Bacterial antagonists and their cell-free cultures efficiently suppress canker disease in citrus lime

  • Natthida Sudyoung
  • Shinji Tokuyama
  • Sukhumaporn Krajangsang
  • Onanong Pringsulaka
  • Siriruk SarawaneeyarukEmail author
Original Article


Proliferation of citrus canker disease caused by Xanthomonas citri subsp. citri (Xcc) has increasingly become a serious threat and has resulted in a significant loss in citrus production worldwide. This research aimed to identify efficient antagonistic bacteria for the biological control of canker disease in Citrus aurantifolia cultivar Pan (Pan-lime) and their antagonistic characteristics by experimental analysis. With the dual culture method, twenty isolates inhibited Xcc. Eight out of over fifty isolates showed clear inhibition zones and were further analyzed for plant growth-promoting characteristics. After the screening process, Bacillus velezensis isolate SWUA08 and Pseudomonas aeruginosa isolate SWUC02 were selected for further analysis of bacterial canker resistance in Pan-lime seedlings and trees. Experimental results show that both antagonists increased canker disease resistance. Furthermore, their cell-free cultures reduced canker disease severity index in Pan-lime tree. Our experimental results demonstrating that the antagonists offer an alternative perspective to evaluate a method of canker disease inhibition.


Canker disease Antagonist Xanthomonad Biocontrol Citrus 



This work was supported by Srinakharinwirot University [Grant Numbers 762/2558].

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

41348_2019_295_MOESM1_ESM.pdf (14 kb)
Supplementary material 1 (PDF 13 kb)
41348_2019_295_MOESM2_ESM.pdf (101 kb)
Supplementary material 2 (PDF 101 kb)


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Copyright information

© Deutsche Phytomedizinische Gesellschaft 2019

Authors and Affiliations

  • Natthida Sudyoung
    • 1
  • Shinji Tokuyama
    • 2
  • Sukhumaporn Krajangsang
    • 3
  • Onanong Pringsulaka
    • 3
  • Siriruk Sarawaneeyaruk
    • 3
    Email author
  1. 1.Department of Biology, Faculty of ScienceSrinakharinwirot UniversityWatthanaThailand
  2. 2.Department of Applied Biological Chemistry, Graduate School of AgricultureShizuoka UniversityShizuokaJapan
  3. 3.Department of Microbiology, Faculty of ScienceSrinakharinwirot UniversityWatthanaThailand

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