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Lethal and parasitism effects of selected novel pesticides on adult Trichogramma chilonis (Hymenoptera: Trichogrammatidae)


Integration of chemical and biological controls is an important tactic of integrated pest management. Trichogramma chilonis (Ishii) is an important natural enemy of lepidopteran pests. However, the minute parasitoids are adversely affected by most pesticides. Effects of acute mortality and parasitism of selected novel pesticides on adults of T. chilonis exposed to the dried residues of field doses for 24 h on glass surface were evaluated. Acetamiprid, spinetoram and fipronil induced mortality of > 95%, followed by abamectin (ranged 71.3–83.5%) in four residual-age treatments. Spiromesifen, haloxyfop-p-methyl, mixture of pyraclostrobin + metiram and mixture of trifloxystrobin + tebuconazole revealed mortality of ≤ 36.4% in all treatments (when female parasitoids were exposed to 1 day, 5 days, 10 days and 15 days after treatments). Bispyribac sodium induced mortality of ranged 51.1–54.9% in 1- and 5-day treatments, and approximately ≤ 10% in others (10-day and 15-day treatment). Eggs of Sitotroga cerealella in acetamiprid, spinetoram, fipronil and abamectin treatments showed ≤ 36% parasitism relative to controls in all treatments. Spirotetramat, bispyribac sodium, nicosulfuron, trifloxystrobin + tebuconazole and pyraclostrobin + metiram resulted in parasitism rates of 49.2–71.2% and 81.5–93.8% in 1-day and 15-day treatment, respectively. Helicoverpa armigera nucleopolyhedrovirus, chlorantraniprole, spiromesifen haloxyfop-p-methyl, chlorothalonil + procymidone and myclobutanil showed ≥ 80.6% parasitism in all treatments. Thus, acetamiprid, spinetoram, fipronil and abamectin were the most harmful among the pesticides used for both emergence and parasitism, while remaining pesticides demonstrated relatively compatibility with the emergence as well as parasitism by the minute parasitoids, and can be integrated with the minute parasitoid in agroecosystem.

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I am very much grateful to the Higher Education Commission of Pakistan for the financial support to my research. I also wish to acknowledge the Nuclear Institute of Food and Agriculture (NIFA), Tarnab, Peshawar, for the provision of laboratory. I am also thankful to Dr. John R Ruberson, Head, Department of Entomology, University of Nebraska, Lincoln, USA, for revised and corrected the English language used in this manuscript. I am also very grateful to Jos Feys, senior research fellow and faculty member of movement and rehabilitation sciences at the KU Leuven university (Catholic University of Leuven, Belgium) for statistical analysis of the data.


This study was funded by Higher Education Commission of Pakistan under indigenous 5000 PhD fellowship program batch IV with research Grant Number 074-3591-Bm4-011.

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Correspondence to Muhammad Ashraf Khan.

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Khan, M.A. Lethal and parasitism effects of selected novel pesticides on adult Trichogramma chilonis (Hymenoptera: Trichogrammatidae). J Plant Dis Prot 127, 81–90 (2020). https://doi.org/10.1007/s41348-019-00280-2

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  • Novel pesticides
  • Trichogramma chilonis
  • Residual treatment
  • Mortality
  • Parasitism