Study on enzyme inhibition in biodiesel synthesis: effect of stepwise addition of methanol and removal of glycerol

  • Fahad Al BasirEmail author
  • Priti Kumar Roy
Original Article


Enzymatic transesterification is known as the most favourable and suitable method for biodiesel synthesis from vegetable oil or animal fat. Deactivation of enzyme during biodiesel synthesis remains a large barrier. Stepwise addition of alcohol and removal of glycerol (continuous or stepwise) have been adopted for minimizing the inhibition. In this article, a mathematical model is derived and inhibition of enzyme has been studied in biodiesel synthesis from Jatropha curcas oil. Sensitivity analysis of the model parameters has also been carried out to determine the most sensitive parameters which have a major role in the system functioning. Simulation results provide the validity of the proposed model. It has been shown that the proposed method minimizes the deactivation and enables enzyme reuse.


Transesterification Enzyme inhibition Mathematical model Impulsive differential equation Sensitivity analysis 



Fahad Al Basir acknowledges University Grants Commission, Government of India, for Dr. D.S. Kothari Postdoctoral Fellowship, File No.: MA/2016-17/0029. Priti Kumar Roy acknowledges Indo-Russian Joint Research Project, F No.: INT/RUS/RFBR/306 organized by DST-RFBR 2018, Government of India and Government of Russian Federation.

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.


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Copyright information

© The Joint Center on Global Change and Earth System Science of the University of Maryland and Beijing Normal University 2019

Authors and Affiliations

  1. 1.Department of MathematicsJadavpur UniversityKolkataIndia
  2. 2.Department of ZoologyVisva-Bharati UniversityBolpurIndia

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