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Journal of Plant Diseases and Protection

, Volume 125, Issue 5, pp 469–476 | Cite as

Incidence, distribution and limited genetic variability among Turkish isolates of Grapevine Pinot gris virus from different grapevine cultivars

  • Eminur Elçi
  • Mona Gazel
  • Vahid Roumi
  • Kadriye Çağlayan
Original Article
  • 31 Downloads

Abstract

Grapevine Pinot gris virus (GPGV) was firstly identified in northern Italy by deep sequencing from grapevine cv. Pinot gris, exhibiting mottling and deformation of the leaves. The objective of this study was to investigate the prevalence and genetic variability of GPGV isolates obtained from different local and imported grapevine cultivars in Turkey based on partial coat protein, movement protein and RNA-dependent RNA polymerase (RdRp) domain of the replicase (Rep) gene. Two hundred and one grapevine samples from different provinces were tested by RT-PCR assays, approximately 25% of which were found to be infected by GPGV. The PCR products were sequenced and based on the phylogenetic analysis, RdRp gene was found to be most conserved region. The phylograms of three genomic regions revealed correlation between geography and genetic structure. Furthermore, nucleotide diversity studies revealed a low divergence from the homologous sequences from GenBank and some variations within the groups were detected. The results presented in this study provide a better understanding of genetic variation and phylogenetic of GPGV isolates worldwide.

Keywords

Vitis vinifera L. GPGV CP MP Rep Genetic variation 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest in the research.

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Copyright information

© Deutsche Phytomedizinische Gesellschaft 2018

Authors and Affiliations

  • Eminur Elçi
    • 1
  • Mona Gazel
    • 2
  • Vahid Roumi
    • 3
  • Kadriye Çağlayan
    • 2
  1. 1.Plant Production and Technologies Department, Faculty of Agricultural Sciences and TechnologiesNiğde Ömer Halisdemir UniversityNiğdeTurkey
  2. 2.Plant Protection Department, Agriculture FacultyMustafa Kemal UniversityHatayTurkey
  3. 3.Plant Protection Department, Faculty of AgricultureUniversity of MaraghehMaraghehIran

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