/10.1007/s10103-008-0565-z CrossRefPubMedGoogle Scholar
  10. 10.
    Christodoulides N, Nikolidakis D, Chondros P, Becker J, Schwarz F, Rössler R, Sculean A (2008) Photodynamic therapy as an adjunct to non-surgical periodontal treatment: a randomized, controlled clinical trial. J Periodontol 79:1638–1644.  https://doi.org/10.1902/jop.2008.070652 CrossRefPubMedGoogle Scholar
  11. 11.
    Dai T, Huang YY, Hamblin MR (2009) Photodynamic therapy for localized infections – state of the art. Photodiagn Photodyn Ther 6:170–188.  https://doi.org/10.1016/j.pdpdt.2009.10.008 CrossRefGoogle Scholar
  12. 12.
    Drisko CH (1998) The use of locally-delivered doxycycline in the treatment of periodontitis. Clinical results. J Clin Periodontol 25:947–952.  https://doi.org/10.1111/j.1600-051X.1998.tb02396.x CrossRefPubMedGoogle Scholar
  13. 13.
    Franzò D, Philpotts CJ, Cox TF, Joiner A (2010) The effect of toothpaste concentration on enamel and dentine wear in vitro. J Dent 38:974–979.  https://doi.org/10.1016/j.jdent.2010.08.010 CrossRefPubMedGoogle Scholar
  14. 14.
    Garcia VG, Longo M, Fernandes LA, Gualberto EC Jr, Santinoni Cdos S, Bosco AF et al (2013) Treatment of experimental periodontitis in rats using repeated adjunctive antimicrobial photodynamic therapy. Lasers Med Sci 28:143–150.  https://doi.org/10.1007/s10103-012-1099-y CrossRefPubMedGoogle Scholar
  15. 15.
    Hayek RRA, Araújo NS, Gloso MA, Ferreira J, Baptista-Sobrinho CA, Yamada AM et al (2005) Comparative study between the effects of photodynamic therapy and conventional therapy on microbial reduction in ligature induced peri-implantitis in dogs. J Periodontol 76:1275–1281.  https://doi.org/10.1902/jop.2005.76.8.1275 CrossRefPubMedGoogle Scholar
  16. 16.
    Jori G, Fabris C, Soncin M, Ferro S, Coppellotti O, Dei D, Fantetti L, Chiti G, Roncucci G (2006) Photodynamic therapy in the treatment of microbial infections: basic principles and perspective applications. Lasers Surg Med 38:468–481.  https://doi.org/10.1002/lsm.20361 CrossRefPubMedGoogle Scholar
  17. 17.
    Karioti A, Bilia AR (2010) Hypericins as potential leads for new therapeutics. Int J Mol Sci 11(2):562–594.  https://doi.org/10.3390/ijms11020562 CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Klausen B (1991) Microbiological and immunological aspects of experimental periodontal disease in rats: a review article. J Periodontol 62:59–73.  https://doi.org/10.1902/jop.1991.62.1.59 CrossRefPubMedGoogle Scholar
  19. 19.
    Komerik N, Wilson M, Poole S (2000) The effect of photodynamic action on two virulence factors of gram negative bacteria. Photochem Photobiol 72:676–680.  https://doi.org/10.1562/0031-8655(2000)0720676TEOPAO2.0.CO2 CrossRefPubMedGoogle Scholar
  20. 20.
    Lodish H, ABerk A, Zipursky L, Matsudaira P, Baltimore D, Darnell J (2000) Collagen: the fibrous proteins of the matrix. In: Molecular cell biology, 4rd edn. W. H. Freeman, New York, pp 230–270Google Scholar
  21. 21.
    Macedo PD, Tsurumaki JN, Rossa-Junior C, Marcantonio RAC (2013) Quantificação de volume ósseo em microtomografia computadorizada: comparação entre diferentes áreas de interesse (ROI). Proceedings of the 30 th SBPqO Annual Meeting; 2013. Braz Oral Res 27(Suppl. 1):294Google Scholar
  22. 22.
    Maisch T, Bosl C, Szeimies RM, Lehn N, Abels C (2005) Photodynamic effects of Novel XF porphyrin derivatives on prokaryotic and eukaryotic cells. Antimicrob Agents Chemother 49:1542–1552.  https://doi.org/10.1128/AAC.49.4.1542-1552.2005 CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Malgikar S, Reddy SH, Sagar SV, Satyanarayana D, Reddy GV, Josephin JJ (2016) Clinical effects of photodynamic and low-level laser therapies as an adjunct to scaling and root planing of chronic periodontitis: a split-mouth randomized controlled clinical trial. Indian J Dent Res 27:121–126.  https://doi.org/10.4103/0970-9290.183130 CrossRefPubMedGoogle Scholar
  24. 24.
    Meisel P, Kocher T (2005) Photodynamic therapy for periodontal diseases: state of the art. J Photochem Photobiol B 79:159–170.  https://doi.org/10.1016/j.jphotobiol.2004.11.023 CrossRefPubMedGoogle Scholar
  25. 25.
    Melo WCMA (2014) Fotoinativação seletiva dos microrganismos: Escherichia coli e Staphylococcus aureus. Dissertation, Universidade de São PauloGoogle Scholar
  26. 26.
    Nagata JY, Hioka N, Kimura E, Batistela VR, Terada RS, Graciano AX et al (2012) Antibacterial photodynamic therapy for dental caries: evaluation of the photosensitizers used and light source properties. Photodiag Photodyn Ther 9:122–131.  https://doi.org/10.1016/j.pdpdt.2011.11.006 CrossRefGoogle Scholar
  27. 27.
    Odze RD, Marcial MA, Antonioli D (1996) Gastric fundic gland polyps: a morphological study including mucin histochemistry, stereometry, and MIB-1 immunohistochemistry. Hum Pathol 27:896–903.  https://doi.org/10.1016/S0046-8177(96)90215-4 CrossRefPubMedGoogle Scholar
  28. 28.
    Oliveira Sampaio SC, de C Monteiro JS, Cangussú MC, Pires Santos GM, dos Santos MA, dos Santos JN et al (2013) Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing. Lasers Med Sci 28:799–806.  https://doi.org/10.1007/s10103-012-1161-9 CrossRefPubMedGoogle Scholar
  29. 29.
    Park CH, Abramson ZR, Taba M, Jin Q, Chang J, Kreider JM, Goldstein AS, Giannobile WV (2007) Three-dimensional micro-computed tomographic imaging of alveolar bone in experimental bone loss or repair. J Periodontol 78:273–281.  https://doi.org/10.1902/jop.2007.060252 CrossRefPubMedPubMedCentralGoogle Scholar
  30. 30.
    Perussi JR (2007) Inativação fotodinâmica de microorganismos. Quim Nova 30:988–994.  https://doi.org/10.1590/S0100-40422007000400039 CrossRefGoogle Scholar
  31. 31.
    Pfitzner A, Sigush BH, Albrecht V, Glockmann E (2004) Killing of periodontopathogenic bacteria by photodynamic therapy. J Periodontol Res 75:1343–1349.  https://doi.org/10.1902/jop.2004.75.10.1343 CrossRefGoogle Scholar
  32. 32.
    Sigusch BW, Pfitzner A, Albrecht V, Glockmann E (2005) Efficacy of photodynamic therapy on inflammatory signs and two selected periodontopathogenic species in a beagle dog model. J Periodontol 76:1100–1105.  https://doi.org/10.1902/jop.2005.76.7.1100 CrossRefPubMedGoogle Scholar
  33. 33.
    Simplicio FI, Maionchi F, Hioka N (2002) Terapia fotodinâmica: aspectos farmacológicos, aplicações e avanços no desenvolvimento de medicamentos. Quim Nova 25:801–807.  https://doi.org/10.1590/S0100-40422002000500016 CrossRefGoogle Scholar
  34. 34.
    Stupáková V, Varinská L, Mirossay A, Sarisský M, Mojzis J, Dankovcík R et al (2009) Photodynamic effect of hypericin in primary cultures of human umbilical endothelial cells and glioma cell lines. Phytother Res 23:827–832.  https://doi.org/10.1002/ptr.2681 CrossRefPubMedGoogle Scholar
  35. 35.
    Umeda M, Takeuchi Y, Noguchi K, Huang Y, Koshy G, Ishikawa I (2004) Effects of nonsurgical periodontal therapy on the microbiota. Periodontol 2000 36:98–120.  https://doi.org/10.1111/j.1600-0757.2004.03675.x CrossRefPubMedGoogle Scholar
  36. 36.
    Vuong TT, Vever-Bizet C, Bonneau S, Bourg-Heckly G (2011) Hypericin incorporation and localization in fixed HeLa cells for various conditions of fixation and incubation. Photochem Photobiol Sci 10:561–568.  https://doi.org/10.1039/c0pp00324g CrossRefPubMedGoogle Scholar
  37. 37.
    Walsh LJ (1997) The current status of low level laser therapy in dentistry. Part 1. Soft tissue applications. Austr Dental J 42:247–254.  https://doi.org/10.1111/j.1834-7819.1997.tb00129.x CrossRefGoogle Scholar
  38. 38.
    Zanoli P (2004) Role of hyperforin in the pharmacological activities of St. John’s wort. CNS Drug Rev 10:203–218.  https://doi.org/10.1111/j.1527-3458.2004.tb00022.x CrossRefPubMedGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Paula Delello Macedo
    • 1
  • Sâmara Tfaile Corbi
    • 1
  • Fernanda Ali Kitagawa
    • 1
  • Janice Rodrigues Perussi
    • 2
  • Anderson Orzari Ribeiro
    • 3
  • Rosemary Adriana Chierici Marcantonio
    • 1
  1. 1.School of Dentistry Araraquara, Department of Diagnosis and SurgerySão Paulo State University (Unesp)AraraquaraBrazil
  2. 2.Chemistry and Molecular Physics DepartmentUSP, São Paulo UniversitySão CarlosBrazil
  3. 3.Centre for Natural Sciences and HumanitiesUFABC, Federal University of ABCSanto AndréBrazil

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