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Monitoring activities on fungicide resistance in Botrytis cinerea carried out in vineyards in North-West Italy in 2018

  • Domenico BertettiEmail author
  • Matteo Monchiero
  • Angelo Garibaldi
  • Maria Lodovica Gullino
Short Communication
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Abstract

A monitoring was conducted in 35 commercial vineyards located in Piedmont (North-West Italy) in September 2018 to test the sensitivity of Botrytis cinerea populations to the fungicides that were commonly used in the past and at present: benzimidazoles, dicarboximides, anilinopyrimidines, phenylpyrroles, hydroxyanilides and SDHI fungicides. Sensitivity was tested by evaluating the spore germination on a medium amended with a discriminatory concentration of the different tested botryticides. Conidial suspensions were prepared from infected, randomly sampled bunches and used for the tests. In comparison with the years 2008–2014, the following trend was recorded: benzimidazole resistance was quite stable and was found in about 50% of the vineyards involved in the monitoring; dicarboximide resistance had disappeared in the tested samples, while the frequency of resistance to anilinopyrimidines had increased; the resistance to fenhexamid had decreased; in the case of boscalid, the presence of resistance was quite stable; no resistance to phenylpyrroles was found, although the high discriminatory dose used for fludioxonil cannot exclude the presence in the tested vineyards of low levels of resistance to this fungicide, typical of multidrug-resistant strains.

Keywords

Botryticides Grapevine Grey mould Monitoring 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This research did not involve human participants or any animal experimentation.

References

  1. Avenot H, Quattrini J, Puckett R, Michailides TJ (2018) Different levels of resistance to cyprodinil and iprodione and lack of fludioxonil resistance in Botrytis cinerea isolates collected from pistachio, grape, and pomegranate fields in California. Crop Prot 112:274–281CrossRefGoogle Scholar
  2. Baggio JS, Peres NA, Amorim L (2018) Sensitivity of Botrytis cinerea isolates from conventional and organic strawberry fields in Brazil to azoxystrobin, iprodione, pyrimethanil, and thiophanate-methyl. Plant Dis 102:1803–1809CrossRefGoogle Scholar
  3. Banno S, Fukumori F, Ichiishi A, Okada K, Uekusa H, Kimura M, Fujimura M (2008) Genotyping of benzimidazole-resistant and dicarboximide-resistant mutations in Botrytis cinerea using real-time polymerase chain reaction assays. Phytopathology 98:97–404CrossRefGoogle Scholar
  4. Bertetti D, Monchiero M, Garibaldi A, Gullino ML (2016) Evoluzione della resistenza ai fungicidi di popolazioni di Botrytis cinerea nei vigneti dell’Italia nord-occidentale. Protezione delle Colture 9(3):12–17Google Scholar
  5. Billard A, Fillinger S, Leroux P, Lachaise H, Beffa R, Debieu D (2012) Strong resistance to the fungicide fenhexamid entails a fitness cost in Botrytis cinerea, as shown by comparisons of isogenic strains. Pest Manag Sci 68:684–691CrossRefGoogle Scholar
  6. Campia P, Venturini G, Moreno-Sanz P, Casati P, Toffolatti SL (2017) Genetic structure and fungicide sensitivity of Botrytis cinerea populations isolated from grapevine in northern Italy. Plant Pathol 66:890–899CrossRefGoogle Scholar
  7. De Guido MA, De Miccolis Angelini RM, Pollastro S, Santomauro A, Faretra F (2010) Selection and genetic analysis of laboratory mutants of Botryotinia fuckeliana resistant to fenhexamid. J Plant Pathol 89:203–210Google Scholar
  8. De Miccolis Angelini RM, Masiello M, Rotolo C, Pollastro S, Faretra F (2014a) Molecular characterisation and detection of resistance to succinate dehydrogenase inhibitor fungicides in Botryotinia fuckeliana (Botrytis cinerea). Pest Manag Sci 70:1884–1893CrossRefGoogle Scholar
  9. De Miccolis Angelini RM, Rotolo C, Masiello M, Gerin D, Pollastro S, Faretra F (2014b) Occurrence of fungicide resistance in populations of Botryotinia fuckeliana (Botrytis cinerea) on table grape and strawberry in southern Italy. Pest Manag Sci 70:1785–1796CrossRefGoogle Scholar
  10. Fernández-Ortuño D, Fengping C, Schnabel G (2013) Resistance to cyprodinil and lack of fludioxonil resistance in Botrytis cinerea isolates from strawberry in North and South Carolina. Plant Dis 97:81–85CrossRefGoogle Scholar
  11. George AB, Thomas V, Olga K, George SK (2010) Multiple resistance of Botrytis cinerea from kiwifruit to SDHIs, QoIs and fungicides of other chemical groups. Pest Manag Sci 66:967–973CrossRefGoogle Scholar
  12. Grabke A, Stammler G (2015) A Botrytis cinerea population from a single strawberry field in Germany has a complex fungicide resistance pattern. Plant Dis 99:1078–1086CrossRefGoogle Scholar
  13. Gullino ML (1992) Chemical control of Botrytis spp. In: Verhoeff K, Malathrakis NE, Williamson B (eds) Recent advances in Botrytis research. Pudoc Scientific Publishers, Wageningen, pp 217–222Google Scholar
  14. Gullino ML, Garibaldi A (1986) Fungicide resistance monitoring as an aid to tomato gray mold management. Proc Br Crop Prot Conf 1:277–505Google Scholar
  15. Gullino ML, Bertetti D, Monchiero M, Garibaldi A (2000) Sensitivity to anilinopyrimidines and phenylpyrroles in Botrytis cinerea in north-Italian vineyards. Phytopathol Mediter 39:433–446Google Scholar
  16. Gullino ML, Bertetti D, Garibaldi A (2012) Fungicide resistance in Italian agriculture and strategies for its management. In: Thind TS (ed) Fungicide resistance in crop protection: risk and management. CAB International, Wallingford, pp 184–190CrossRefGoogle Scholar
  17. Kretschmer M, Leroch M, Mosbach A, Walker AS, Fillinger S, Mernke D, Schoonbeek HJ (2009) Fungicide-driven evolution and molecular basis of multidrug resistance in field populations of the gray mold fungus Botrytis cinerea. PLoS Pathog.  https://doi.org/10.1371/journal.ppat.1000696 Google Scholar
  18. La Torre BA, Torres R (2012) Prevalence of isolates of Botrytis cinerea resistant to multiple fungicides in Chilean vineyards. Crop Prot 40:49–52CrossRefGoogle Scholar
  19. Leroch M, Kretschmer M, Hahn M (2011) Fungicide resistance phenotypes of Botrytis cinerea isolates from commercial vineyards in south west Germany. J Phytopathol 159:63–65CrossRefGoogle Scholar
  20. Leroux P (2004) Chemical control of Botrytis and its resistance to chemical fungicides. In: Elad Y, Williamson B, Tudzynski P, Delen N (eds) Botrytis: biology, pathology and control. Kluwer Academic Publishers, Dordrecht, pp 195–222Google Scholar
  21. Leroux P, Clerjeau M (1985) Resistance of Botrytis cinerea Pers. and Plasmopara viticola (Berl. and de Toni) to fungicides in French vineyards. Crop Prot 4(2):137–160CrossRefGoogle Scholar
  22. Leroux P, Gredt M (1995) Ėtude in vitro de la résistance de Botrytis cinerea aux fungicides anilinopyrimidines. Agronomie 15:367–370CrossRefGoogle Scholar
  23. Leroux P, Walker AS (2013) Activity of fungicides and modulators of membrane drug transporters in field strains of Botrytis cinerea displaying multidrug resistance. Eur J Plant Pathol 135:683–693CrossRefGoogle Scholar
  24. Li X, Fernades-Ortuño D, Grabke A, Schnabel G (2014) Resistance to fludioxonil in Botrytis cinerea isolates from strawberry and blackberry. Phytopathology 104:724–732CrossRefGoogle Scholar
  25. Oliveira MS, Amiri A, Zuniga AI, Peres NA (2017) Sources of primary inoculum of Botrytis cinerea and their impact on fungicide resistance development in commercial strawberry fields. Plant Dis 101:1761–1768CrossRefGoogle Scholar
  26. Saito S, Cadle-Davidson L, Wilcox WF (2014) Selection, fitness and control of grape isolates of Botrytis cinerea variably sensitive to Fenhexamid. Plant Dis 98:233–240CrossRefGoogle Scholar
  27. Stammler G, Speakman J (2006) Microtiter method to test the sensitivity of Botrytis cinerea to boscalid. J Phytopathol 154:508–510CrossRefGoogle Scholar
  28. Vercesi A, Toffolatti SL, Venturini G, Campia P, Scagnelli S (2014) Characterization of Botrytis cinerea populations associated with treated and untreated cv. Moscato vineyards. Phytopathol Mediter 53(1):108–123Google Scholar
  29. Walker AS, Micoud A, Gautier A, Confais J, Martinho D, Viaud M, Le Pêcheur P, Dupont J, Fournier E (2011) Botrytis pseudocinerea, a new cryptic species causing gray mold in French vineyards in sympatry with Botrytis cinerea. Phytopathology 101:1433–1445CrossRefGoogle Scholar
  30. Walker AS, Micoud A, Florent Rémuson F, Grosman J, Gredta M, Leroux P (2013) French vineyards provide information that opens ways for effective resistance management of Botrytis cinerea (gray mold). Pest Manag Sci 69:667–678CrossRefGoogle Scholar
  31. Weber RWS, Entrop A-P (2017) Recovery of Botrytis strains with multiple fungicide resistance from raspberry nursery plants. Eur J Plant Pathol 147:933–936CrossRefGoogle Scholar
  32. Yarden O, Katan T (1993) Mutations leading to substitutions at amino acids 198 and 200 of beta-tubulin that correlate with benomyl-resistance phenotypes of field strains of Botrytis cinerea. Phytopathology 83:1478–1483CrossRefGoogle Scholar

Copyright information

© Deutsche Phytomedizinische Gesellschaft 2019

Authors and Affiliations

  • Domenico Bertetti
    • 1
    Email author
  • Matteo Monchiero
    • 1
  • Angelo Garibaldi
    • 1
  • Maria Lodovica Gullino
    • 1
    • 2
  1. 1.Center of Competence for the Innovation in the Agro-environmental Sector (AGROINNOVA)University of TorinoGrugliascoItaly
  2. 2.Dipartimento di Scienze Agrarie, Forestali e Alimentari (DI.S.A.F.A.)University of TorinoGrugliascoItaly

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