Journal of Plant Diseases and Protection

, Volume 126, Issue 6, pp 575–584 | Cite as

AFLP as a fingerprinting tool for characterising isolates of Fusarium oxysporum f. sp. elaeidis causal organism for fusarium wilt disease of oil palm in Ghana

  • Kwasi Adusei-FosuEmail author
  • Matthew Dickinson
  • Egya Ndede Yankey
Original Article


Fusarium wilt is a damaging disease of oil palm in Ghana. Genetic diversity of Fusarium oxysporum f. sp. elaeidis (Foe) the causal organism of the disease was studied via AFLP technique using fluorescence-based automated system for Foe isolates from Ghana in comparison with other Foe isolates from different geographical locations. AFLP was conducted using thirteen MseI primers with three sets of EcoRI primers to generate individual phylogenetic trees. A concatenated phylogenetic tree was constructed from AFLP data using the three primer pairs. Individual phylogenetic trees for each of the primers could cluster Foe isolates from Ghana distinctively from Foe isolates collected in different geographical locations. Isolates from Ghana could not be clustered under one clade for all the individual phylogenetic trees, and this was further confirmed in the concatenated phylogenetic trees for the three-primer dataset that involved 4438 and 117 random markers of polymorphic bands. The use of AFLP has shown more genetic information about Foe isolates from Ghana compared with other collections from different geographical areas. AFLP markers showed all Foe isolates from Ghana are not monophyletic.


Fusarium wilt disease Fusarium oxysporum f. sp. elaeidis Phylogenetic AFLP 



This research was fully funded by Commonwealth Scholarship Commission, UK. We thank Dr. Julie Flood the Global Director of CABI Research for technical advice and support and the late Prof. Richard Cooper former Lecturer at University of Bath, UK, for providing us with two isolates of Foe. Our appreciation also goes to University of Nottingham, UK, Plant Science School of Biosciences for giving us access to laboratory facilities. We finally thank all the various oil palm plantation sites in Ghana where sampling was undertaken including CSIR-OPRI, NORPALM, BOPP and TOPP.


This study was funded by Commonwealth Scholarship Commission, UK, with grant number (GHCS115: 2013).

Compliance with ethical standards

Conflict of interest

Author Dr. Kwasi Adusei-Fosu currently a Forest Pathologist with Scion (New Zealand Forest Research Institute), received research grant from Commonwealth Scholarship Commission, UK, and was supervised by Co-author Prof. Matthew Dickinson, Senior Plant Pathologist, Diagnostician Researcher, and Lecturer at the University of Nottingham, UK. Also, there is no conflict of interest in existence: Author Dr. Kwasi Adusei-Fosu declares that he has no conflict of interest. Author Prof. Matthew Dickinson and Dr. Ndede Yankey also declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by authors.


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Copyright information

© Deutsche Phytomedizinische Gesellschaft 2019

Authors and Affiliations

  1. 1.Scion, Forest ProtectionRotoruaNew Zealand
  2. 2.Division of Plant and Crop SciencesUniversity of NottinghamSutton BoningtonUK
  3. 3.Oil Palm Research InstituteKadeGhana

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