Analysis of genetic diversity among Macrophomina phaseolina (Tassi) Goid. isolates from Euro-Asian countries

  • Sonja Tančić ŽivanovEmail author
  • Boško Dedić
  • Aleksandra Dimitrijević
  • Nenad Dušanić
  • Siniša Jocić
  • Vladimir Miklič
  • Branislav Kovačević
  • Dragana Miladinović
Original Article


The study assessed the genetic variability among Macrophomina phaseolina (Tassi) Goid. isolates originating from six different countries (Turkey, Bulgaria, Romania, Spain, Ukraine and Serbia), collected mostly from sunflower, but also maize, soybean, flax, common bean and zebra plant. RAPD analysis was based on 154 polymorphic bands obtained with 14 OPA primers. RAPD markers OPA-02–OPA-05, OPA-07–OPA-13 and OPA-18–OPA-20 were found to be suitable for measuring the genetic relatedness and detecting variability in M. phaseolina populations. Cluster analysis revealed high genetic diversity of M. phaseolina isolates within the same country of origin, but clear differentiation according to the isolates’ host plant and geographical origin was not observed. Contingency analysis and corrected contingency coefficients enabled determination of RAPD markers associated with geographical origin of isolates, as well as markers specific for non-sunflower isolates. Marker OPA 10_1750 was found to be highly specific for the isolates from Turkey, while two markers, OPA 3_1250 and OPA 9_800, could be used for differentiation of isolates originating from sunflower from isolates originating from other host plants.


Charcoal rot Genetic variability Sunflower Plant host Geographical origin 



This research study is part of the project TR 31025 supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia. The authors are especially thankful to Dr. Valentina Entcheva (Dobrudzha Agricultural Institute, Dobrudzha, Bulgaria) for providing the fungal isolates from Bulgaria, as well as Dr. Yalcin Kaya (Plant Breeding Research Center and Genetic Engineering Department, Trakya University, Edirne, Turkey), Dr. Goksal Evci (Trakya Agricultural Research Institute, Edirne, Turkey) and Dr. Gülden Hazarhun (Pia Frucht, Bursa, Turkey) for providing the fungal isolates from Turkey.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Supplementary material

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Supplementary material 1 (DOCX 12 kb)
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Supplementary material 2 (TIFF 646 kb)
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Supplementary material 3 (TIFF 1011 kb)


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Copyright information

© Deutsche Phytomedizinische Gesellschaft 2019

Authors and Affiliations

  • Sonja Tančić Živanov
    • 1
    Email author
  • Boško Dedić
    • 1
  • Aleksandra Dimitrijević
    • 1
  • Nenad Dušanić
    • 1
  • Siniša Jocić
    • 1
  • Vladimir Miklič
    • 1
  • Branislav Kovačević
    • 2
  • Dragana Miladinović
    • 1
  1. 1.Institute of Field and Vegetable CropsNovi SadSerbia
  2. 2.Institute of Lowland Forestry and EnvironmentUniversity of Novi SadNovi SadSerbia

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