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Screening of Medicinal Plants for Potential Source of l-Asparaginase and Optimization of Conditions for Maximum Extraction and Assay of l-Asparaginase from Asparagus racemosus

  • K. BeulahEmail author
  • K. P. J. Hemalatha
Research Paper
  • 27 Downloads

Abstract

Different medicinal plants were screened for l-asparaginase. Asparagus racemosus exhibited highest activity (10.11 ± 0.12 U/ml) and specific activity (22.78 ± 0.28 U/mg of protein). A. racemosus was collected from Padmapuram garden of Araku valley, Visakhapatnam district. l-asparaginase activity was detected from the extracts of leaves and rhizome. Enzyme activity was maximum in the extract of rhizome (9.58 ± 0.12 U/ml) with specific activity (21.73 ± 0.28 U/mg of protein) compared with leaves which have the enzyme activity (1.85 ± 0.06 U/ml) with specific activity (2.68 ± 0.14 U/mg of protein). Optimum conditions for the extraction and assay of l-asparaginase from plant rhizomes were studied. Results showed maximum activity of l-asparaginase when the sample to buffer ratio was 3:6, sample weight (g)/buffer volume (ml) using 0.02 M of potassium phosphate buffer solution pH 8.2 at 40 °C. The l-asparaginase activity was 16.01 ± 0.06 U/ml with specific activity (26.81 ± 0.42 U/mg of protein) under optimum conditions.

Keywords

l-asparaginase Asparagus racemosus (ornamental) Optimum conditions Medicinal plants 

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Copyright information

© Shiraz University 2017

Authors and Affiliations

  1. 1.Department of BiochemistryAndhra universityVisakhapatnamIndia

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