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APPJ Discharge Effects on Human Factor VIII:Ag Level in Healthy and Hemophilia A Patient

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Many studies in the field of cold plasma and its application in medicine and biology have been done recently. Some of them reported that cold atmospheric pressure plasma jet (APPJ) discharge accelerates blood clotting because factor VIII (FVIII) is a plasma glycoprotein that may play a role in the coagulation cascade. In this study, we aim to assess the effect of APPJ discharge on factor VIII antigen (FVIII:Ag) in healthy and hemophilic patients. Therefore, the variations of FVIII:Ag in healthy plasma samples and hemophilic plasma samples at the times of 0, 4, 8 and 16 s treatment with APPJ were measured by ELISA technique. Additionally, activated partial thromboplastin time (APTT) test was used to check the blood coagulation rate at different treatment times. Our findings showed that the amount of FVIII:Ag in healthy samples was high to low in seconds 4 > 8 > 16 > 0. The APTT results showed that APPJ increases the blood clot formation, but in hemophilic plasma samples, APPJ reduces the clot formation speed and FVIII:Ag concentration. Accordingly, the APPJ discharge elicited a slight increase in FVIII:Ag concentration in short-term treatment, which could be involved in blood coagulation. Moreover, the effects of APPJ discharge on other coagulation factors need further studies.

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We appreciate all the colleagues in the hemophilia center at Bu Ali Sina Hospital (Sari, Iran), who collaborated with us in this study. We would like to thank all the healthy and patient individuals who donated to us the blood samples, generously. We are grateful to our colleagues for critical reviews of the manuscript. This study was supported by a grant from the University of Mazandaran, dedicated to the MSc thesis of Elham Hasheminasabgorji (Registered No: 1394.11.03).

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Correspondence to Abasalt Hosseinzadeh Colagar.

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The authors declare that they have no conflict of interest.

Human and Animal Rights

The study protocol was approved by the ethics committee of the University of Mazandaran (#UMZ.REC.396006) and conducted in accordance with the Iran National Committee for Ethics in Biomedical Researches.

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Informed consent was obtained from all subjects.

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Hosseinzadeh Colagar, A., Hasheminasabgorji, E., Mohadjerani, M. et al. APPJ Discharge Effects on Human Factor VIII:Ag Level in Healthy and Hemophilia A Patient. Iran J Sci Technol Trans Sci 44, 13–19 (2020).

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  • Factor VIII
  • Coagulation
  • Hemophilia A
  • Fibrin clot formation
  • APPJ discharge