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Application of Fruit Wastes as Cost-Effective Carbon Sources for Biological Sulphate Reduction

  • Ali HussainEmail author
  • Muhammad Anwar Iqbal
  • Arshad Javid
  • Abdul Razaq
  • Sumaira Aslam
  • Ali Hasan
  • Muhammad Akmal
  • Javed Iqbal Qazi
Research Paper

Abstract

We conducted a study dealing with the economical application of sulphate-reducing bacteria (SRB) for the treatment of sulphate-rich wastewater. In this study, four types of frequently available fruit wastes were employed as carbon sources for economical cultivation of the SRB and consequent reduction of sulphate from artificially prepared sulphate-rich wastewater. The bacterial species employed in this study were isolated from a leading wastewater channel in Pakistan and characterized phenotypically as well as genotypically. The bacterial identities were proved after BLAST analysis and construction of phylogenetic tree. Among all of the SRB species employed for sulphate reduction, Desulfovibrio fructosovorans-HAQ2 was found as the leading sulphate reducer and reduced sulphate maximally to 29% (0.51 ± 0.02 g L−1), 76% (1.51 ± 0.07 g L−1), 41% (0.83 ± 0.02 g L−1), and 63% (1.25 ± 0.02 g L−1) using apple peelings, apple pomace, mango peelings, and watermelon rind, respectively, in a 60-day trial of anaerobic incubation. In the present study, apple pomace appeared as the most suitable carbon source only for Desulfovibrio fructosovorans-HAQ2, while any of the other three bacterial species could not reduce sulphate more than 34% using this substrate. However, on average, watermelon rind was found as the most appropriate carbon source for the cultivation of SRB and all the SRB species reduced sulphate efficiently in the range of 40–63% using this carbon source. Our findings of the present study prove the productive utility of fruit wastes for developing cost-effective and environmental-friendly remedial strategies.

Keywords

Cost-effective carbon sources Economical bioremediation Fruit wastes Sulphate-reducing bacteria Wastewater treatment 

Notes

Acknowledgements

Financial support of Higher Education Commission, Pakistan for funding the first author under the “Indigenous Ph.D. 5000 Fellowship Program” is highly acknowledged.

Compliance with Ethical Standards

Conflict of interest

The authors report no conflicts of interest.

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Copyright information

© Shiraz University 2017

Authors and Affiliations

  • Ali Hussain
    • 1
    Email author
  • Muhammad Anwar Iqbal
    • 2
  • Arshad Javid
    • 1
  • Abdul Razaq
    • 3
  • Sumaira Aslam
    • 4
  • Ali Hasan
    • 1
  • Muhammad Akmal
    • 5
  • Javed Iqbal Qazi
    • 6
  1. 1.Department of Wildlife and EcologyUniversity of Veterinary and Animal SciencesLahorePakistan
  2. 2.Department of ZoologyWomen University of Azad Jammu and KashmirBaghPakistan
  3. 3.Discipline of BotanyUniversity of Veterinary and Animal SciencesLahorePakistan
  4. 4.Department of ZoologyGovernment College Women UniversityFaisalabadPakistan
  5. 5.Department of Fisheries and AquacultureUniversity of Veterinary and Animal SciencesLahorePakistan
  6. 6.Department of ZoologyUniversity of the PunjabLahorePakistan

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