GC–MS Profiling and In Vitro Tyrosinase Inhibitory Activities of Bauhinia racemosa Lamk. and Bauhinia tomentosa Linn.

  • Pritipadma Panda
  • Tripti Sharma
  • Abhisek Pal
  • Goutam GhoshEmail author
Research paper


Bauhinia racemosa Lamk. and Bauhinia tomentosa linn. (Ceasalpiniaceae) leaves are used as holistic medicines mainly for inflammations and various skin disorders by the tribal sovereignty in Odisha ecoregion. This research work presents a study on chemical composition and tyrosinase inhibitory property of the methanol extract of leaves of Bauhinia racemosa (MBR) and Bauhinia tomentosa (MBT) with computational approaches. GC–MS technique, in-silico approach and in vitro tyrosinase inhibitory assay models were used for analysis of skin-whitening efficacies of these plant extracts. The major chemotype of the MBR was found to be myo-Inositol 4-C-methyl- (14.50%), whereas 3-O-Methyl-d-glucose (91.89%) was present in MBT as major compound. Tyrosinase inhibition was investigated against monophenolase (l-tyrosine). IC50 values of MBR and MBT were found to be 280 ± 0.9 and 610 ± 1.7 μg/mL, respectively. Molecular docking analysis of the binding of myo-Inositol, 4-C-methyl- and 3-O-Methyl-d-glucose with protein (PDB ID: 1wx2) showed an extensive hydrogen bond network with amino acid residues like ASN 191, ALA 202 and SER 206, THR 203, ASN 191, respectively. Both these compounds showed good binding affinities with target receptors and can be used as lead molecules for inhibition of tyrosinase. MBR and MBT might be suggested as a better source of skin-whitening agents for cosmetic and medicinal applications.


Bauhinia racemosa Bauhinia tomentosa Skin whitening GC–MS Docking 



This study was supported by School of Pharmaceutical Sciences, S‘O’A (Deemed to be University), Bhubaneswar.

Compliance with Ethical Standards

Conflict of interest

There are no conflicts of interest associated with this publication, and we confirm that the manuscript has been read and approved by all named authors.


  1. Aderogba MA, Mc Graw LJ, Ogundaini AO, Eloff JN (2008) Cytotoxicity study of antioxidant flavonoids from Bauhinia tomentosa leaf extracts. Niger J Nat Prod Med. Google Scholar
  2. Anandan A, Eswaran R, Doss A, Sangeetha G, Anand SP (2011) Chemical compounds investigation of Cassia auriculata leaves—a potential folklore medicinal plant. Bull Environ Pharmacol Life Sci 1:20–23Google Scholar
  3. Biswas R, Mukherjee PK, Chaudhary SK (2016) Tyrosinase inhibition kinetic studies of standardized extract of Berberis aristata. Nat Prod Res 30:1451–1454CrossRefGoogle Scholar
  4. Board N (2002) Hand book on herbal products (medicines, cosmetics, toiletries, perfumes), vol 2. National Institute of Industrial Research, New Delhi, p 58Google Scholar
  5. Chang YT, Rojania GR, Chung SK (2001) Inositol phospholipid pathway inhibitor and regulator. Exp Opin Ther Pat 11:1–15CrossRefGoogle Scholar
  6. Devaki K, Beulah U, Akila G, Narmadha R, Gopalakrishnan VK (2011) Glucose lowering effect of aqueous extract of Bauhinia tomentosa L. on alloxan induced type 2 diabetes mellitus in wistar albino rats. J Basic Clin Pharm 2:167–174Google Scholar
  7. Gopalakrishnan S, Vadivel E (2011) DNA cleavage studies of the ethanolic extracts of the bark of Bauhinia tomentosa l. and the whole plant of Mussaenda frondosa l. Int Res J Pharm 2:153–154Google Scholar
  8. Grosdidier A, Zoete V, Michielin O (2011) SwissDock, a protein-small molecule docking web service based on EADock DSS. Nucl Acids Res 39:W270–W277CrossRefGoogle Scholar
  9. Gupta M, Mazumder UK, Kumar RS, Gomathi P, Rajeshwar Y, Kakoti BB, Selven VT (2005) Anti-inflammatory, analgesic and antipyretic effects of methanol extract from Bauhinia racemosa stem bark in animal models. J Ethnopharmacol 98:267–273CrossRefGoogle Scholar
  10. Hayouni EA, Abedrabba M, Bouix M, Hamdi M (2007) The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chem 105:1126–1134CrossRefGoogle Scholar
  11. Ingle SS, Khobragade CN (2014) In silico drug docking and screening for the drug discovery of new tyrosinase inhibitors. J Pharm Res 6:704–708Google Scholar
  12. Jain R, Yadav N, Bhagchandani T, Jain SC (2013) A new pentacyclic phenol and other constituents from the root bark of Bauhinia racemosa Lamk. Nat Prod Res 27:1870–1876CrossRefGoogle Scholar
  13. Jayaraman A, Muthukrishnan S (2014) Free radical scavenging activity of ethanolic extract of Bauhinia tomentosa leaves collected from kolli hills (Tamilnadu). WJPR 3:4188–4199Google Scholar
  14. Kandhro A, Sherazi STH, Mahesar SA, Bhanger MI, Younis Talpur M, Rauf A (2008) GC–MS quantification of fatty acid profile including trans FA in the locally manufactured margarines of Pakistan. Food Chem 109:207–211CrossRefGoogle Scholar
  15. Khan MA, Sundaram MK, Hamza A, Quraishi U, Gunasekara D, Ramesh L, Goala P, Alami UA, Ansari MZ, Rizvi TA, Sharma C, Hussain A (2015) Sulforaphane reverses the expression of various tumor suppressor genes by targeting DNMT3B and HDAC1 in human cervical cancer cells. Evid Based Complement Alternat Med. Google Scholar
  16. Kim YJ, Uyama H (2005) Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future. Cell Mol Life Sci 62:1707–1723CrossRefGoogle Scholar
  17. Kiran A, Jain SK, Gupta A, Gupta SK, Bansal M, Sharma PK (2011) Antidiabetic activity of Bauhinia tomentosa Linn. roots extract in alloxan induced diabetic rats. Pharm Lett 3:256–259Google Scholar
  18. Kumar RS, Sivakumar T, Sunderam RS, Gupta M, Mazumdar UK, Gomathi P, Rajeshwar Y, Saravanan S, Kumar MS, Murugeshand K, Kumar KA (2005) Antioxidant and antimicrobial activities of Bauhinia racemosa L. stem bark. Braz J Med Biol Res 38:1015–1024CrossRefGoogle Scholar
  19. Kumar V, Rathore K, Jain P, Ahmed Z (2017) Biological activity of Bauhinia racemosa against diabetes and interlinked disorders like obesity and hyperlipidemia. Clin Phytosci 3:1–9CrossRefGoogle Scholar
  20. Manjula RR, Uppuluri S, Anand TJ, Adilakshmi V, Gandrapu P, Munagala A (2017) Wound healing effect of methanolic flower extract of Bauhinia tomentosa Linn. with emu oil in rats. J Pharmacogn Phytochem 6:247–253Google Scholar
  21. Mannangatti V, Ayyasamy B, Rangasamy M, Emin B, Natesan SK (2010) Anti-hyperglycemic and anti-lipidemic activity of ethanolic extract of Bauhinia tomentosa L. flower in normal and streptozotocin induced diabetic rats. J Global Pharma Technol 2:71–76Google Scholar
  22. Manosroiab A, Jantrawuta P, Akazawac H, Akihisac T, Manosroiab J (2010) Biological activities of phenolic compounds isolated from galls of Terminalia chebula Retz. (Combretaceae). Nat Prod Res 24:1915–1926CrossRefGoogle Scholar
  23. Matoba Y, Bando N, Oda K, Noda M, Higashikawa F, Kumagai T, Sugiyama M (2011) A molecular mechanism for copper transportation to tyrosinase that is assisted by a metallochaperone, caddie protein. J Biol Chem 286:30219–30231CrossRefGoogle Scholar
  24. Muhammad A, Sirat HM (2013) Antimicrobial, antityrosinase and brine shrimp lethality test of Bauhinia rufescens Lam (Fabaceae). J Coast Life Med 1:135–140Google Scholar
  25. Mukundan D, Mohankumar R, Vasanthakumari R (2017) Comparative study of synthesized silver and gold nanoparticles using leaves extract of Bauhinia tomentosa Linn and their anticancer efficacy. Bull Mater Sci 40:335–344CrossRefGoogle Scholar
  26. Nirmal SA, Laware RB, Rathi RA, Dhasade VV, Kuchekar BS (2011) Antihistaminic effect of Bauhinia racemosa leaves. J Young Pharm 3:129–131CrossRefGoogle Scholar
  27. Online Archive of U.S. Department of Agriculture, Agricultural Research Service. 1992–2016. Dr. Duke’s Phytochemical and Ethnobotanical Databases, Minnesota.
  28. Parvez S, Kang M, Chung HS, Bae H (2007) Naturally occurring tyrosinase inhibitors: mechanism and applications in skin health, cosmetics and agriculture industries. Phytother Res 2:805–816CrossRefGoogle Scholar
  29. Patidar LN, Bhargava P, Bhadauria RS, Ravichandran N (2011) Anti ulcer activity of aqueous extract of Bauhinia tomentosa Linn. leaves. ARPB 1:51–55Google Scholar
  30. Pattewar A, Kawtikwar P, Dawalbaje A, Reddy GK, Pawar P, Honmane P, Mitkari U, Jamkhande P (2013) Comparative evaluation of In-Vitro Antihelminthic activity of Bauhinia tomentosa. Int J Drug Dev Res 5:109–114Google Scholar
  31. Pettersen EF, Goddard TD, Huang CC, Cough GS, Greenblatt DM, Meng EC, Ferrin TE (2004) UCSF Chimera—a visualization system for exploratory research and analysis. J Comput Chem 25:1605–1612CrossRefGoogle Scholar
  32. Radha R, Vasantha VS, Pitchuman K (2016) Chemical constituents from the flowering buds of Bauhinia tomentosa Linn (fbbt). Nat Prod Res 30:1670–1674CrossRefGoogle Scholar
  33. Rashed K, Butnariu M (2014) Antimicrobial and antioxidant activities of Bauhinia racemosa Lam. and chemical content. Iran J Pharm Res 13:1073–1080Google Scholar
  34. Rhama S, Madhavan S (2012) In vitro antibacterial activity of Bauhinia tomentosa Linn. Leaf extracts. J Drug Deliv Ther 2:83–85Google Scholar
  35. Sebastian D, Fleming AT (2017) Synthesis of silver nanoparticles from Bauhinia acuminata aqueous leaf extract and molecular docking analysis of various cancer receptors. Int J Sci Res 6:50–55Google Scholar
  36. Srinivinasan GV, Sharanappa P, Leela NK, Sadashiva CT, Vijayan KK (2009) Chemical composition and antimicrobial activity of the essential oil of Leea indica (Burm.f.) Merr. Flowers. Nat Prod Radiance 8:488–499Google Scholar
  37. Uchida R, Ishikawa S, Tomoda H (2014) Inhibition of tyrosinase activity and melanin pigmentation by 2-hydroxytyrosol. Acta Pharm Sin B 4:141–145CrossRefGoogle Scholar
  38. Vijayalakshmi K, Nadhiya K, Haripriya D, Ranjani R (2014) In silico docking analysis of secondary metabolites of Bauhinia variegata and Garcinia cambogia with 4 as target for retinol binding protein obesity. Int J Pharmacogn Phytochem Res 6:636–642Google Scholar
  39. Zhang Y, Chen J, Wang L, Cao J, Xu L (2017) Chemical composition and biological activities of the essential oil from Rubus pungens var. oldhamii. Nat Prod Res 31:1454–1458CrossRefGoogle Scholar

Copyright information

© Shiraz University 2018

Authors and Affiliations

  1. 1.Department of Pharmacognosy, School of Pharmaceutical SciencesSiksha ‘O’ Anusandhan (Deemed to be University)BhubaneswarIndia

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