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Journal of Plant Diseases and Protection

, Volume 126, Issue 6, pp 575–584 | Cite as

AFLP as a fingerprinting tool for characterising isolates of Fusarium oxysporum f. sp. elaeidis causal organism for fusarium wilt disease of oil palm in Ghana

  • Kwasi Adusei-FosuEmail author
  • Matthew Dickinson
  • Egya Ndede Yankey
Original Article

Abstract

Fusarium wilt is a damaging disease of oil palm in Ghana. Genetic diversity of Fusarium oxysporum f. sp. elaeidis (Foe) the causal organism of the disease was studied via AFLP technique using fluorescence-based automated system for Foe isolates from Ghana in comparison with other Foe isolates from different geographical locations. AFLP was conducted using thirteen MseI primers with three sets of EcoRI primers to generate individual phylogenetic trees. A concatenated phylogenetic tree was constructed from AFLP data using the three primer pairs. Individual phylogenetic trees for each of the primers could cluster Foe isolates from Ghana distinctively from Foe isolates collected in different geographical locations. Isolates from Ghana could not be clustered under one clade for all the individual phylogenetic trees, and this was further confirmed in the concatenated phylogenetic trees for the three-primer dataset that involved 4438 and 117 random markers of polymorphic bands. The use of AFLP has shown more genetic information about Foe isolates from Ghana compared with other collections from different geographical areas. AFLP markers showed all Foe isolates from Ghana are not monophyletic.

Keywords

Fusarium wilt disease Fusarium oxysporum f. sp. elaeidis Phylogenetic AFLP 

Notes

Acknowledgement

This research was fully funded by Commonwealth Scholarship Commission, UK. We thank Dr. Julie Flood the Global Director of CABI Research for technical advice and support and the late Prof. Richard Cooper former Lecturer at University of Bath, UK, for providing us with two isolates of Foe. Our appreciation also goes to University of Nottingham, UK, Plant Science School of Biosciences for giving us access to laboratory facilities. We finally thank all the various oil palm plantation sites in Ghana where sampling was undertaken including CSIR-OPRI, NORPALM, BOPP and TOPP.

Funding

This study was funded by Commonwealth Scholarship Commission, UK, with grant number (GHCS115: 2013).

Compliance with ethical standards

Conflict of interest

Author Dr. Kwasi Adusei-Fosu currently a Forest Pathologist with Scion (New Zealand Forest Research Institute), received research grant from Commonwealth Scholarship Commission, UK, and was supervised by Co-author Prof. Matthew Dickinson, Senior Plant Pathologist, Diagnostician Researcher, and Lecturer at the University of Nottingham, UK. Also, there is no conflict of interest in existence: Author Dr. Kwasi Adusei-Fosu declares that he has no conflict of interest. Author Prof. Matthew Dickinson and Dr. Ndede Yankey also declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by authors.

References

  1. Abd-Elsalam KA, Aly IN, Mohmed A, Khalil SM, Verreet JA (2003) PCR identification of Fusarium genus based on nuclear ribosomal-DNA sequence data. Afr J Biotechnol 2:82–85Google Scholar
  2. Abd-Elsalam KA, Omar MR, Migheli Q, Nirenberg HI (2004) Genetic characterization of Fusarium oxysporum f. sp. vasinfectum isolates by random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). J Plant Dis Prot 111:534–544Google Scholar
  3. Aderungboye FO (1982) Significance of vascular wilt in oil palm plantation in Nigeria. In: Pushparajah E, Chew PS (eds) The oil palm and agriculture in the eighties, vol 2. Incorporated Society of Planters, Kuala Lumpur, pp 475–484Google Scholar
  4. Angelucci F (2013) Analysis of incentives and disincentives for palm oil in Ghana FAO, RomeGoogle Scholar
  5. Baayen RP, O’Donnell K, Bonants PJM, Cigelnik E, Kroon LPNM, Roebroeck EJA, Waalwijk C (2000) Gene genealogies and AFLP analyses within the Fusarium oxysporum complex identify monophyletic and non-monophyletic Formae specialis causing wilt and rot disease. Phytopathology 90:891–900PubMedGoogle Scholar
  6. Belabid L, Baum M, Fortas Z, Bouznad Z, Eujayl I (2004) Pathogenic and genetic characterisation of Algerian isolates of Fusarium oxysporum f. sp. lentis by RAPD and AFLP analysis. Afr J Biotech 3:25–31Google Scholar
  7. Cooper RM (2011) Fusarium wilt of oil palm: a continuing threat to South East Asian plantations. The Planter 87:409–418Google Scholar
  8. Corley RHV, Tinker PB (2003) The oil palm, 4th edn. Blackwell, Oxford, pp 133–198Google Scholar
  9. Crawford AR, Bassam BJ, Drenth A, MacLean DJ, Irwin JAG (1996) Evolutionary relationships among Phytophthora species deduced from rDNA sequence analysis. Mycol Res 100:437–443Google Scholar
  10. De Franqueville H, Diabate S (2004) Status on the oil palm vascular wilt. In: MPOB proceedings of the international conference on pests and diseases of importance to the oil palm industry, Kuala LumpurGoogle Scholar
  11. De Franqueville H, Diabaté S (1995) Oil palm vascular wilt in West Africa. PRD 2:5–13Google Scholar
  12. De Franqueville H, Renard JL (1990) Improvement of oil palm vascular wilt tolerance. Results and development of the disease at the R. Michaux plantation. Oléagineux 45:399–405Google Scholar
  13. Dossa C, Pandobahuon A, Renard JL, Boisson C (1991) Determination of vegetative compatibility groups in African Fusarium oxysporum strains isolated from vascular wilt-infected oil palms. Oleagineux 46:145–147Google Scholar
  14. Elliott ML, Des Jardin EA, O’Donnell K, Geiser DM, Harrison NA, Broschat TK (2010) Fusarium oxysporum f. sp. palmarum, a Novel Forma Specialis causing a lethal disease of Syagrus romanzoffiana and Washingtonia robusta in Florida. Plant Dis 94:31–38PubMedGoogle Scholar
  15. FAO (2014) FAOSTAT databaseGoogle Scholar
  16. Flood J, Mepsted R, Cooper RM (1990) Contamination of oil palm pollen and seeds by Fusarium spp. Mycol Res 94:708–709Google Scholar
  17. Fourie G, Steenkamp ET, Gordon TR, Viljoen A (2009) Evolutionary relationships among the Fusarium oxysporum f. sp. cubense vegetative compatibility groups. Appl Environ Microbiol 75:4770–4781PubMedPubMedCentralGoogle Scholar
  18. Fraselle JV (1951) Experimental evidence of the pathogenicity of Fusarium oxysporum Schl. f. to the oil palm (Elaeis guineensis J.). Nature 167:447PubMedGoogle Scholar
  19. Gordon TR, Martyn RD (1997) The evolutionary biology of Fusarium oxysporum. Annu Rev Phytopathol 35:111–128PubMedGoogle Scholar
  20. Guldentops RE (1962) Contribution à l’étude de la trachéomycose du palmier à huile. Parasitica 18:244–263Google Scholar
  21. Gurjar G, Barve M, Giri A, Gupta V (2009) Identification of Indian pathogenic races of Fusarium oxysporum f. sp. ciceris with gene specific, ITS and random markers. Mycologia 101:480–491Google Scholar
  22. Hampl V, Pavlícek A, Flegr J (2001) Construction and bootstrap analysis of DNA fingerprinting-based phylogenetic trees with the freeware program FreeTree: application to trichomonad parasites. Int J Syst Evolut Microbiol 51:731–735Google Scholar
  23. Ho YW, Varghese G (1986) Pathogenic potential of soil Fusaria from Malaysian oil palm habitats. J Phytopathol 115:325–328Google Scholar
  24. Ho YW, Varghese G, Taylor GS (1985) Pathogenicity of Fusarium oxysporum isolates from Malaysia and F. oxysporum f. sp. elaedis from Africa to seedlings of oil palm (Elaeis guineensis). J Phytopathol 114:193–202Google Scholar
  25. Jonkers W, Rodrigues CDA, Rep M (2009) Impaired colonization and infection of tomato roots by the Δfrp1 mutant of Fusarium oxysporum correlates with reduced CWDE gene expression. Society 22:507–518Google Scholar
  26. Kawabe M, Kobayashi Y, Okada G, Yamaguchi I, Teraoka T, Arie T (2005) Three evolutionary lineages of tomato wilt pathogen, Fusarium oxysporum f. sp. lycopersici, based on sequences of IGS, MAT1, and pg1, are each composed of isolates of a single mating type and a single or closely related vegetative compatibility group. J Gen Plant Pathol 71:263–272Google Scholar
  27. Kovachich WG (1948) A preliminary anatomical note on vascular wilt disease of the oil palm (Elaeis guineensis). Ann Bot 12:327–329Google Scholar
  28. Laurence MH, Summerell BA, Liew ECY (2015) Fusarium oxysporum f. sp. canariensis: evidence for horizontal gene transfer of putative pathogenicity genes. Plant Pathol 64:1068–1075Google Scholar
  29. Locke T, Colhoun J (1973) Fusarium oxysporum f. sp elaeidis as a seed borne pathogen. Trans Br Mycol Soc 60:594–595Google Scholar
  30. Ma L-J, van der Does HC, Borkovich KA, Coleman JJ, Daboussi M-J et al (2010) Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium. Nature 464:367–373PubMedPubMedCentralGoogle Scholar
  31. Mepsted R, Nyandusa C, Flood J, Cooper RM (1991) A non-destructive, quantitative method for the assessment of infection of oil palm by Fusarium oxysporum f.sp. elaeidis. Int J Oil Palm Res 3:329–335Google Scholar
  32. Mohmed A, Abdel-Satar M, Khalil S, Mohmed IN, Abd-Elsalam KA, Verreet JA (2003) Molecular phylogeny of Fusarium species by AFLP fingerprint. Afr J Biotech 2:51–55Google Scholar
  33. Mouyna I, Renard JL, Brygoo Y (1996) DNA polymorphism among Fusarium oxysporum f. sp. elaeidis populations from oil palm, using a repeated and dispersed sequence “Palm”. Curr Genet 30:174–180PubMedGoogle Scholar
  34. Nei M, Li W-H (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci 76:5269–5273PubMedGoogle Scholar
  35. O’Donnell K, Kistler HC, Cigelnik E, Ploetz RC (1998) Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci USA 1995:2044–2049Google Scholar
  36. Ofosu-Budu K, Sarpong D (2013) Oil palm industry growth in Africa: a value chain and smallholders study for Ghana In: Elbehri A(ed) Rebuilding West Africa’s food potential. FAO/IFAD, pp 349–38Google Scholar
  37. Papavizas G (1967) Evaluation of various media and antimicrobial agents for isolation of Fusarium from soil. Phytophathology 57:848–852Google Scholar
  38. Pavlicek A, Hrda S, Flegr J (1999) Short communication freetree-freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia. Folia Biol (Praba) 45:97–99Google Scholar
  39. Prendergast AG (1957) Observations on the epidemiology of vascular wilt disease of the oil palm. J West Afr Inst Oil Palm Res 2:148–175Google Scholar
  40. Renard JL, De Franqueville H (1989) Oil palm vascular wilt. Oleagineux 44:342–347Google Scholar
  41. Renard JL, Quillec G (1983) Fusariose et replantation de palmiers à huile en zone fusariée en Afrique de l’ouest (trilingue fr. –angl. –esp). Conseil de l’IRHO No 235. Oléagineux 387:421–427Google Scholar
  42. Rhebergen T, Fairhurst T, Zingore S, Fisher M, Oberthür T, Whitbread A (2016) Climate, soil and land-use based land suitability evaluation for oil palm production in Ghana. Eur J Agron 81:1–14Google Scholar
  43. Rusli MH, Idris AS, Cooper RM (2015) Evaluation of Malaysian oil palm progenies for susceptibility, resistance or tolerance to Fusarium oxysporum f. sp. elaeidis and defence-related gene expression in roots. Plant Pathol 64:638–647Google Scholar
  44. Rusli HM, Wheals AE, Sharma S, Seman I, Cooper R (2017) Disease epidemiology and genetic diversity of Fusarium oxysporum f. sp. elaeidis, cause of fusarium wilt of oil palm (Elaeis guineensis Jacq.). J Oil Palm Res 29:1–14Google Scholar
  45. Sanders IR (2002) Ecology and evolution of multigenomic arbuscular mycorrhizal fungi. Am Nat 160:8–41Google Scholar
  46. Schardl CL, Craven KD (2003) Interspecific hybridization in plant-associated fungi and oomycetes: a review. Mol Ecol 12:2861–2873PubMedGoogle Scholar
  47. Silva FP, Marta HV, Ricardo H (2014) EF-1α gene and IGS rDNA sequencing of Fusarium oxysporum f. sp. vasinfectum and F. oxysporum f. sp. phaseoli reveals polyphyletic origin of strains. Trop Plant Pathol 39:64–73Google Scholar
  48. Turner PD (1981) Oil palm diseases and disorders. Oxford University Press, OxfordGoogle Scholar
  49. Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M et al (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414PubMedPubMedCentralGoogle Scholar
  50. Wang B, Brubaker CL, Tate W, Woods MJ, Matheson BA, Burdon JJ (2006) Genetic variation and population structure of Fusarium oxysporum f. sp. vasinfectum in Australia. Plant Pathol 55:746–755Google Scholar
  51. Wardlaw CW (1946) Fusarium oxysporum on the oil palm. Nature 158:712Google Scholar
  52. Wardlaw CW (1950) Vascular wilt disease of the oil palm caused by Fusarium oxysporum Schl. Trop Agric 1:24–59Google Scholar
  53. Waterston JM (1953) Observations on the influence of some ecological factors on the incidence of oil palm diseases in Nigeria. J West Afr Inst Oil Palm Res 1:24–59Google Scholar
  54. White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press Inc, New York, pp 315–322Google Scholar

Copyright information

© Deutsche Phytomedizinische Gesellschaft 2019

Authors and Affiliations

  1. 1.Scion, Forest ProtectionRotoruaNew Zealand
  2. 2.Division of Plant and Crop SciencesUniversity of NottinghamSutton BoningtonUK
  3. 3.Oil Palm Research InstituteKadeGhana

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